Biomedical subjects

Beata Jankowska

Publications and source records attributed to Beata Jankowska.

8 recordsLinked to original sources

PubMed2003

[The influence of Aronia Melanocarpa Elliot and acetylcysteine on selected biochemical parameters of experimental animals with chronic lead acetate poisoning].

Lead mostly shows that it accumulates in bones in an insoluble phosphate form. Its toxicity grows with the deficiency of calcium, calciferol, irons and coppers in food. The examination was carried out on 40 female and male Wistar rats weighing 200-250 g. They lived in the animal quarters with a stable temperature and humidity. They were fed with standard fodder (Murigan) and water ad libitum. In the work carried out the effect of anthocyanins of Aronia Melanocarpa Elliot and acetylcysteine on the selected biochemical parameters of experimental animals with chronic lead acetate poisoning were examined. After administration anthocyanins statistically important substantially decreased the concentration of the products of the unsaturated fatty acids oxidation in urine and examined organs. Those anthocyanins also decreased excretion of delta-aminolevulinic acid and 8-hydroxy-2-deoxyguanosine in urine.

Animals↗

PubMed2002

[The effect of anthocyanins from Aronii melanocarpa and acetylcysteine on selected after-effects of lead acetate poisoning].

In recent years we have widely examined the results of protracted exposures on lead. Lead mostly shows that it accumulates in bones in an insoluble phosphate form. In much of the experimental research we observed that lead can cause reactive forms of oxygen and oxidative stress. The examination was carried out on 40 female and male Wistar rats weighing 200-250 g. They lived in the animal quarters with a stable temperature and humidity. They were fed with standard fodder (Murigan) and water ad libitum. In the work carried out the effect of anthocyanins of Aronia Melanocarpa Elliot and acetylcysteine on the selected parameters of oxidative stress of experimental animals with chronic lead acetate poisoning were examined. After administration anthocyanins substantially decreased the concentration of unsaturated fatty acid peroxidation and carbonyls in blood serum. At the same time we observed a significant decrease of 8-hydroxy-2'-deoxyguanosine in urine. Antocyanins from Aronia Melanocarpa appear to be a good substance for the diminishing of oxidative stress, these results caused with long-term administration of lead acetate.

8-Hydroxy-2'-Deoxyguanosine↗

PubMed2002

[Macrophages and the expression of endothelin in sulphur dioxide induced bronchitis in rats]].

The aim of the study was to evaluate the influence of SO2 on bronchial mucosa in rats. Our interest was focused on macrophages function and expression of endothelin. 20 animals were used in the study; 5 of them constituted the control group, 15 of them were submitted to long term SO2 irritation. In animals exposed to SO2 the BALF evaluation revealed the increase of inflammatory cells concentration: neutrophils, macrophages, lymphocytes. Electron microscope examination demonstrated that macrophages from study group were activated in comparison with healthy rats. Immunoreactive endothelin was found in bronchial specimens and mainly in alveolar macrophages (we used a computer-assisted system of image analysis). The expression of endothelin was significantly increased in study group in comparison with control group.

Air Pollutants↗

PubMedDate not supplied

[Sobriety testing in case of lack of cooperation of the examined person--the legal basis of the physician's activities].

The legal aspect of blood sample taking for alcohol concentration from suspicious, uncooperative persons is described in this paper. The Polish law allows for a possibility to take blood samples for alcohol concentration from suspects only by a physician or another skilled medical worker and only when consent is given to the medical procedure. The traffic act makes an exception for cases when the suspects are drivers and the suspicion concerns driving under the influence of alcohol. Taking blood samples is the medical worker' obligation in accordance with the law. The physician can refuse to do this only when the procedure threatens the life or health of the suspect.

Accidents, Traffic↗

PubMedDate not supplied

[Effect of ethylene glycol on antioxidative enzymes and lipid peroxidation activity in erythrocytes].

