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Development of a process-FIA system using mediator-modified enzyme electrodes.

B Gründig·B Strehlitz·H Kotte·K Ethner
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Abstract

A computer-controlled process-FIA system for monitoring industrial bioprocesses was developed using mediator-modified enzyme electrodes. The single-line FIA system was modified by replacing the mixing coil with a flexible operating sample dilution unit. By this way, the analyzer offers automatic procedures for self-calibration 'real-time' dilution and recalibration based on the current analyte concentration. In regard to the dynamic range of the sensors, the FIA system is able to self-adapting to any analyte concentration of the bioprocess. The technique was tested for control of glucose during microbial fed-batch processes of gluconic acid production.

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Biosensing TechniquesElectrochemistryElectrodesEnzymes, ImmobilizedGluconatesGlucoseGlucose OxidaseGlycoside Hydrolasesbeta-FructofuranosidaseJournal Article

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B Gründig, B Strehlitz, H Kotte, K Ethner. 1993. Development of a process-FIA system using mediator-modified enzyme electrodes.. https://doi.org/10.1016/0168-1656(93)90074-w

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