Biomedical subjects
Publications and source records attributed to B Petermann.
If used effectively, performance appraisal systems keep employees focused on the vision and mission of the organization. Appraisal tools firmly grounded in desired behaviors can be effective in improving performance. The authors describe the steps and development of a Behavior Anchored Rating Scale (BARS) for registered nurses and give readers information for developing behaviorally based evaluation tools.
The combined addition of a heterologous anti-human C1q serum and an anti-IgG-L-chain (kappa) serum to only once washed human platelets potentiates their aggregation in vitro in comparison to the effect of the same antisera alone. The biological significance of this process is discussed.
Three methods for immune complex detection were used on 85 women who took oral contraceptives and on 21 who took none to test their sera for circulating immune complexes. The methods were ammonium sulphate precipitation as proposed by Beaumont et al., polyethylene glycol precipitation, and the 125J-Clq fixation test. - Significant rise in precipitable proteins, in excess to anything recordable from women with no oral contraceptives, was established from the oral-contraceptive group only by means of 25-per-cent ammonium sulphate precipitation. No significant difference between the two groups could be measured by the two other techniques. - Ammonium sulphate precipitation is not specific of immune complexes, and no difference between the two groups was recordable by means of the 125J-Clq fixation test, a technique more specific of immune complexes. The conclusion, therefore, was drawn that the proteins which had caused rise in ammonium sulphate precipitation had been proteins of unspecific precipitability rather than immune complexes. - Findings so far obtained are likely to suggest that administration of oral contraceptives will hardly induce formation of circulating immune complexes.
The immunochemical diagnostics of a lights chain myeloma is demonstrated by an example showing how the free light chains in the serum may be reliably identified by a specific antiserum, after sodium sulphate precipitation of immunoglobulins if a light chain proteinaemia is present.
Hemolyzates prepared from packed human red cells with 30 micrometer total calcium were employed as a means to examine the relationship between ATP consumption and lactate formation. Hemolyzates exposed to ultracentrifugation accumulate membrane fragments in the top layer yielding membrane-poor fractions in the buttom layers of the centrifuge tube. Lactate formation accompanied by ATP depletion amounts to 12 mumoles per ml and hour in complete hemolyzates fortified with NAD. Complexation of calcium results in about 50% inhibition of the lactate formation with a concomitant increase of ATP. Lactate formation is reduced in membrane-poor hemolyzates approximately concurrently to the extent of membrance removal which produces no discernible change in the glyceral-dehydephosphate dehydrogenase activity. 50--200 micrometer total calcium has no effect on the membrane-independent lactate formation which amounts to 1--2 mumoles per ml and hour. Triton X-100 seems to solubilise also the membrane components responsible for the high calcium-dependent ATP consumption which governs the lactate formation.
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