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Biomedical subjects

B Köhler

Publications and source records attributed to B Köhler.

At least 73 records · Page 4Linked to original sources
PubMed1989

[Spinal changes in neurofibromatosis in children].

A systematic radiologic study of the skeletal system of 72 children with different forms of neurofibromatosis (NF) revealed a high proportion of spinal defects. Scoliosis was found in 46%, anomalies of the vertebrae in an even higher percentage, particularly cleft spine in 25%, wedge-shaped vertebrae in 22% and scalloping of vertebrae in 40%. Radiographic examination of children with even minimal signs of NF was helpful in confirming the diagnosis, staging of patients and obtaining additional hints in regard to the long term prognosis of children with neurofibromatosis.

Adolescent↗
PubMed1989

[The use of ionizing radiation for the decontamination of salmonella-containing slaughtered broiler chickens and powdered eggs].

The effect of cobalt-60 gamma irradiation was investigated on the concentration of Salmonella (S.) typhimurium in artificial contaminated chicken carcasses and on the number of S. tennessee and S. agona in artificial contaminated spray-dried whole egg powder and liquid whole egg. Irradiation of carcasses and of liquid whole egg was carried out at deep frozen conditions between -10 degrees C and -18 degrees C and whole egg powder at 15-20 degrees C. The irradiation doses used where between 0.05 and 8.0 KGy. The D10-value of S. typhimurium in broiler chicks ranged between 0.57 and 0.74 and of S. agona respectively S. tennessee amounted to 0.95 and 1.07 in spray dried whole egg powder and 0.47 respectively 0.53 in whole egg content. Irradiation causes drastic reduction of physiological microflora in chicken carcasses. By radiation treatment using a dose of 4.0 KGy, germ count reductions by 3-5 decimal powers were achieved. Resistance of microbes increases in following succession: Pseudomonas spp., Salmonella spp., other Enterobacteriaceae spp., Micrococcus spp., Flavobacterium spp., yeasts, Bacillus spp., Streptomyces spp., fungi. Results of microbiological and sensorial examinations allow following conclusions: 1. Irradiation is highly effective against Salmonella. 2. Irradiation dose of 4.0 KGy is able to kill about 1 Million Salmonella bacteria per chicken. This guarantees safe elimination of natural occurring Salmonella bacteria in broiler chicken carcasses. 3. Irradiation of chicken carcasses up to 4.0 KGy causes no injury of quality, however storage longer than 4 months deteriorates sensorial qualities more than of unirradiated carcasses.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

[Hallervorden-Spatz syndrome with acanthocytosis].

The case of a female patient with infantile onset of progressive dystonia, disturbance of gait and dysarthria is presented. At age 7, the diagnosis of Hallervorden-Spatz disease was established by clinical findings including retinal pigment degeneration, basal ganglia hyperdensity on CT, and the rare association of acanthocytosis. The clinical course was followed over 15 years until the patient's death.

Acanthocytes↗
PubMed1987

[Cutaneous diphtheria from Africa].

A 31-year-old woman returned to her native Germany, after a two-year stay in Tanzania, suffering from cutaneous diphtheria which had spread to nose and throat. One of her three children, a four-year-old boy, also had Corynebacterium diphtheria in his throat, without any symptoms. Both mother and child had been immunized against diphtheria.

Adult↗
PubMed1987

Infection with the bacterium Streptococcus mutans and salivary IgA antibodies in mothers and their children.

Salivary IgA-antibody titres against different streptococcal antigens were measured in 64 mothers and their first-born children. Their presence and the level of Streptococcus mutans had been monitored for several years. The children had carried this bacterium for various lengths of time and now had varying numbers of salivary Strep. mutans. The subjects had participated in a longitudinal study completed one and a half years before, concerning prevention of early colonization of the child's mouth by Strep. mutans through measures undertaken by the mother. Whole-saliva samples were collected from all subjects and analysed by ELISA techniques for total IgA, and for IgA antibodies to whole cells of Strep. mutans serotype c and d, whole cells of Strep. sanguis, purified polysaccharide and protein antigens of Strep. mutans serotype c and d; a pool of Escherichia coli antigens was used as a control. No significant differences were found in the level of any of the specific IgA antibodies in children with different levels of indigenous Strep. mutans. A tendency to higher IgA antibody activity was found in children who had levels of Strep. mutans first detectable after three years of age and who had carried the bacterium for six months or less. The IgA antibody activity to Strep. mutans specific serotype c antigen and to whole cells of Strep. mutans serotype c (JC 2) was significantly higher in children with no caries experience when compared to children with more than two df-surfaces. This study offers no clear evidence for a protective role of salivary IgA antibodies against Strep. mutans colonization.

