Biomedical subjects

B Gründig

Publications and source records attributed to B Gründig.

4 recordsLinked to original sources

PubMed1999

A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer.

A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K(+)- and Na(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.

Biosensing Techniques↗

PubMed1997

Highly sensitive determination of hydrogen peroxide and peroxidase with tetrathiafulvalene-based electrodes and the application in immunosensing.

Redox mediators enable an efficient electron transfer between redox enzymes and the electrochemical surface of amperometric sensors. A stable and highly sensitive signal was obtained using a tetrathiafulvalene (TTF)-modified graphite electrode. In the presence of horseradish peroxidase (HRP), hydrogen peroxide (H2O2) was monitored with a detection limit of 7 nM at a potential of +20 mV versus a saturated calomel electrode (SCE). With a constant concentration of H2O2, the detection limit for HRP was found to be 150 pM. The accuracy of consecutive measurement of HRP in flow-through systems was improved by short-time polarizing the TTF-modified graphite electrode at +100 mV versus Ag/AgCl/3 M KCl. Using the TTF-modified graphite electrode in an immuno-sandwich approach, rabbit-immunoglobulin G was monitored in the range 5-100 ng/ml.

Animals↗

PubMed1997

The lipoxygenase sensor, a new approach in essential fatty acid determination in foods.

Both an enzyme electrode and enzyme column with immobilized lipoxygenase, respectively, were used for the determination of essential fatty acids. The former was applied in a batch system, the latter was part of a fully automated flow injection analysis (FIA)-system. The oxygen consumption due to the lipoxygenase catalysed oxygenation of essential fatty acids was monitored amperometrically. Both systems were compared with regard to linear ranges of the calibration plots, sensitivities, detection limits, apparent Michaelis-Menten constants and lifetimes. The enzyme electrode showed different sensitivities for linoleic and alpha-linolenic acids, the most common essential fatty acids. The reason for this was not a second oxygenation step by lipoxygenase in case of alpha-linolenic acid, but a different dialytic behaviour of the two substrates. Hence, only the FIA-system was used for the determination of these fatty acids in real matrices such as vegetable oils and margarines. In the presence of detergent the triglycerides of the hydrophobic food samples were converted into water soluble glycerol and free fatty acids by a 15 min incubation with a ready to use lipase/esterase-mix, thus avoiding the use of organic solvents for analysis. Results obtained by the enzymatic FIA-system were in excellent agreement with those obtained by standard gas chromatography.

Biosensing Techniques↗

PubMed1993

Development of a process-FIA system using mediator-modified enzyme electrodes.

A computer-controlled process-FIA system for monitoring industrial bioprocesses was developed using mediator-modified enzyme electrodes. The single-line FIA system was modified by replacing the mixing coil with a flexible operating sample dilution unit. By this way, the analyzer offers automatic procedures for self-calibration 'real-time' dilution and recalibration based on the current analyte concentration. In regard to the dynamic range of the sensors, the FIA system is able to self-adapting to any analyte concentration of the bioprocess. The technique was tested for control of glucose during microbial fed-batch processes of gluconic acid production.

Biosensing Techniques↗