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Biomedical subjects

B Knopf

Publications and source records attributed to B Knopf.

At least 73 records · Page 4Linked to original sources
PubMed1986

[Effect of neutral solvents on lectin binding of healthy and psoriatic epidermis].

We studied the influence of neutral solvents on epidermal lectin binding in normal and lesional psoriatic skin. 1% solution of Tween 80 or Triton X-100 had no effect on the binding of the following lectins: PHA, ConA, and LCA. The HPA staining of the str. spinosum in normal skin, however, was completely removed by neutral solvents. In psoriatic skin, str. spinosum staining with HPA was resistant. Our results are discussed in the light of an altered keratinocyte maturation in psoriasis.

Humans↗
PubMed1986

[Lectin binding of psoriatic skin].

The aim of our study was the characterization of epidermal lectin binding pattern in psoriatic vs. non-psoriatic skin in order to reveal possible alterations of the glycocalyx composition of psoriatic keratinocytes. We used fluoroisothiocyanate-labeled lectins of Canavalia ensiformis (ConA), Phaseolus vulgaris (PHA), Lens culinaris (LCA), and Helix pomatia (HPA). The binding pattern of psoriatic (involved and non-lesional) skin did not differ from the control samples in ConA, PHA, and LCA. In psoriasis, there was a prominent HPA binding to the dermo-epidermal junction and to a lesser degree, intercellular epidermal near the uppermost cell layers. In seborrheic keratosis, in contrast, there was no fluorescence of the dermo-epidermal junction or the first layers of the epidermis. The most pronounced binding was observed perinuclear in the upper epidermis. The results are discussed in the light of an altered keratinocyte maturation in psoriasis.

Humans↗
PubMed1986

[Characterization of subepidermal immune deposits in clinically unchanged skin in patients with lupus erythematosus (SLE)].

Subepidermal immune deposits containing IgG and C3 by 9 patients suffering from SLE were exposed to extreme excess of antigens in order to solubilize tissue-bound immune complexes. Sections of 4 micron were incubated at room temperature either with calf thymus DNA (0.3-30 mg/ml) or with human gamma-globulin (HGG) (1.6-16 mg/ml). The sections thus pretreated were compared with "native" samples by means of direct immunofluorescence technique. Only HGG resulted in drastic removal of IgG deposits along the dermoepidermal junction. DNA proved to be ineffective; deposits of C3 remained uninfluenced. Our results are discussed in the light of the composition of the subepidermal immune complex in SLE.

Antigen-Antibody Complex↗
PubMed1986

Differential effects of treatment with UV-light (365 nm) and 8-methoxypsoralen on chromosomes of healthy persons and psoriatic patients.

The effect of 8-methoxypsoralen (8-MOP, 5 X 10(-5) M), near UV-light of 365 wavelength (UVA, 1.5 J/cm2) and the combination of both (PUVA treatment) were studied on lymphocytes in vitro taken from healthy persons and patients with psoriasis vulgaris and psoriasis arthritis (psoriasis arthropathica). Chromosomes isolated from cell nuclei were visualized by means of Giemsa staining technique and analyzed for induction of chromosomal defects, i.e. premature centromere division (PCD), major coiling (MC), and formation of gaps and fragile sites. Exposure of nonpsoriatic lymphocytes to 8-MOP, UVA or PUVA increased the rate of PCD or MC generation. In experiments with psoriatic lymphocytes a much weaker effect was found, with a moderate increase of PCD and MC after UVA or PUVA treatment in the case of psoriasis vulgaris, and of MC after UVA treatment of psoriasis arthritis. On the average the number of chromosomes per metaphase plate displaying PCD did not exceed 10. No indication was obtained for the preference of certain chromosome groups or the appearance of "fragile sites". Under all experimental conditions the number of chromosome gaps ranged in the order of their spontaneous induction. Our findings suggest PCD and MC investigations as possible sensitive tools for diagnosing latent psoriasis and for refined analysis of psoriatic cells or chromosomes. However, more experiments along this line are needed.

Chromosomes, Human↗
PubMed1985

[Reactivation of the alpha 1-fetoprotein synthesis in systemic lupus erythematosus].

Serum concentrations of AFP have been determined in patients suffering from systemic lupus erythematosus (SLE) or other "collagenoses" using fluorescence ELISA adapted to the chamber analytical technique. 20% of the SLE patients showed elevated AFP levels (range 30-233 ng/ml), some of them repeatedly. Only 2 of the 14 patients without SLE had AFP levels above 30 ng/ml (37 resp. 40 ng/ml). In SLE sera there was a significant correlation of raised AFP to higher anti-dsDNA antibody concentrations (Mann-Whitney U-test: p = 0.042). The mean dosage of azathioprin and prednisone in patients with SLE did not significantly differ from the non-SLE group. We discuss the possible reactivation of AFP synthesis on account of SLE itself.

Alanine Transaminase↗
PubMed1985

[Alpha 1-fetoprotein--a marker of potential PUVA side-effects in psoriasis?].

Serum concentrations of alpha 1-fetoprotein (AFP) during PUVA treatment have been analyzed in 12 patients suffering from severe psoriasis or psoriatic arthritis. The results have been compared with AFP levels in psoriatics without PUVA treatment (group II), patients with psoriatic arthritis treated with methotrexate (group III), as well as dermatological patients without psoriasis (group IV). All groups revealed AFP levels below 25 ng/ml. Although a slight raise of AFP could be observed during PUVA therapy (p less than 0.005), we assume that AFP detection cannot be regarded as a marker of potential PUVA side-effects in psoriasis.

Adolescent↗