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Biomedical subjects

B V Pinegin

Publications and source records attributed to B V Pinegin.

At least 55 records · Page 3Linked to original sources
PubMed1991

[Disorders of the immune status in patients with acute diffuse peritonitis].

Analysis of various parameters of the immune system and non-specific resistance in patients with acute generalized peritonitis (AGP) has indicated that in AGP there is immunodeficiency involving all the links of immune defense and non-specific resistance. The T-cell immunity, primarily T helpers, is afflicted in AGP to the greatest extent. A magnitude of decreases in T helper counts may be used as a prognostic indicator in AGP. The severity of immune defense abnormalities correlates with the duration and intensity of intoxication, there is a "paralysis" of this defense in the terminal stage of AGP.

Acute Disease↗
PubMed1989

[Characteristics of cellular immunity in multiple sclerosis].

In 30 patients with definite multiple sclerosis a combined clinicoimmunological investigation was performed. The results of estimation of T-lymphocyte subpopulations by means of flow cytometry and monoclonal antibodies; of proliferative responses to PHA, PWM and Con A, to PHA with autoserum; of synthesis and reception of IL-2 containing material is discussed. A hypothesis of a cyclic course of the immunopathologic process is suggested (autoimmune and immunodeficiency stages).

Adult↗
PubMed1989

[A study of the ability of Bordetella pertussis toxin to induce the formation of B-suppressors].

Mouse spleen cells not adhering to the plastic surface and B-cells isolated from them were treated with B. pertussis toxin in vitro, washed and injected into recipients (allogeneic, syngeneic, intact or lethally irradiated) whose immune response to sheep red blood cells was then evaluated by Jerne's method. Treatment with B. pertussis toxin was shown to induce the development of immunosuppressive activity in intact spleen cells and in B-cells, to abolish the activity of memory B-cells and to enhance the suppressor activity of autoimmune mice. Supernatants obtained after autoimmune mice. Supernatants obtained after the 18- to 24-hour cultivation of spleen cells, previously treated with B. pertussis toxin for 60 minutes, suppressed the reaction of blast transformation of spleen cells to Con A and lipopolysaccharide and induced the appearance of immunosuppressive activity in intact spleen cells. The suppressing effect of the cells studied in this investigation may be linked with the ability of B. pertussis cells to stimulate the synthesis of cAMP, prostaglandins E and/or suppressor factors.

Animals↗
PubMed1988

[Influence of the oral administration of indigenous microorganisms on the resistance of mice to Salmonella infection].

The influence of the oral administration of killed bifidobacteria, lactobacteria, bacteroids and fusobacteria on the anti- Salmonella resistance of mice, infected orally with S. dublin, was studied. Bifidobacteria and lactobacteria were shown to produce a dose-dependent immunostimulating effect. The oral administration of killed bifidobacteria and lactobacteria led to the enhanced resistance of mice to Salmonella infection. The oral administration of killed bifidobacteria was conductive to the normalization of the intestinal microflora in dysbacteriosis developing in cases of Salmonella infection. Bacteroids and fusobacteria were found to possess no such effect.

Administration, Oral↗
PubMed1987

Normalizing effect of immunoglobulins in the treatment of endogenous infection and intestinal dysbacteriosis in irradiated mice.

It has been demonstrated in experiments on 2160 CBA mice and mice of mixed breed, irradiated by LD90/30, that therapeutical administration (subcutaneous or intraperitoneal) of immunoglobulins - homologous (polyglobulin, IgG, IgM) or heterologous - polyglobulin, IgG (from human, equine, canine blood) repeated three times, i.e., 2, 24 and 48 hours after irradiation, not only induces longer survival, but also shows a normalizing effect on the commonly developing dysbacteriosis and increased amount of intestinal microflora and, in addition, leads to suppression of postirradiation endogenous infection. Enterobacteria, enterococci, staphylococci, yeasts, disappear from the small intestine or their quantity decreases substantially in treated irradiated mice in contrast to untreated irradiated mice. In the large intestine, the amount of these organisms decreases considerably while the content of lactobacteria increases; no microbes can be found in the internal organs and in blood (or their content is small). Other conditions being equal, homologous immunoglobulins are more efficient in comparison with heterologous, this applying also to preparations containing normal antibodies to tissues.

Animals↗
PubMed1986

[Effect of antigen-induced B-suppressors on the development of B memory cells, carrier-specific T-helpers and antibody-producing cells].

(CBA X C57BL/6)F1 mice were immunized with 5 X 10(7) sheep red blood cells (SRBC). Seven days later plastic non-adherent B cells were prepared from spleens. They were designated as antigen-induced B-suppressors (AIBs). In double syngeneic transfer system AIBs stimulated the development of memory B cells (Bm) and suppressed the development of carrier-specific helper T cells (Th) and antibody-forming cells (AFC). AIBs possessing a surface Fc-receptor for human serum IgG (FcR+) inhibited the formation of all these cells. FcR- -AIBs failed to affect the development of Th and AFC, but stimulated the formation of Bm. In vitro preincubation of FcR+ -AIBs with mitomycin C or in vivo irradiation with 8 Gy abrogated the inhibitory effect on Bm and Th, but not AFC. It is suggested that FcR+ -AIBs suppress proliferation of Bm, Th and AFC precursors.

Animals↗
PubMed1986

[Effect of human immunoglobulins on microflora of the large intestine in nonspecific ulcerative colitis].

