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Biomedical subjects

B Knopf

Publications and source records attributed to B Knopf.

At least 55 records · Page 3Linked to original sources
PubMed1989

[The effect of endogenous and exogenous factors on psoriasis vulgaris. A clinical study].

Using computerized data collected from 390 patients we investigated the impact of "endogeneous" factors (infections, menarche, menopause, gravidity, hormonal contraception, stress, nutrition, alcohol consumption, drugs) and "exogenous" factors (seasons, climate, sun exposition, Koebner phenomenon) on the course of psoriasis. We recommend certain rules of behaviour based up on our data and the reviewed literature. It is of importance to inform patients about their skin disease and positive as well as negative modifications of its course by endo- und exogenous factors. This is the only way to enable patients for an active selfcare.

Adult↗
PubMed1988

Alterations of epidermal lectin binding sites in acute contact dermatitis.

We investigated alterations of epidermal lectin binding sites, as well as of pemphigus and bullous pemphigoid antigens, in 28 human patch test reactions, both allergic (nickel, formaldehyde, N,N'-1,3-dimethylbutyl-N'-phenylenediamine) and irritant (sodium lauryl sulfate). The epidermal reactivity to a panel of lectins and human antisera to pemphigus vulgaris and bullous pemphigoid antigens was compared with samples obtained from normal skin and from skin under tape occlusion. We observed selective perturbations of lectin and antibody binding in acute contact dermatitis, whether allergic or irritant. The main findings were a loss of terminal sialic acids and longer bi- and triantennary mannosyl residues as well as a loss of pemphigus vulgaris antigen. The only difference between allergic and irritant patch test reactions was in topography of loss of WGA binding sites: in the former, it was most pronounced in the lower and middle epidermis, whereas in the latter it was seen in the uppermost subcorneal layers. Our findings support a common pathway of cell membrane alterations of keratinocytes in acute contact dermatitis.

Adolescent↗
PubMed1987

[PUVA irradiation decreases the binding of membrane and basement membrane markers to human epidermis in vitro].

PUVA effects on frozen sections of human epidermis were investigated (20 micrograms 8-methoxypsoralen/ml; UVA wavelength 365 +/- 5 nm; radiation dose between 2 X 10(2) and 2 X 10(2) J/m2). The quality of the glycocalyx was characterized by lectins labeled with FITC (HPA, PHA, LCA, PNA, SBA, RCA, GCA I, UEA I). Serum antibodies of bullous pemphigoid (BP) served as membrane markers for the basement membrane. Fixed BP antibodies were detected by FITC-antihuman IgG. The fluorescence intensity was markedly decreased at a dose of 2 X 10(5) J/m2; 2 X 10(4) J/m2 were less effective. At 2 X 10(3) J/m2, there was nearly no alteration of the fluorescence intensity observed. Changes were seen in the case of BP antibodies and lectins, except UEA I. The relatively high intensity (radiation dose) and the effects immediately observable suggest direct membrane alterations by PUVA in vitro.

Antibodies↗
PubMed1987

[Effect of dithranol therapy on membrane, basement membrane and nuclear markers in psoriasis lesions].

We investigated the effects of anti-psoriatic therapy with dithranol (1/20-1%) in salicylic acid (0.5%) in white petrolatum on lesional skin. FITC-labeled lectins and pemphigus vulgaris antibodies (PV) served as analytical means to study the glycocalyx. Antibodies of bullous pemphigoid (BP) were used as basal membrane markers. Nuclear antigens were recorded according to the binding of speckled, anti-nuclear antibodies (ANA) as well as antibodies to dsDNA. With some lectins, dithranol therapy resulted in pronounced fluorescence of the lower parts of the basal cells. ConA was fixed by the basal cell layer. To a lesser degree, ANA were fixed by nuclei of keratinocytes. PV antibodies were not fixed at all.

Adult↗
PubMed1987

[Characterization of lectin binding by psoriatic epidermis--dependence on pH].

The binding of five different lectins by normal as well as psoriatic lesional epidermis was investigated with regard to its dependence on the pH value. As we studied the reactions within the pH range of 3.0 to 9.66, we obtained the following results: lectin binding almost independent from pH (FITC-Con A, -PHA, HPA), fluorescence intensity dependent on pH (TRITC-Con A, FITC-LCA, -GCA), binding pattern dependent on pH (FITC-LCA, GCA). In contrast to normal epidermis, there was no pH dependence of the FITC-GCA binding pattern in psoriatic lesional epidermis.

Humans↗