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Biomedical subjects

B Szynaka

Publications and source records attributed to B Szynaka.

36 records · Page 2Linked to original sources
PubMed1997

Morphometric and ultrastructural studies of the spinal sensory ganglia in the course of sciatic nerve regeneration in rats intoxicated with ethanol.

The aim of the study was to examine morphological changes in the spinal dorsal ganglia (from which sensory fibres of the sciatic nerve extend) in animals intoxicated with ethanol, in which the sciatic nerve was transected and then sutured. By means of morphometric methods using a computer system of picture analysis and in ultrastructural examinations it was found that ethanol administered to rats to drink for 3 months caused only slight morphological alterations in the sensory nerve cells of the spinal vertebral ganglia on the non-operated side. However, ethanol or its metabolites destroyed the regenerating sensory nerve cells of the spinal vertebral ganglia of the transected and then sutured sciatic nerve.

Alcoholic Intoxication↗
PubMed1997

Ultrastructural changes of Candida albicans under influence of cyclosporin A.

The aim of this study was the assessment of influence of cyclosporin A on ultrastructural changes of Candida albicans in vitro. Strains of Candida albicans isolated from woman with chronic vulvovaginitis were used. At the first part of experiment MIC (minimal inhibitory concentration) of cyclosporin on Sabouraud medium was assessed. Control plates were also prepared. From plates with Candida albicans there was observed its visual growth, the yeasts were prepared for electron transmission microscopic examines. It was found that ultrastructural changes of Candida albicans cells in the presence of Cyclosporin A. In particular, the cell wall was thicker and deteriorated. The organelles were altered in varying degrees and autolysis was observed.

Candida albicans↗
PubMed1997

Intermediate cells in the rat pancreas after chronic exposure to lead.

Ultrastructural analysis of the rat pancreas following the long-term exposure to lead revealed the presence of numerous intermediate cells in animals receiving lead acetate in doses of 500 ppm and 1000 ppm for 4 and 6 weeks. The cells included acinar-beta, beta-acinar and intermediate cells containing two kinds of endocrine granules- alpha and beta, and zymogen granules.

Animals↗
PubMed1996

Changes of myocardial capillary density in progression of experimental lung emphysema.

A morphometrical analysis of changes in the heart of rats with experimental lung emphysema due to single intratracheal administration of papain solution has been done. Special attention has been paid to quantitative changes of capillaries and to the cardiomyocyte diameter in respective regions of the myocardium after one, three and six months following the administration of papain. A histological and morphometrical analysis of changes in the lungs has also been carried out and partial pressures of oxygen and carbon dioxide in the arterial blood of the examined animals have been marked. It has been found that a single administration of papain caused emphysematous changes in the lungs, growing more intense until the third month of the experiment. The progression of these changes corresponded to a capillary density increase in the right ventricle, interventricular septum and in the subendocardial layer of the left ventricle. The increase in the number of capillaries was interpreted as the first adaptive stage of cor pulmonale development and explained by the occurrence of angiogenesis. The changes co-existed with a decrease in partial pressure of oxygen in the arterial blood. After six months of the experiment, a reduction in capillary density in the heart regions mentioned above and a simultaneous increase in the cardiomycyte transversal diameter were observed, being the exponents of evident myocardial hypertrophy. Development of ultrastructural changes in the hearts of rats has been analysed too. Simultaneous development of changes in the right ventricle muscle and in the subendocardial layer of the heart left ventricle myocardium was observed. The role of hypoxia as a one of essential factors responsible for the observed morphologic changes has been discussed.

Animals↗
PubMed1996

Chronic advanced pancreatitis--diagnosis, biochemical and ultrastructural changes and surgical procedures.

On the basis of 162 cases of chronic advanced pancreatitis seen at our Department during the last 15 years the diagnosis, indications and surgical procedures are described in this paper. Quantitative and qualitative changes of pancreatic collagen in chronic inflammation were also analysed. Results of these investigations were compared with histological and ultrastructural pictures of pancreatic tissue. Amongst 162 patients, 142 (88%) were operated upon. 71 underwent pancreatectomy or pancreatoduodenectomy, 14 anastomotic procedures and rest of them various operation of the bile ducts. Overall, 18 (13%) patients experienced significant complications after resectional procedures and 6 (4%) died as well after anastomotic procedures.

Basement Membrane↗
PubMed1996

The time course of ultrastructural changes in the secretory components of pancreatic acinar cells and trypsinogen activation in taurocholate pancreatitis in rats.