Adult↗
PubMed1987

Mutans streptococci, lactobacilli and caries prevalence in 11- and 12-year-old Icelandic children.

Since the caries prevalence has not declined in Iceland as it has in other European countries, it was of interest to study the presence of cariogenic bacteria in Icelandic children. The prevalence of salivary mutans streptococci and lactobacilli was examined in 217 11- and 12-yr-old Icelandic children and was related to their caries prevalence. In 2% of the children mutans streptococci were not found whereas 31% and 35% of the 11-yr-olds and 12-yr-olds respectively carried more than 10(6) CFU per ml saliva. Lactobacilli were not detected in 8% of the children whereas 28% of the 11-yr-olds and 23% of the 12-yr-olds had more than 10(5) CFU per ml saliva. The mean caries prevalence (DFS), initial caries included, for 11-yr-olds was 21.6 and for 12-yr-olds 28.8. Both mutans streptococci and lactobacilli were significantly correlated to each other as well as to caries prevalence. An increased number of these microorganisms, especially the mutans streptococci, were associated with an increased DFS. Children with high salivary counts of mutans streptococci and lactobacilli showed four times higher DFS than children with low numbers. The magnitude of salivary cariogenic bacteria as well as caries prevalence was found to resemble the situation in Sweden 10 yr ago. S. mutans (serotype c/e/f) was carried by all mutans streptococci positive children. S. sobrinus (serotype d/g) was found in 60 children (30.2%). Children with both S. mutans and S. sobrinus had significantly higher salivary counts of mutans streptococci and lactobacilli as well as caries prevalence than children with only S. mutans.

Child↗
PubMed1987

Comparison between a micromethod and a conventional method for estimation of salivary Streptococcus mutans.

Two methods for estimating the number of S. mutans in 501 paraffin-wax stimulated whole saliva samples were compared. A highly significant correlation was found between the results of a micropipette method and conventional agar plating. The micropipette method is simple, reliable and economical and is a suitable alternative to conventional plating for determination of the S. mutans infection level.

Bacteriological Techniques↗
PubMed1986

Enzyme immunoassay and Scatchard plot estimation of estrogen receptor in gynecological tumors.

Tissue samples of three endometrial carcinomas, seven ovarian carcinomas, and 24 mammary carcinomas were analyzed for estrogen receptor (ER) by enzyme immunoassay (EIA) and a conventional dextran-coated charcoal (DCC) method. In addition, ER and progesterone receptor were assayed by DCC only in 68 ovarian carcinoma specimens. All three endometrial cancer specimens showed elevated ER values by both assays. As with mammary cancers the ER-EIA values tend to be higher than DCC values. It was intriguing to note that negative Scatchard plot data resulted in residual ER levels in the EIA system. Also four ovarian cancer specimens with negative ER values by the DCC assay had detectable levels by ER-EIA, and three of these four had ER-EIA values less than or equal to 10 fmol/mg of protein. Of the ten breast cancers with negative DCC values, seven were less than or equal to 10 fmol/mg of protein by the ER-EIA. Good correlation (r = 0.88) between EIA and Scatchard plot data was calculated from ER data of 24 mammary carcinoma tissue samples. Receptor assays in 68 ovarian cancer patients indicate that ER determinations should become a useful tool in the management of patients bearing this carcinoma. In addition, receptor determinations may improve the possibility of predicting which well differentiated Stage I ovarian carcinomas are likely to recur. Present data combine to suggest that ER-EIA may become a useful diagnostic laboratory tool.

Breast Neoplasms↗