The use of commercial preparations of human immunoglobulin for the treatment of ulcerous colitis produces a positive effect on the microflora of the large intestine, contributing to the disappearance of Proteus, the lactose-negative forms of enterobacteria and the hemolytic variants of staphylococci, as well as to the increase of the amount of useful indigenous microflora (bifidobacteria and lactobacteria). The quantitative and qualitative improvement of the microflora leads, possibly, to the decrease of the intoxication of the body, improvement in the activity of the intestine and increased vitamin formation, thus giving a pronounced clinical effect and improvement in the endoscopic picture of the mucous membrane of the large intestine, peculiar for this disease.

Adult↗
PubMed1986

[Suppression of the immune response to ovalbumin in vivo using anti-idiotypic antibodies].

The effect of anti-idiotype antibodies (a-IdpI) on primary immune response to ovalbumin (OVA) was studied. A-IdpI against OVA antibodies were obtained with pI 6.2-6.7 from BALB/c mice. About 30-40% of IgG plaque cell formation (PCF) was inhibited if a-IdpI antiserum was added in situ, which served as a test for the evaluation of idiotype-positive (Id+) PCF. Up to 70% of common PCF and 70-80% of Id+ PCF were suppressed in mice that were injected with a-IdpI antibodies prior to immunization. This suppression was antigen- and allo-specific and depended upon the time of a-IdpI injection. If a-IdpI antibodies were disaggregated (DA) the Id+ suppression increased. A-IdpI antibodies also decreased IgE response to OVA, the suppression being most pronounced with the use of DA samples.

Animals↗
PubMed1986

[Use of immunoglobulin for treating patients with nonspecific ulcerative colitis].

The therapeutic effect of human immunoglobulins was assessed in 29 patients with nonspecific ulcerative colitis, as was their influence on intestinal flora. A 5 to 15 ml dose of immunoglobulin was administered intramuscularly every other day, 3 injections altogether. The clinical and endoscopic effect of treatment was positive in 21 patients (72.4%). Large-intestinal mucosal imprints were studied histologically in 17 patients. Inflammation subsided in 11 patients (64.7%), and glandular epithelial proliferation declined in 4 (23.5%). In the course of treatment, lactobacilli, and bifidobacteria were considerably increased in number, while opportunity organisms were decreased, a possible cause of the positive clinical effect.

Adult↗
PubMed1985

[Sensitivity of the process of the formation of antigen-induced B-suppressors to DNA synthesis inhibitors].

The injection of 5 X 10(7) sheep red blood cells (SRBC) to CBA and (CBA X C57BL/6)F1 mice was accompanied by the formation of antigen-induced B-suppressors (AIBs) in the spleen of the animals on day 7. When transferred to syngeneic intact recipients, AIBs inhibited their response to SRBC (control). Methotrexate in a dose of 2.5 mg/kg, cytozar in a dose of 1000 mg/kg and 5-fluorouracyl in a dose of 100 mg/kg, injected to donors simultaneously with SRBC (group A), had no influence on the number of IgM-forming cells and, when introduced on day 5 after the injection of SRBC, decreased the number of such cells in donors (group B) as determined by testing the donors' response on day 7. The suppressor activity of AIBs obtained from the donors of group A was equal to that of the control, while AIBs obtained from the donors of group B had lower suppressor activity in comparison with the control. These results suggest that the proliferation of the precursors of AIBs occurs between days 2 and 5 of immune response and the formation of AIBs themselves, between days 5 and 7.

Animals↗
PubMed1985

[Effect of normal and immune IgG, IgM and IgA on the intestinal microflora of mice with experimental dysbacteriosis].

Human IgG, IgM and IgA produce a pronounced protective effect, preventing enterobacteria from penetration into the mucous membrane of the proximal section of the small intestine of mice in antibiotic-induced dysbacteriosis. Normal mouse IgG and IgM, in contrast to IgA, are effective against mucosal enterobacteria of the small intestine. Immune mouse IgG, IgM and IgA show greater activity in protecting the mucous membrane than normal immunoglobulins of these classes.

Ampicillin↗
PubMed1985

[Effect of human immunoglobulins on mouse intestinal microflora in dysbacteriosis].

Human immunoglobulins, injected into mice subcutaneously after their irradiation or at the beginning of antibiotic therapy, protected the mucous membrane of the proximal section of the small intestine from the penetration of enterobacteria. The formation of the protective barrier was observed when immunoglobulin preparations with the titer of antibodies to Escherichia coli O14 between 1:16 and 1:256 was used. The preliminary exhaustion of immunoglobulin preparations with E. coli strain O14 led to the complete loss of their protective properties.

Ampicillin↗
PubMed1985

[Correction of intestinal microflora in chemotherapeutic dysbacteriosis using bifidobacterial and lactobacterial autologous strains].

The oral administration of kanamycin (40 mg/kg) or ampiox (500 mg/kg) to guinea pigs for 5 days led to disturbances in their normal intestinal microflora, manifested by a sharp decrease in the levels of lactobacteria and bifidobacteria, as well as by the appearance of large amounts of enterobacteria and enterococci, normally not detected in the proximal and distal sections of the intestinal tract. In adult volunteers receiving kanamycin orally in a dose of 40 mg/kg for 5 days disturbances in microbiocenosis also occurred: the amount of enterococci, staphylococci, lactobacteria and bifidobacteria considerably decreased, enterobacteria becoming the dominating microorganisms. Three oral administrations of bifidobacterial and lactobacterial autostrains immediately after the abolition of the antibiotic facilitated the rapid and effective restoration of the intestinal microflora.

Animals↗