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Biomedical subjects

B Netzel

Publications and source records attributed to B Netzel.

34 records · Page 2Linked to original sources
PubMed1977

Transplantation of syngeneic bone marrow incubated with leucocyte antibodies. I. Suppression of lymphatic leukemia of syngeneic donor mice.

"Antileukemic autotransplantation" an approach to eradicate by antibodies residual leukemia in the bone marrow taken during remission and regrafted in relapse is proposed and is the basis of investigations on the elimination of leukemic cells transferred to syngeneic mice. Rabbit antiserum against mouse T cells killed over 99% of cells when incubated with 10-5 lymphocytes of a T cell AKR/J leukemia transferred subsequently to syngeneic hosts. Seventy-five per cent of the recipients of antibody-coated and complement-treated leukemic cells survived the observation period of 80 days. Recipients conditioned with 800 R and given syngeneic bone marrow mixed with 10-5 leukemic cells died within 11 days, while 40% survived the observation period of 100 days without leukemia if the leukemic bone marrow had been preincubated with anti-T cell globulin (ATCG) and complement. A detrimental effect of ATCG on normal T cells could only be demonstrated in thymectomized, irradiated recipients of syngeneic ATCG-treated marrow. These mice did not reject H-2-incompatible skin grafts in contrast to the controls without ATCG or without thymectomy. ATCG thus inhibited leukemic lymphocytes while sparing stem cells for hemopoietic reconstitution. At the same time it did not interfere with the recovery of cellular immunity after bone marrow transplantation.

Animals↗
PubMed1977

Population data on two new HLA-D determinants, EI and RE.

Homozygous typing cells (HTC) for two new HLA-D determinants, EI and RE, defined by family studies, are described. The HLA-D typing experiments among more than 300 unrelated individuals showed phenotype frequencies of 0.045 for EI and 0.098 for RE. Since the tested population was also typed for its HLA-A and -B alleles, linkage disequilibrium parameters could be calculated: HLA-D type EI was statistically significantly associated with HLA-B13 and Bw17, HLA-D type RE with HLA-Bw40. These data support the working hypothesis that both EI and RE are new alleles of the HLA-D series.

Alleles↗
PubMed1977

Specific absorbed antithymocyte globulin for incubation treatment in human marrow transplantation.

The experimental data show that absorption of ATG with liver-kidney homogenate and CLL and LCL cells stepwise removed the hemopoietic toxicity, whereas the specific activity against T lymphocytes remained. Although the mode of action of absorbed ATG could not be tested in the first clinical case, the successful experiments in rodents together with the fact that the incubation treatment was tolerated by the patient may provide a new way of preventing fatal GVH reactions in man.

Animals↗
PubMed1977

Effects of busulfan (BU) on hemopoiesis and immune reactivity in dogs.

Hematologic and immunosuppressive effects of BU were evaluated in 21 dogs. Twenty to 30 mg BU/kg produced severe but in most cases reversible myelotoxicity. All animals given 40 mg BU/kg died of marrow failure which was consistently reversed by the infusion of autologous marrow. Granulocytic progenitor cells (CFU-C) in the bone marrow of BU-treated dogs were severely depressed by day 7 after drug administration and in surviving animals showed only slow recovery to normal values. Cell-mediated immunity as determined by the in vitro reactivity of lymphocytes was normal after stimulation with T-cell mitogens (PHA, ConA), but impaired after stimulation with B-cell mitogens (PWM, SpA). Antibody formation against sheep red blood cells was not suppressed by BU-treatment.

Animals↗
PubMed1977

HLA-D typing with homozygous lymphoblastoid cell lines.

Lymphoblastoid cell lines (LCL) was established from peripheral blood lymphocytes (PBL) of 2 HLA-DW 3 homozygous siblings. HLA-D typing was performed with the homozygous LCL and PBL in a group of 51 unrelated individuals selected according to their HLA-A,B locus antigens. All the individuals who were typed positive for DW3 with PBL typing cells could also be typed positive with the LCL typing cells. Both homozygous LCL behave very similarly in the tested population. These experiments showed that the same HLA-D determinants were represented both on LCL and PBL cells and that LCL derived from MLC homozygous donors can be used for HLA-D typing.

Cell Line↗
PubMed1975

Linkage group HL-A-MLC-BF (properdin factor B). The site of the Bf locus at the immunogenetic linkage group on chromosome 6.

Genetic linkage between the HL-A and Bf loci could be confirmed in 43 families with 168 offspring. In 4 families, 5 recombinants out of 82 informative meiotic divisions were observed (r = 6.1%). The localisation of the Bf marker system was studied in 3 families with crossovers between HL-A and MLC. From these data the following map order of human chromosome 6 can be proposed: HL-A (1st locus) -- HL-A (2nd locus)--MLC-Bf---PGM(3). The fact that important components of the classical and alternate pathway of complement activation are governed by genes closely linked with HL-A and MLC loci leads to the proposition to include the Bf system into the Major Histocompatibility Complex in man.

Chromosome Mapping↗
PubMed1975

LD typing in man using cells frozen and stored in microtiter plates.

A method of freezing LD typing cells in microtiter plates was developed. Two times 10(5) lymphocytes in a small volume (15 mul) with a low DMSO concentration (5%) were directly frozen in a --70 degrees C freezer. These cells were used in the mixed lymphocyte culture after thawing by diluting step-by-step with serum and culture medium without washing. The kinetics obtained with cells frozen in microtiter plates were comparable to those with fresh cells. Typing for LD determinants was performed in a group of unrelated persons in a family study using the LD homozygous reference cell Pi frozen in microtiter plates.

Epitopes↗
PubMed1975

Effect of anti-Ia sera on mixed lymphocyte reaction in mice.

Anti-I-region associated (Ia) antigen sera specificially supress the stimulation reaction in unidirectional mouse MLR. No effect on the response was observed with these antisera. In addition, anti-H-2 sera did not affect stimulation, however, in some cases diminished the response. The data indicate that Ia antigens and LAD might be identical. Stimulation in mixed lymphocyte culture reaction (MLR) or graft vs host reaction has been shown to be governed by genes located in the I-region (immune response region) of the H-2 complex in mice (Bach et al. 1972 and Klein and Park 1973) and it appears that the H-2K and H-2D regions play only a minor role in that reaction. Recently, antigens have been described (Hauptfeld et. al. 1973, Götze et al. 1973, David et al. 1973) which are controlled by the I-region, so-called I-region associated equals Ia antigens. If these antigens are related to determinants important for the MLR, then one might predict inhibition of the MLR. Abbasi et al (1974) reported that poly-specific anti-H-2 alloantisera inhibited the H-2 complex controlled stimulation in MLR. Using antisera produced in I-region congenic strains, we have been able to show complete and specific inhibition of the stimulation in MLR, without affection of the response, thus indicating that Ia antigens and lymphocyte activating determinants might be identical.

Animals↗