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B V Pinegin

Publications and source records attributed to B V Pinegin.

At least 73 records · Page 4Linked to original sources
PubMed1984

[Effect of homologous serum globulin preparations on the intestinal automicroflora of mice after irradiation].

In experiments on 4000 noninbred and CBA mice the influence of the injections of alpha-, beta- and gamma-globusin, IgG and IgM, obtained from the sera of hemostimulated and intact mice, on the intestinal microflora after irradiation has been studied. The experiments have revealed that 3 subcutaneous or intraperitoneal injections of 1 mg per mouse, made 2, 24 and 48 hours after the irradiation of the animals with gamma-rays in a dose of 700 r, considerably reduce the intensity of the accumulation of opportunistic bacteria in the small and large intestines, commonly occurring in irradiated animals. A decrease in the number of lactobacteria is less pronounced. The preparations of globulin and IgG obtained from hemostimulated mice, i.e. enriched with normal tissue antibodies, have proved to be most effective.

Animals↗
PubMed1983

[Joint use of antibiotics and antibiotic-resistant bifidobacteria in treating acute intestinal infections].

Acute dysentery and other acute intestinal diseases with obscure etiology are accompanied by pronounced intestinal dysbacteriosis which is manifested, on one hand, by the increased levels of Escherichia, enterobacteria, staphylococci and by the appearance of Klebsiella and Proteus in large amounts and, on the other hand, by an essential decrease in the number of lactic acid streptococci, lactobacteria, bifidobacteria. The treatment of dysentery and other intestinal diseases with obscure etiology with antibiotics, while eliminating the infective agent, leads to the aggravation of intestinal dysbacteriosis. The combined use of antibiotics and antibiotic-resistant bifidobacteria in intestinal infections with obscure etiology contributes to the effective restoration of normal intestinal microflora already in the process of antibiotic therapy.

Actinomycetaceae↗
PubMed1983

[Adaptation of the antibiotic-resistant bifidobacteria in the intestines of normal and pathogen-free animals].

Antibiotic-resistant bifidobacteria have the ability to actively interact with mucosal tissue from the intestine of mice and rats in vitro and to adapt well in the intestinal tract of normal and germ-free rats, when introduced both with and without antibiotics; in some cases antibiotics enhance the ability of bifidobacteria to adapt in the intestine. The interaction between antibiotic-resistant bifidobacteria and mucosal tissue from the intestine remains unaffected by the prolonged action of 0.1M SDS and Tween-80 solutions, 0.1% trypsin and hyaluronidase. Antibiotic-resistant bifidobacteria introduced into the intestinal tract of rats in combination with gentamycin effectively inhibit the multiplication of exogenous enterobacteria infecting the digestive tract after the cessation of the antibiotic administration.

Animals↗
PubMed1983

[Use of antibiotic-resistant Bifidobacterium in treating ulcerative-necrotic enterocolitis in young children].

Ulceronecrotic colitis in premature children is accompanied by the development of pronounced intestinal dysbacteriosis characterized by a sharp increase in the number of Escherichia, enterococci, staphylococci and the appearance of opportunistic enterobacteria (Klebsiella, Serratia, Citrobacter, Proteus) in large amounts. Clinical convalescence was observed in 2 weeks in children receiving antibiotic-resistant bifidobacteria with antibiotics and in 3-4 weeks in children receiving commercial bifidobacteria with antibiotics, while children treated only with antibiotics showed no signs of clinical convalescence during the whole course of treatment. After the course of treatment the most effective recovery of the intestinal microflora was observed in the group of patients who had received antibiotics in combination with antibiotic-resistant bifidobacteria. It was manifested by a decrease in the number of Escherichia, Klebsiella, Proteus, Serratia, staphylococci and enterococci simultaneously with an increase in the number of endogenous lactobacilli and bifidobacteria. In those children who had received commercial bifidobacteria in combination with antibiotics or had been treated only with antibiotics the process of the recovery of the intestinal microflora was considerably less pronounced than in the patients of the first group; a decrease in the number of Escherichia, Klebsiella, Proteus and Serratia occurred to a lesser extent, and at the same time an essential increase in the number of enterococci and staphylococci was observed. The level of endogenous lactobacilli and bifidobacteria was considerably reduced. Antibiotic-resistant bifidobacteria actively took in the intestine of the patients. On day 5 after the course of treatment was over their level was (4.3 +/- 0.4) X 10(4) cells/g feces.