Investigations were aimed at demonstrating changes in the activity of enzymes forming an antioxidative barrier and oxidative stress parameters in erythrocytes of rats which were administrated ethylene glycol during 4 weeks. Superoxide dismutase activity (CuZn-SOD) was evaluated by the Misra and Fridovich method, catalase (EC1.11.1.6.) by the Beers and Sizer method and malonyl dialdehyde concentration (MDA) with Placer et all were assessed in the obtained material through the evaluation of TBARS compounds concentration. In the 4th week of the experiment a decrease in catalase (9.3 U/gHb to 5.7 U/gHb) and superoxide dismutase (2378 U/gHb to 1759 U/gHb) activity was observed. Malonyl dialdehyde concentration increased from the initial 0.14 mumol/gHb to 0.24 mumol/gHb. The investigations carried out have demonstrated that long-term intoxication with ethylene glycol leads to a constant generation of free radicals (increase of MDA concentration) and gradual exhaustion of the antioxidative system.

Animals↗

PubMedDate not supplied

[Selected alcohols on the pro- and anti-oxidative processes in rat erythrocytes].

In forensic medicine practice poisonings are rather frequent, and among them, those caused by fatal "substitution" of ethyl alcohol. One of the most frequently encountered "substitutes" for ethyl alcohol is methanol. The purpose of our research was to determine the concentration of malonic dialdehyde as the expression of lipid peroxidation and antioxidant enzyme activity after dosed chronic ethyl and methyl alcohol intoxication. The experiment was conducted on approx. 6 month-old male inbred Lewis rats each weighing approx. 250 g. Ethanol and methanol solution was given in the concentration 1.0 M. The control group of rats received water. Each experimental group numbered 30 rats, this number was divided into three sub-groups, which were put-down at 4, 8 and 12 weeks. The activity of superoxide dismutase (CuZu-SOD) was determined by the Misra-Fridovich method, catalase (CAT) by the Beers-Sizer method. The concentration of malonic dialdehyde (MDA) was determined using the method of Placer et al. by assessing the concentration of TBARS compounds. Results are expressed as a mean +/- SD. The paired Student's test for small groups were used. Superoxide dismutase SOD1 activity decreased compared with the control group throughout the duration of the experiment from 2212 U/gHb to 1676 U/gHb for ethanol and from 2212 U/gHb to 945 U/gHb for methanol. Catalase activity for methanol decreased from 9.1 U/gHb to 5.1 U/gHb, for ethanol to 7.4 U/gHb. In the 4th week of the experiment increase of malonyl dialdehyde concentration for methanol group was observed--from 0.14 umol/gHb to 0.34 umol/Hb; after 8th weeks it decreased to 0.2 umol/gHb and in the 12th week increased to 0.23 umol/gHb. For ethanol these changes was less visible and reached the level of 0.24 umol/l. The statistical processing of the results was performed on the basis of parametric tests (the t-Student test for small experiments) and computer software Statistica. The statistical significance was set for p<0.05.

Animals↗

PubMedDate not supplied

[Changes of the concentration of lipid peroxidation products and thiol groups in rat brains with long-term intoxication with methanol].

The aim of this paper was to define the influence of long-term methanol intoxication on the changes of end products of lipid peroxidation, reacting with thiobarbituric acid (TBA-reactive products) and free SH-group concentration in rat brains. The experiment was conducted on male Lewis rats. The experimental groups received a 1-molar methanol solution and the control group received tap water. The animals were killed after 4, 8 and 12 weeks of intoxication and their brains were collected for further examination, which encompassed measurement of the concentration of TBA-reactive products and free SH-groups. The revealed changes of TBA-reactive products and free SH-group concentration were not statistically significant.

Animals↗

PubMedDate not supplied

[Oxidative stress parameter analysis in rat brains during long-term ethanol intoxication].

The aim of this research was to define the influence of long-term ethanol intoxication on the changes in end products of lipid peroxidation, reacting with thiobarbituric acid (TBA-reactive products) and free SH-groups concentrations in rat brains. The experiment was conducted on male Lewis rats. The experimental groups received a 1-molar ethanol solution and the control group received tap water. The animals were killed after 4, 8 and 12 weeks of intoxication and their brains were collected for further examination, encompassing measurement of the concentration of TBA-reactive products and free SH-groups. A statistically significant increase in the concentration of TBA-reactive products in 4th week of intoxication and decrease in the concentration of free SH-groups in 8th and 12th week of intoxication--compared to the control group--was noticed.

Animals↗