Biomedical subjects

C C Lin

Publications and source records attributed to C C Lin.

At least 73 records · Page 4Linked to original sources

PubMed1994

Hepatoprotective effects of Taiwan folk medicine: Ixeris chinensis (Thunb.) Nak. on experimental liver injuries.

The hepatoprotective effects of Ixeris chinensis (Thunb.) Nak. were studied on acute hepatitis induced in mice by a single dose of carbon tetrachloride (31.25 microliters/kg, ip) or acetaminophen (600 mg/kg, ip), and in rats by a single dose of beta-D-galactosamine (188 mg/kg, ip). Hepatoprotective activity was monitored by estimating the serum transaminases (SGOT and SGPT) levels and histopathological changes in the livers of experimental animals. The Ixeris chinensis (Thunb.) Nak. extracts significantly inhibited the acute elevation of serum transaminases. Histopathologically, the crude I. chinensis extract significantly ameliorated hepatotoxin-induced histopathological changes in the livers of experimental animals. All pharmacological and histopathological effects of Ixeris chinensis (Thunb.) Nak. were compared with Bupleurum chinense DC., which has been previously reported as a treatment herb for hepatitis.

Alanine Transaminase↗

PubMed1994

Radionuclide venography by subcutaneous injection of Tc-99m pertechnetate at acupuncture point K-3: a case report.

Recently, we developed a new method of radionuclide venography of lower limbs, namely SC-RNV, by subcutaneous injection of Tc-99m pertechnetate at acupuncture points K-3. In this study, we applied this method to evaluate the venous drainage of lower limbs in a patient with severe varicose veins and edematous swelling of the left lower extremity. For comparison, an ascending radionuclide venography by intravenous injection of Tc-99m MAA (IV-RNV) was also done. The SC-RNV showed normal venous drainage of the right side but complete obstruction of the left popliteal vein with a prominent collateral flow, compatible to the findings of IV-RNV. The findings in this case again demonstrated that SC-RNV may be useful as an alternative method of venography as previously suggested.

Acupuncture Points↗

PubMed1994

Endolymphatic sac ballooning surgery for Menière's disease.

A newly developed technique (endolymphatic sac ballooning surgery [ESBS]), based on revisional sac surgery observations, was conducted on 135 patients with intractable Meniere's disease. The surgery involves the insertion and fan-folding of a thin Silastic sheet in the endolymphatic sac (ES) lumen to increase its volume. The results of this sac ballooning surgery proved more effective for treatment of incapacitating Meniere's disease symptom complex than did primary ES surgery (ESS) with either the nonballooning sac techniques we employed previously or ESBS revision operations in which primary surgery used nonexpansion techniques. It appears that ESBS, with its preservation of ES integrity and expansion of its lumen, may indeed prevent surgically induced damage and be more efficacious in the drainage of hydropic endolymph. The efficacy of ESS remains controversial because of the failure to understand its pathophysiologic basis. Nevertheless, we are confident that if the ES is definitively delineated, as evidenced by turbid or clear fluid or bubbles when entered, and enlargement of its lumen with maintenance of the sac's integrity is verified during surgery, ESS is undoubtedly efficacious treatment for intractable Meniere's disease. Furthermore, we maintain that equitable judgment may only be rendered when reports evaluating the efficacy of ESS include definitive identification, delineation, and drainage of the sac.

Adolescent↗

PubMed1994

Single-lumen endotracheal intubated anaesthesia for thoracoscopic sympathectomy--experience of 719 cases.

A total of 719 thoracoscopic sympathicotomies were performed at our hospital from October, 1989 to December, 1992. We have been practicing single-lumen endotracheal intubation for general anaesthesia in all of our cases. We will review our experience and discuss our anaesthetic technique and the intraoperative complications encountered as well as post-operative pain control. General anaesthesia with controlled manual ventilation assisted the surgeon well and created clear access for electro-cauterisation of the sympathetic chain. Thirty patients were randomly chosen for arterial blood gas analysis. There was no evidence of systemic hypoxaemia or clinically significant carbon dioxide retention throughout the surgery or afterwards in the recovery room. In our experience of 719 cases, single-lumen endotracheal intubated anaesthesia is safe and economic for thoracoscopic sympathicotomy.

Adolescent↗

PubMed1994

Intraoperative cardiac arrest: a rare complication of T2,3-sympathicotomy for treatment of hyperhidrosis palmaris. Two case reports.

Endoscopic surgery, including thoracoscopic sympathicotomy for treatment of hyperhidrosis, is thought to be safe and entail fewer complications as compared with open methods. A total of more than 719 patients with hyperhidrosis have undergone thoracoscopic T2,3-sympathicotomy for treatment of hyperhidrosis at Tainan Municipal Hospital since October 1, 1989. Most of the complications have been minor; however, two of the patients suffered from sudden cardiac arrest at the time when the left T2,3-sympathetic nerve trunk was transected by the thoracoscopic method. Vigorous cardiopulmonary resuscitation was performed and both patients recovered completely without any sequelae. The purpose of this paper was to discuss the possible mechanism of cardiac arrest in thoracoscopic sympathicotomy, and to emphasise this rare but potentially fatal complication in the treatment of hyperhidrosis palmaris.

