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Biomedical subjects

Bin Zhou

Publications and source records attributed to Bin Zhou.

At least 91 records · Page 5Linked to original sources
PubMed2004

[Further study on heterogeneic basis of complement C8 beta deficiency].

OBJECTIVE: In Caucasian population, the most common molecular basis for C8 beta deficiency s a single C to T transition in exon 9 of C8 beta gene resulting in a stop codon. In previous family studies, two individuals were identified with C8 beta complete deficiency and were found to be only heterozygous for this mutation. This study was conducted by the present authors in search of other possible causes for these two C8 beta deficient individuals. METHODS: Using direct DNA sequence analysis of all exon-specific PCR products of the C8 beta gene from these two C8 beta deficient patients and their descendants. RESULTS: Two other C to T transitions at base 298 and 388 in exon 3 were detected, which could also create a termination codon. The descendants from one of the deficient patients were also analysed for the mutations, and it could be demonstrated that the two C to T mutations in exons 9 and 3 are segregating independently. CONCLUSION: These two mutations, which create a termination codon, are sufficient to explain the complete C8 beta deficiency in both patients.

Codon, Nonsense↗
PubMed2004

[Polymorphisms of five short tandem repeat systems in Chinese Zang population in Kangba area].

OBJECTIVE: To get preliminary genotype and allele frequency distributions of D1S549, D3S1754, D12S391, D12S375 and D18S865 loci in Chinese Zang (Tibetan) population in Kangba area and to validate more short tandam repeat (STR) systems for forensic application. METHODS: Fresh samples of venous blood from 100 unrelated individuals of Chinese Zang population in Kangba area were dropped on the filter paper. After they dried up, DNA were extracted by chelex-100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by PAG vertical electrophoresis and silver staining. RESULTS: Seven alleles were found at D1S549 locus, six alleles at D3S1754 locus, ten alleles at D12S391 locus, six alleles at D12S375 and six alleles at D18S865 locus. No deviations from Hardy-Weinberg equilibrium were observed. The heterozygosities observed were 0.86, 0.77, 0.81, 0.69, 0.80 for D1S549, D3S1754, D12S391, D12S375 and D18S865 respectively. The calculated values of the Power of Exclusion were 0.715, 0.545, 0.618, 0.413 and 0.599, and the values of the Power of Discrimination were 0.929, 0.838, 0.934, 0.886 and 0.890, respectively. CONCLUSION: All of the five loci in this study were useful for individual identification and paternity test, as well as for research purposes in genetics.

Alleles↗
PubMed2004

[Expression of vascular endothelial growth factor C in laryngeal squamous carcinoma and its role in lymphatic metastasis].

OBJECTIVE: To study the expression of vascular endothelial growth factor C (VEGF-C) in laryngeal squamous carcinoma (LSC) and its relationship to lymph node metastasis. METHODS: Paraffin-embedded specimens of primary laryngeal carcinomas from 60 patients subjected to operation were studied. VEGF-C expression was investigated using immunohistochemical staining. RESULTS: Positive immunohistochemical staining for VEGF-C protein was observed in some laryngeal squamous carcinoma cells (45/60), the VEGF-C antigen was identified in the cytoplasm of cancer cells. Furthermore, the expression of VEGF-C in laryngeal squamous carcinoma cells was significantly higher in lymph-node-positive groups than in node-negative groups, and the expression was significantly higher in poorly differentiated LSC tissues than in moderately and well differentiated LSC tissues. CONCLUSION: The above findings indicate a close correlation between VEGF-C expression and lymph node metastasis in laryngeal squamous carcinoma.

Adult↗
PubMed2004

[Study on the genetic polymorphisms of D8S1132 locus in Chinese Han, Mongolian and Thai populations].

OBJECTIVE: The aim of the present study was to obtain the population genetic data of short tandem repeat (STR) locus D8S1132 and study the difference of allelic frequency distributions among different populations. METHODS: A sample of 380 unrelated individuals from 3 populations (Han, Mongolian, Thai) were studied using single PCR followed by polyacrylamide gel electrophoresis and silver staining. RESULTS: Eleven allels and 42 genotypes were observed. The observed heterozygosity and the discrimination power were 85.0%-89.5% and 94.1%-95.5%, respectively. The genotype distributions of D8S1132 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious. CONCLUSION: The methods established are easy-to-perform and sensitive. The discrimination power and exclusion chances of this locus are desirable for forensic analysis.