The purpose of this study was to compare a time course of ultrastructural changes of secretory compartment of acinar cells in the pancreas, and a pattern of trypsinogen activation during the course of taurocholate acute pancreatitis (AP) in rats. Acute pancreatitis was induced in 21 rats by injection 0.2 ml of 5% natrium taurocholate into the biliopancreatic duct. Control rats (n = 18) were sham operated (SO). The ultrastructural and biochemical (trypsinogen activation, free active [FAT] and total potential trypsin [TPT]) examinations were performed after 6, 12 and 18 h of AP or SO. Ultrastructural lesions of acinar cells comprised of disorganization of RER, enlargement of Golgi apparatus, changes in size, shape and number of zymogen granules. These alterations were most conspicuous after 6 h of AP and they were associated with maximal activation of trypsinogen. Biochemical changes gradually normalized at 12 to 18 h of AP, however the morphological lesions persisted at these intervals of time.

Acute Disease↗
PubMed1995

Megakaryocytes in the acute stage of experimental hemorrhagic shock. Part II. Megakaryocytic regulation of cell release from the bone marrow.

The contribution of MK to the release of other marrow cells to the blood was evaluated in rats subjected to experimental hemorrhagic shock. The analysis was based on the frequency of emperipolesis phenomenon in megakaryocytes, which was evaluated in a light microscope on marrow smears and in ultrastructural examinations. A considerable increase was found in the number of marrow cells in the MK cytoplasm in the animals examined, which indicates a significant part of MK in the release of these cells to the blood and in the marrow-blood barrier formation by MK.

Acute Disease↗
PubMed1995

Cor pulmonale in experimental lung emphysema. II. An ultrastructure of myocardium.

Development of ultrastructural changes in the hearts of rats with experimental lung emphysema, induced by proteolytic enzyme--papain, has been analysed. Simultaneous development of changes in the right ventricle muscle and in the subendocardial layer of the heart left ventricle myocardium was observed. After 1 month from papain administration in the muscle cells of the right ventricle a significant increase of mitochondria number and their polymorphism were observed. After 3 and 6 months similar changes were observed in both ventricles, however they were more pronounced in the cells of the right ventricle. Mitochondria showed significant damage in the form of clearning up of matrix and destruction of cristas and breaking of their membranes. An enlarged number of irregular intercalated discs was observed too. 6 months after papain administration in some of the right ventricle muscle cells a significant damage of myofibrils and increase in the number of sarcomeres as well as decrease of mitochondria number in these cells were noticed. Focally proliferation of connective tissue was observed too. The role of hypoxia as an essential factor, responsible for the observed morphologic changes has been discussed.

Animals↗
PubMed1994

Alveolar epithelial cells in experimental lung emphysema. Ultrastructural analysis of cells in situ in TEM.

A study was made of ultrastructural changes in alveolar epithelial cells after a single intratracheal papain injection. The experiment was carried out on male Wistar rats, of 180-220 g body weight. The animals were sacrificed after 1, 7, 14 and 28 days that followed proteolytic enzyme administration. The study revealed that the development of emphysematous changes in the rat lungs was accompanied by alterations in the epithelium of alveoli. In early stages of emphysema (up to 1 week), destructing changes dominated within the epithelial cells. Similar changes were observed in the endothelium of adjacent blood vessels. In later periods an increased number of type II pneumocytes were seen and focal accumulation of elastic and collagenic fibres was noticed in alveolar septa. Correlation was found between changes in the number of type II cells and their ultrastructure as well as between alveolar epithelium damage and alveolar septal interstitium alterations.

Animals↗
PubMed1994

Ultrastructure of the hypothalamo-neurohypophysial system in rats exposed to lead. I. Magnocellular nuclei.

The effect of lead on ultrastructure of supraoptic and paraventricular neurons was studied in rats allowed to drink lead acetate solution instead of water during 6 weeks. Generally, the neurosecretory neurons appeared to be sensitive to lead dependently on its concentrations, but the ultrastructural patterns of the individual neurons varied from cell to cell and suggested the co-existence of the neurons in different functional states such as the increased secretory activity, adaptive responses or cellular degeneration.

Animals↗
PubMed1994

Ultrastructure of the hypothalamo-neurohypophysial system in rats exposed to lead. II. Neurohypophysis.

Ultrastructure of neurohypophysis was examined in rats allowed to drink for 6 weeks only solution of lead acetate. It was found the increased number of neurosecretory granules in axonal terminals, the signs of granulolysis in Herring bodies and the presence of axonal terminals with atypical, heterogeneous contents. The possible mechanisms of alterations observed in the whole hypothalamoneurohypophysial system affected by lead were discussed.

Animals↗
PubMed1994

Ultrastructural evaluation of the rat parotid gland in the course of intoxication with lead acetate.