Actinomycetaceae↗
PubMed1982

[Effect of the combined use of antibiotic-resistant bifidobacteria and the corresponding antibiotics on the survivability of irradiated mice].

The combined administration of antibiotic-resistant bifidobacterial strains and the corresponding antibiotics (ampicillin, gentamycin or kanamycin) leads to a greater increase in the survival rate of irradiated mice than the administration of antibiotics only and is a promising method for the treatment of post-irradiation intestinal dysbacteriosis.

Actinomycetaceae↗
PubMed1982

[Characteristics of experimental antibiotic-induced dysbacteriosis].

Changes in the microflora of the large and small intestines in mice and guinea pigs after the oral administration of canamycin (a hardly absorbable antibiotic) and ampiox (an easily absorbable antibiotic) in different doses. The administration of these antibiotics in different doses (therapeutic, subtherapeutic and over therapeutic) led to an increase in the number of opportunistic microorganisms and the contamination of the small intestine by these organisms. These changes were also well pronounced in guinea pigs, normally having no enterobacteria. After the administration of the antibiotics was stopped, opportunistic microorganisms were gradually eliminated from the small intestine. The rate of decontamination depended on the administered dose of the antibiotic: the higher the dose was the longer the process of the decontamination of the small intestine lasted. An increase in the amount of opportunistic microbes in the large intestine and the decontamination of the small intestine occurred simultaneously with the decrease in the amount of lactobacilli and bifidobacteria in both the small and large intestines.

Ampicillin↗
PubMed1982

[Principles of decontaminating the small intestine with bacterial preparations in experimental antibiotic dysbacteriosis].

Introduction of antibiotic-resistant bifidobacterial and lactobacterial strains, separately or together, does not decrease the degree and duration of contamination of the small intestine by enterobacteria after oral administration of antibiotics in large doses. The use of antibiotic-resistant bifidobacterial and lactobacterial strains allows one to decrease contamination of the small intestine by enterobacteria, resulting from the administration of antibiotics in near-therapeutic doses. The therapeutic effectiveness of bacterial preparations with respect to antibiotic dysbacteriosis has been found to depend on the level of resistance to the administered antibiotic. Antibiotic-resistant strains of bifidobacteria decrease the number of opportunistic microorganisms in the intestine in cases of dysbacteriosis caused by irradiation and administration of antibiotics.

Actinomycetaceae↗
PubMed1982

[Interrelation between the presence in enterobacteria of distinct biological properties and their capacity to colonize the small intestine in the early stage of the development of postradiation dysbacteriosis].

An increase in the percentage of antibiotic resistant and hemolysin-producing enterobacteria occurring in the process of the development of postradiation intestinal dysbacteriosis was due to a sharp increase in the number of such bacteria among the representatives of the genera inhabiting the large intestine of mice (E. coli, E. aerogenes, E. cloacae, P. morganii) and to the appearance of antibiotic-resistant and hemolysin-producing strains in the intestinal tract; these strains belonged to the genera which had not been detected in the intestines of the animals before irradiation. In postirradiation dysbacteriosis a considerable decrease in the percentage of colicin-producing strains was observed. The dissemination of enterobacteria in the small intestine of irradiated mice occurred as the result of exogenous and endogenous infection.

Animals↗
PubMed1980

[Effect of antibiotics and bifiodobacterium preparations on the intestinal microflora of mice irradiated with gamma quanta].