Alleles↗
PubMed2004

[Sequence polymorphisms of the mitochondrial DNA control region in 100 Chengdu Hans in China].

OBJECTIVE: To study the genetic polymorphisms of the mitochondrial DNA (mtDNA) control region in Chengdu Han population. METHODS: Sequence polymorphisms of the mtDNA control region, hypervariable regions I and II from 100 unrelated Chinese Hans were determined by PCR and direct sequencing. RESULTS: Sequences of 404 nucleotides for hypervariable region I and 379 nucleotides for region II were obtained. Ninety-two and fifty variable sites were revealed in region I and region II respectively as compared to the reference sequence, and a total of 97 different genetic patterns from both the regions I and II were determined. The probability of identity was estimated at 1.84% for region I, 1.94% for region II, and 1.18% for both the regions. CONCLUSION: These results suggest that sequence polymorphism of mtDNA control region would be very useful in forensic practice as a marker for individual identification.

Base Sequence↗
PubMed2004

Inhibitors of 5-lipoxygenase inhibit expression of intercellular adhesion molecule-1 in human melanoma cells.

AIM: To study the effect of 5-lipoxygenase inhibitors on the expression of intercellular adhesion molecule-1 (ICAM-1) in melanoma cells. METHODS: ICAM-1 protein of human melanoma cell a375 was detected by enzyme-linked immunosorbent, flow cytometry and Western blot analysis. Level of ICAM-1 mRNA in a375 was evaluated by Northern blot analysis. Adhesion of a375 to endothelial cell EC304 was analyzed by isotopic tracing. RESULTS: 5-Lipoxygenase inhibitors nordihydroguaiaretic acid, AA861 and MK886, could suppress the expression of ICAM-1 protein as well as of its mRNA in a375 cells and reduce the adhesion of a375 to EC304. CONCLUSION: 5-Lipoxygenase inhibitors can inhibit the expression of ICAM-1 in human melanoma cells and may be valuable for treatment of melanoma metastasis.

Benzoquinones↗
PubMed2004

Effects of esculentoside A on production of interleukin-1, 2, and prostaglandin E2.

AIM: To investigate the influence of esculentoside A (EsA) on immunological function and its mechanism of anti-inflammation. METHODS: Interleukin-1 production was measured by thymocyte co-stimulating assay; the radioactivity of [(3)H]arachidonic acid (AA) was used to evaluate the release of AA; prostaglandin E2 production was measured with radioimmunoassay (RIA); IL-2 and IFN-gamma were detected by ELISA method. RESULTS: EsA (3-12 micromol/L)could potently inhibit the production of IL-1 and PGE(2) from both silent and LPS induced macrophages. EsA had no significant effect on the release of AA from murine macrophages. EsA could inhibit the production of IL-2 from murine lymphocytes induced by ConA, but not affect the production from silent lymphocytes. EsA showed no effect on the production of IFN-gamma from both silent and ConA induced lymphocytes. CONCLUSION: EsA could affect the immunological function through inhibiting the production of IL-2 from activated splenocytes and the inhibition of production of IL-1 and PGE(2) might be one of the anti-inflammation mechanisms of EsA.

Animals↗
PubMed2004

[A caregiver survey in Beijing, Xi'an, Shanghai and Chengdu: health services status for the elderly with dementia].

