Bing Shi — Page 3 | PubMed Health

PubMed2002

[Effects of epidermal growth factors on the proliferation and metabolism of A/J mouse embryonic palatal cells].

OBJECTIVE: The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves. METHODS: The mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0.005, 0.010, 0.050, 0.100, 0.500, 1.000, 5.000, 10.000, 50.000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days and 5 days. RESULTS: EGFs stimulated DNA and PGE2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10.000 ng/ml. CONCLUSION: EGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.

Animals↗

PubMed2002

[A histological study on healing process of palatal wound with denuded bone restored with transplanted buccal or palatal mucosa].

OBJECTIVE: The purpose of this study was to observe the healing process of palate wound with denuded bone restored with transplanted buccal or palatal mucosa and to elucidate the mechanism of maxillary growth inhibition following palate repair. METHODS: 32 Japan white rabbits, 5 weeks old, were selected as the subjects for this study. They were divided into 4 groups at random. The rabbits in group I was the control without receiving any treatment. The rabbits in group II, III, IV was surgically denuded the bone of palate, and afterwards, the rabbits in group II were not received further restoration, but rabbits in group III and IV were restored with transplanted buccal and palatal mucosa respectively. From 2 to 14 weeks after surgery, at regular intervals, palatal wounds were observed by using a light microscope. Histological changes were also compared among different groups. RESULTS: It was found in group II that dense connective tissue was formed 2 weeks after the surgery, and Sharpey's fibers was formed between the scar and bone tissue 4 weeks after the surgery. However, no Sharpey's fiber was found in group III and group IV, and in the latter two groups, the histological character of tissue was similar to that of the control. CONCLUSION: Prevention of the attachment of Sharpey's fibers to the palatal bone could be effectively accomplished by covering the denuded palatal bone with the transplanted buccal or palatal mucosa.

Animals↗