The treatment of CBA mice, irradiated in a dose of 700 Gy with antibiotics (penicillin V, oxytetracycline, streptomycin) in combination with bacterial therapy (B. bifidum, strain 75-41) or with antibiotics alone led to the increased percentage of survivors among them in comparison with the control animals. At the same time the medical prognosis was better in cases of the combined treatment with antibiotics and bifidobacteria. This circumstance was due to the fact that the combined treatment with antibiotics and bifidobacteria, considerably facilitated the normalization of the microbial picture of the intestine; in this case the "eubiosis" of the intestinal tract was almost completely restored by the 22nd day after the irradiation, whereas in the treatment with antibiotics alone the inhibitory effect on the development of the opportunistic flora at a later period of the development of dysbacteriosis was less pronounced, while the number of lactic acid bacteria remained at the same low level as in the untreated mice.

Actinomycetaceae↗
PubMed1980

[Quantitative and qualitative characteristics of representatives of the enterobacteriaceae family in postradiation intestinal dysbacteriosis].

The irradiation of CBA mice with gamma quanta in a dose of 700 C/kg resulted in the development of postradiation intestinal dysbacteriosis in the animals. The dysbacteriosis was characterized by a considerable increase in the number of Escherichia and Proteus mirabilis in the large intestine and by the insemination of the small intestine with these microbial associations. Pr. vulgaris, Pr. morganii, Ent. aerogenes, Ent. cloacae, Citrobacter appeared in great numbers in the intestinal tract of the irradiated mice, while none of these organisms were found in the intact mice.

Animals↗
PubMed1980

[Microflora study of the intestinal cavity and mucosa normally and after gamma irradiation].

Lactobacilli and bifidobacteria are numerically prevalent in the microflora of the mucous membrane in mice and guinea-pigs. Some opportunistic microorganisms (Proteus, enterococci, aerobic bacilli, clostridia in mice; streptococci, yeast, enterococci in guinea-pigs) can mainly be found in the cavity of the large intestine, only small amounts of these microorganisms (10(2)), if any, are present on the mucous membrane. In the course of postradiation dysbacteriosis in mice and guinea pigs all opportunistic microorganisms which inhabit the cavity of the large intestine, proliferating actively, disseminate into the small intestine and, probably, invade the mucous membrane. Such microorganism as Escherichia, Proteus, Clostridium, normally absent in intact animals, appear in the intestine of guinea of guinea pigs subjected to irradiation. At the same time lactobacilli and bifidobacteria sharply decrease in number.

Animals↗
PubMed1979

[Activity of a number of enzymes in the splenic T- and B-lymphocytes of CBA strain mice].

The activity of some enzymes in T- and B-lymphocytes from the spleen of CBA mice was investigated. T-cell suspension was obtained by using a modified Kedar et al. method based on immunosorption. EAC-rosette forming cells were studied in the capacity of B-cells. The activity of all the enzymes examined proved to be higher in T-lymphocytes than in B lymphocytes; the greatest difference was noted in the activity of NADP.H2-diaphorase, and the least--in the activity of NAD.H2-diaphorase.

Animals↗
PubMed1979

[Detection of receptors for the IgG Fc fragment as well as for the C3 component of complement in the spleen T-lymphocytes of CBA mice].

The presence of receptors to the Fc fragment of IgG and to the C3 component in the complement of T lymphocytes in the spleen of CBA mice was studied. The study was made by the method based on Coons's indirect immunofluorescence test with the use of antithymocytic globulin, in combination with EA and EAC rossette-formation. A significant percentage of T lymphocytes was found to have the receptor to the Fc fragment of IgG, while only a small part of T lymphocytes was found to have the receptor to the C3 component of the complement.

Animals↗
PubMed1979

[Biological properties of antibiotic-resistant strains of lactic acid bacteria].

Lactic-acid bacteria (L. fermenti, L. acidophilus, L. delbruecki), when developing resistance to antibiotics, did not change their main biochemical, antagonistic properties and did not lose capacity for acid production. Only a decrease in their growth rate and a change in their sensitivity to the action of ultraviolet radiation were observed. Both initial and antibiotic-resistant strains were capable of taking on the mucous membrane of the large and small intestines in CBA mice.

Animals↗