OBJECTIVE: In order to rationally allocate resources and to improve the quality of life of people with dementia, it is necessary to assess their current health services status. METHODS: A population-based door-to-door caregiver survey was conducted in Beijing, Xi'an, Shanghai and Chengdu in 1998. Totally 1 141 individuals with dementia were found among 34 807 residents aged 55 years and over on the basis of national prevalence study of dementia. Then 428 families with demented patients were randomly selected for questionare interview. Interviews were completed in 405 but refused by 23 families. Measurements included demographic characteristics of patients and their caregivers, perspective of the victim of the disease, current awareness of the disease and medical and welfare services provided. RESULTS: Among 405 cases of dementia (including 298 cases of AD and 81 cases of VaD), mean MMSE scores were 16.3 +/- 5.3 for 157 mild cases, 12.7 +/- 5.4 for 135 moderate cases, and 6.2 +/- 5.8 for 110 severe cases. Most patients (96%) were cared for at home by family members. Among caregivers, 189 (48.8%) considered the impairment of cognition, behavior and daily living activity in demented persons as a result of normal aging. Half of the caregivers spend over 8 hours each day looking after the patient. Whether the caregivers brought their relatives with dementia to a doctor was determined by disease severity, i.e. 8.3% for mild, 13.5% for moderate, and 19.4% for severe in 1996 to approximately 1997 and 14.4%, 25.6% and 33.6%, respectively in 1998-1999. Of those, only 26.9% reported receiving a dementia diagnosis, and only 21.3% received a recommedation to take medication. However, there was no established standard treatment and only 2% patient's used Ache-I. CONCLUSIONS: General education is needed to increase the public awareness of dementia. For patients with dementia, early diagnosis, early treatment and optimal care are important to improve their quality of life.

Activities of Daily Living↗
PubMed2004

[Association of APOA5 gene single nucleotide polymorphism with levels of lipids and coronary heart disease in Chinese].

OBJECTIVE: To investigate the single nucleotide polymorphism 4 (SNP4) of the apolipoprotein A5 (APOA5) gene possible association with coronary heart disease(CHD) and its distribution of in Chinese Han population. METHODS: APOA5 SNP4 genotyping was performed using polymerase chain reaction and Hae III restriction fragment length polymorphism analysis. RESULTS: APOA5 allelic frequencies of T, C were 0.435, 0.565 and 0.374, 0.626 in CHD group and control group, respectively. There is significant difference in allele and genotype frequencies between CHD group and control group (P<0.05). The levels of plasma high density lipoprotein in CHD patients with CC genotype were higher than those in CHD patients with other genotypes (P<0.01). The frequencies of T allele and C allele in Chinese was significantly different from those in Caucasians (0.374 vs 0.663, 0.626 vs 0.337, P<0.01). The C allele was much more common in Chinese population. CONCLUSION: The association is found between the Hae III polymorphism and CHD, There is a significant correlation between the CC genotype of the APOA5 and the levels of plasma high density lipoprotein-cholosteal in the CHD group.

Adult↗
PubMed2004

[Study on association between aldosterone synthase gene polymorphism and the left ventricular structure and function of patients with dilated cardiomyopathy in China].

OBJECTIVE: To investigate the association between a -344 C/T polymorphism of aldosterone synthase gene (CYP11B2) and the left ventricular structure and function of patients with dilated cardiomyopathy (DCM) in China. METHODS: Eighty-nine patients with DCM were genotyped by PCR-RFLP, and were divided into TT, TC, CC and TT+CC four groups by their genotypes, respectively. Left ventricular structure and function were assessed with echocardiography. RESULTS: There was no significant difference between four groups in left ventricular diameters, mass and ejection fraction in patients with DCM. CONCLUSION: The data were not in favor of a significant association between the aldosterone synthase gene CYP11B2 and the left ventricular structure and function in patients with DCM.

Adult↗
PubMed2004

[Association of 7alpha-hydroxylase gene polymorphism with levels of plasma lipids].

To study the distribution of Eco31I restriction polymorphism in nucleotide -204 of 7alpha-hydroxylase gene(CYP7A1)in Sichuan Han population of China and association of the polymorphism with coronary heart disease(CHD),CYP7A1 genotyping was performed by using PCR-RFLP approach in 183 CHD patients and 101 control subjects. 7alpha-hydroxylase gene allele frequencies of C,A were 0.840 and 0.160 in CHD group and 0.822 and 0.178 in control group,respectively. There was no significant difference in frequencies of allele and genotypes in A-204C polymorphism between CHD group and control group (P>0.05). However, in CHD patients there was significant difference in total cholesterol (TC) levels among CC,CA and AA genotypes (P<0.05) ,and the levels of high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in CHD patients with AA genotype were lower than those in CHD patients with CC and CA genotypes(P<0.05). In control group there was significant difference in TC levels between CC and CA genotypes (P<0.05) . The frequencies of C,A alleles at A-204C polymorphic site were significantly different from those reported in white people(P<0.05). The results indicating that no direct association was found between the A-204C polymorphism and CHD,but there was significant correlation between this polymorphism and the levels of TC ,and there was significant correlation in CHD patient group between this polymorphism and levels of HDL-C and LDL-C.