The purpose of this study was to evaluate the ultrastructural changes in the parotid gland of rats exposed to lead. The animals were given lead acetate in drinking water, at a concentration of 50 mg/dm3 Pb for periods of 2, 4 and 6 weeks. The ultrastructural examination showed that lead especially impaired mitochondria, caused accumulation of lipid droplets in the cytoplasm of acinar cells and inhibited the process of exocytosis of secretory granules. In the most extreme cases some acinar cells were destroyed.

Animals↗
PubMed1993

[Pneumonia in children and adolescents up to 18 years of age--a comparative analysis of the years 1976-1985 and 1986-1990].

An analysis was done of 1084 autopsies of children and adolescents up to 18 years of age carried out in the years 1986-1990. In the analysed period 631 cases were found of pneumonia (P) (58.2% autopsies) which was an increase of about 14% in relation to the years 1976-1985. Pneumonia was most frequently observed in the age range 1-12 months. It was the direct cause of death (primary pneumonia--PP) in 130 cases (11.9% autopsies) while in 501 cases (46.2%) it accompanied other primary diseases (secondary pneumonia--SP). In relation to the years 1976-1985 a decrease was observed of the per cent of autopsies with the diagnosis of PP (from 19.5% to 11.9%), as well as a significant increase of the per cent of SP) from 25.1% to 46.2%).

Adolescent↗
PubMedDate not supplied

Morphological changes in mitochondria and lysosomes of hepatocytes in acute intoxication with 2,4-dichlorophenoxyacetic acid (2,4-D).

The hepatotoxic effect of 2,4-dichlorophenoxyacetic acid (2,4-D) was investigated. Ultrastructural changes were evaluated under a transmission electron microscope. Certain histoenzymatic reactions were examined (to acid phosphatase according to Gomori (AP) and to succinic dehydrogenase (SD) according to Nachlas) in parenchymal cells of the rat liver in acute intoxication induced by this herbicide. The experiment used 60 male Wistar rats divided into two groups: I-control--18 animals and II--42 animals which received chemically pure 2,4-D acid by gastric gavage in a dose of 200 mg/kg b.w. The animals were sacrificed after 12, 24, 48 hours and 4, 10 and 30 days of the experiment. The results indicate that the administration of 2,4-D acid to rats in a dose inducing acute intoxication leads to histoenzymatic and ultrastructural changes in the liver, which suggest nonspecific reversible adaptive-type damage to parenchymal cells. The changes observed indicate disorders in energetic processes in hepatocytes and are morphological exponents of intense detoxicative processes.

2,4-Dichlorophenoxyacetic Acid↗
PubMedDate not supplied

Ultrastructural evaluation of the rat parotid gland after six-week-intoxication with lead acetate.

Clinical and experimental data point to the unfavorable effect of lead compounds on the oral cavity condition. The aim of this study was to analyse ultrastructural changes in the parotid gland of rats subjected to intoxication with lead acetate. The experiment was carried out on 24 Wistar rats divided into 4 groups, 6 animals in each. Group I animals were given aqueous solution of lead acetate of lead concentration 50 mg/dm3(50ppm), group II-500 mg/dm3(500ppm), group III-1,000 mg/dm3(1,000ppm). Control animals drank tap water. All the rats were sacrificed after six weeks of the experiment and material was collected for ultrastructural studies and evaluation of lead level in the tissue dry mass. A three fold increase in lead levels in the parotid gland was observed in group I and a tenfold increase in groups II and III. In all experimental ultrastructural examinations revealed groups in which lead impaired secretion of alveolar cells, damaged mitochondria and disturbed the lipid balance. The greatest destructive changes were observed in group III.

Animals↗
PubMedDate not supplied

Does lead acetate intoxication damage acinar cell nuclei in the rat pancreas? ultrastructural observations.

The aim of the paper is to make an ultrastructural analysis of the nuclei of the pancreatic acinar cells in the rat in the course of lead acetate intoxication. The experiment used 84 Wistar rats, divided into 3 experimental groups, and a control group. The experimental rats were given aqueous solution of lead acetate in a dose of 50 mg/dm3 in group I, 500 mg/dm3 in group II and 1000 mg/dm3 in group III. The control rats were given tap water. The animals were killed 2,4 and 6 weeks after the experiment. The pancreatic tissue was collected for ultrastructural analysis and lead level was determined in the blood and in dry pancreatic mass in an atomic absorption spectrometer. After 6 weeks of lead acetate administration the levels increased 5 to 10-fold compared with the control group. It was ultrastructurally demonstrated that a low dose of lead caused chromatin margination, extension of perinuclear space and folding of acinar cell nucleus contours. Chronic administration of high lead doses resulted in the formation of pseudoinclusions and intranuclear real inclusions of the lead-protein complex. The alterations provided evidence of the toxic effect of lead upon the acinar cells nuclei in the pancreas.

Animals↗