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PubMed2003

[Treatment of posterior-communicating wide-necked aneurysms with endovascular stent and Guglielmi detachable coils].

OBJECTIVE: To summarize our clinical experience in treating posterior-communicating wide-necked aneurysms using endovascular stent and Guglielmi detachable coils (GDCs). METHODS: The coronary stents were implanted across the neck of 32 posterior-communication wide-necked aneurysms. Microcatheters were introduced into the aneurysm sac through stent mesh, GDCs were used to embolize aneurysms. RESULTS: All operations were successful. Total occlusion was achieved in 26 patients, over 90% in 4, 60 - 70% in 2. The patients were perfectly recovered with patency of the parent arteries. CONCLUSION: Endovascular therapy combined with stent implatation and GDC placement is a valid approach to the treatment of posterior-communication wide-necked aneurysms.

Adult↗

PubMed2002

Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells.

We report on the antileukemia effect of interleukin 2 (IL2) on different immune cells from 22 patients with chronic myeloid leukemia (CML). Bone marrow cells from these patients were first cultured in modified long-term bone marrow culture medium for several days, then separately cultured with lymphokine activated killer cells (LAK), cytokine-induced killer cells (CIK), and dendritic cell cocultured CIK (DC-CIK) for another 1-2 days. They were then detected for presence of the Philadelphia chromosome (Ph) by cytogenetic analysis and fluorescence in situ hybridization (FISH). The percentage of Ph-chromosome-positive cells in the bone marrow mononuclear cells after culturing with CIK and DC-CIK was significantly lower than that after culturing with IL2 or LAK. Our results demonstrate that cytogenetics and FISH are useful techniques for the evaluation of the anti-CML effect of immune cells and that CIK or DC-CIK can be appropriate candidates for adoptive immune cell therapy in vivo or for leukemia cell purging ex vivo.

Adult↗

PubMed1999

Rigid Molecular Rods with Cumulene-Bridged Polyphosphines: Synthesis, Electronic Communication, Molecular Photophysics, Mixed-Valence State, and X-ray Photoelectron Spectroscopic Study.

The synthesis, molecular photophysics, redox characteristics, and electronic interactions, as well as an X-ray photoelectron spectroscopic (XPS) study of a series of Ru(II) and Os(II) complexes with a polyphosphine/cumulene spacer, namely, 1,1',4,4'-tetrakis(diphenylphosphino)cumulene (C(4)P(4)), are studied and compared with the corresponding systems containing spacers with shorter sp carbon chain (C(n)()) lengths. Characterizations of all mono-, homo-, and heterobimetallic complexes with PF(6)(-) counteranions are accomplished using (1)H, (13)C, and (31)P{(1)H} NMR, fast atom bombardment (FAB/MS), and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF/MS) mass spectroscopy and elemental analysis. From the electrochemical study it is observed that the length of the C(n)() bridges has a profound influence on redox potentials and the electronic interaction between the two metal-based termini. XPS studies reveal that a simple change in carbon chain length affects the electron donation of the phosphine spacer to the metal-based termini. As a result, the redox potential of the Ru(II) or Os(II) center is shifted significantly. The comproportionation constant, K(c), is calculated as 1.3 x 10(7) (M = Ru(II)) or 4.5 x 10(10) (M = Os(II)) for homobimetallic [(bpy)(2)M(C(4)P(4))M(bpy)(2)](4+), suggesting a strong electronic communication across the C(4)P(4) spacer. However, the K(c) value is estimated to be ca. 4 for the corresponding complexes [(bpy)(2)M(C(3)P(4))M(bpy)(2)](4+) (M = Ru, Os; C(3)P(4) = 1,1',3,3'-tetrakis(diphenylphosphino)allene), indicative of a system with electronic isolation between the two termini. In heterobimetallic [(bpy)(2)Ru(C(n)()P(4))Os(bpy)(2)](4+) (n = 3, 4), the energy transfer from Ru(II) to Os(II) is found to be very efficient, with rate constants k(en) of ca. 3 x 10(9) s(-)(1) (n = 3) and 1 x 10(11) s(-)(1) (n = 4). The increased value of k(en) upon the change from C(3) to C(4) can be explained by the increase in the electronic communication across spacers. Detailed studies and calculations have revealed a Dexter-type of mechanism for the triplet energy transfer in the system.

Journal Article↗