Adult↗
PubMed2004

[Expression of VEGF-C and VEGFR-3 in human laryngeal squamous cell carcinomas and cervical metastatic lymph node tissues].

OBJECTIVE: To study the gene expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in laryngeal squamous carcinoma and their relation with clinic pathologic features. METHOD: VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma (PLC) and cervical lymph node (CLN) were examined by reverse transcription polymerase chain reaction (RT-PCR). RESULT: The gene expression of VEGF-C and VEGFR-3 are different among normal laryngeal mucosa tissue, primary laryngeal carcinoma and cervical lymph node in the same patient. In primary laryngeal carcinoma, expression of VEGF-C, VEGFR-3 were significantly higher in lymph node positive group than in lymph node negative group and associated with histological grade of differentiation; The gene expression of VEGF-C, VEGFR-3 were not associated with age, sex, site, T stage. CONCLUSION: VEGF-C, VEGFR-3 have prognostic significance in laryngeal carcinoma.

Aged↗
PubMed2004

[Gene modification and high prokaryotic expression of porcine interferon alpha-1].

There are many E. coli rare codons in the gene of porcine interferon alpha-1. In order to obtain high expression of poIFN-alpha1 in E. coli, the cDNA encoded poIFN-alpha1 mature protein was synthesized using biased codons of E. coli without changing the original amino acid sequence and the terminator was changed as TAA. At the same time, Adenine and Thymine were used to the largest extent near the 5' terminus of poIFN-alpha1 mature protein gene. The synthesized gene was inserted into the Eco RI and Sal I site of the expression vector pRLC resulting pRLC-poIFN-alpha1. The poIFN-alpha1 is highly expressed in E. coli DH5alpha when the induction was carried out at 42 degrees C . The expressed poIFN-alpha1 account for 24.5% of the total cellular proteins and existed as inclusion body. The poIFN-alpha1 inclusion body was dissolved in 6mol/L guanidine chloride contained DTT and subsequently the denatured poIFN-alpha1 was re-natured by dilution in refolding buffer containing GSH and GSSH. In the present study it was found that the denatured poIFN-alpha1 was most efficiently re-natured in refolding buffer containing 1 mol/L guanidine chloride. In order to obtain pure protein, the concentrated re-natured poIFN-alpha1 was purified by Sephacryl S-200 chromatography. As a result, the purified poIFN-alpha1 is verified to be of high cytokine activity by inhibiting the cytopathic effect of vesicular stomatitis virus in MDBK cells, which is about 6.4 x 10(6) u/mg. This study paved the way for large-scale production of recombinant poIFN-alpha1 and its usage in virus disease control of pigs.

Animals↗
PubMed2003

Expression and characterization of a humanized cocaine-binding antibody.

The murine immunoglobulin G (IgG) cocaine-binding monoclonal antibody (mAb), GNC92H2, is notable for its exquisite specificity for cocaine, as opposed to chemically-related cocaine metabolites, and for its moderately high affinity (K(d) approximately 200 nM) for cocaine. Recently, we described the crystal structure of a mouse/human chimeric Fab construct at 2.3 A resolution. Herein, we report the successful framework humanization of a single-chain Fv (scFv) GNC92H2 construct without loss of affinity for cocaine. In brief, we compared the mAb GNC92H2 sequence to human antibody sequences, and used structure-based design to incorporate mutations (total = 49) that would humanize the framework region without affecting the overall shape of the binding pocket or the key cocaine-contact residues. The codons of the rationally designed sequence were optimized for E. coli expression, and the gene was synthesized by a de novo PCR reaction using 14 overlapping primers. Expression of the scFv construct was significantly improved in E. coli by fusion to thioredoxin. Intriguingly, this construct apparently refolds to form soluble active antibody in the reducing environment of the cytoplasm. Competitive ELISA and equilibrium dialysis demonstrated comparable binding activity between the humanized scFv and the whole IgG. The successful humanization of mAb GNC92H2 should enhance its potential therapeutic value by reducing its overall. immunogenicity.

Amino Acid Sequence↗