Adolescent↗

PubMed1994

Bilateral thoracoscopic lower sympathetic-splanchnicectomy for upper abdominal cancer pain.

Upper abdominal pain secondary to cancer may cause great disability to patients. Surgical sympathetic-splanchnicectomy, or coeliac plexus block, has been used to relieve intractable upper abdominal cancer pain from stomach, liver, gallbladder or pancreas for a long time. There are, however, intrinsic disadvantages when performing these procedures. Due to our long experience of therapeutic thoracoscopy, we recently performed bilateral thoracoscopic sympathetic-splanchnicectomy on 14 patients (eight male and six female) for relief of pain from primary or metastatic upper abdominal cancers. Most of them were satisfied with the immediate pain relief after the operation. In this new era of therapeutic endoscopy, endoscopic surgery will gradually take the place of open methods in treatment of many diseases. It can be anticipated that thoracoscopic lower sympathetic-splanchnicectomy is undoubtedly a good alternative method for management of intractable upper abdominal pain.

Abdominal Pain↗

PubMed1994

[Anti-inflammatory effect of triptolide].

Triptolide (Tri) was isolated from Tripterygium wilfordii Hook f. Tri 0.1-0.2 mg.kg-1 sc or 0.15-0.3 mg.kg-1 ig inhibited markedly the increased vascular permeability induced by ip 0.7% HAc in mice. Tri 0.05-0.1 mg.kg-1 ip or 0.15-0.3 mg.kg-1 ig inhibited hind paws swelling induced by sc 0.15 ml carrageenan and also inhibited the same swelling induced by sc 2.5% formaldehyde 0.1 ml in rats. Tri 0.05-0.1 mg.kg-1 ip inhibited markedly proliferation of granuloma induced by sc implantation of cotton-pellets in rats, but 0.2 mg.kg-1 ip can not inhibit the same swelling induced by sc 0.15 ml carrageenan in adrenalectomized rats. Tri 0.2 mg.kg-1 ip decreased markedly weight of thymus. Tri 0.2 mg.kg-1 ip, but 0.1 mg.kg-1 ip did not reduced the content of ascorbic acid of adrenal gland in rats. Tri 0.2 mg.kg-1 ip did not decrease the pro-staglandin E content in inflammatory tissues. These results indicate that high dose of Tri can stimulate the pituitary-adrenal axis.

Adrenal Glands↗

PubMed1994

Treatment of bleeding peptic ulcer by bilateral truncal vagotomy via a transpleural thoracoscopic approach and laparoscopic pyloromyotomy.

Six cases of recurrent bleeding duodenal ulcer were treated by bilateral truncal vagotomy via a right transpleural thoracoscopic approach and laparoscopic pyloromyotomy at Tainan Municipal Hospital. The vagus nerves were completely transected, which was proved by pathology. Basal acid output and insulin-stimulated maximum acid output were reduced by 68%-77% and 87%-92%, respectively, after vagotomy. On the other hand, there was no delay in gastric emptying on the basis of either patients' complaints or an upper gastrointestinal series of x-rays. Transpleural thoracoscopic bilateral truncal vagotomy and laparoscopic pyloromyotomy are safe, effective and offer more complete transection of the vagus nerves in treatment of complicated peptic ulcer. In addition, this new technique provides shorter hospitalization and an earlier convalescence.

Adult↗

PubMed1994

Cardiovascular collapse during gynecologic laparoscopy complicated by pulmonary edema: report of a case.

Although gynecologic laparoscopic surgery has recently become a routine and widespread operative procedure in Taiwan, the potential risks and complications in the clinical practice of laparoscopy should not be overlooked. Whilst the incidence of complications are rare, they can sometimes be serious, even life-threatening. This case report presents a woman with ruptured endometrioma, who developed sudden-onset cardiovascular collapse during laparoscopic procedure, in which carbon dioxide was used for insufflation. After resuscitation including cardioversion, her vital functions were restored; pulmonary edema developed soon afterwards but was resolved with conservative treatment. We discuss the possible causes of cardiovascular collapse during laparoscopic procedure and the management of resulting complications.

Adult↗

PubMed1994

Effect of nasal CPAP on ventilatory drive in normocapnic and hypercapnic patients with obstructive sleep apnoea syndrome.

The purpose of this study was to evaluate the effect of nasal continuous positive airway pressure (CPAP) on the abnormal ventilatory drive in hypercapnic patients with the obstructive sleep apnoea syndrome (OSAS). Six patients with hypercapnic OSAS (Group I) and 24 patients with eucapnic OSAS (Group II) were studied. All patients had arterial blood gas analysis, overnight sleep studies, and an assessment of ventilatory drive (progressive hyperoxic hypercapnic response and progressive isocapnic hypoxic ventilatory response) prior to and during nasal CPAP therapy (at 2 weeks and 1 month of treatment). Nasal CPAP effectively improved the hypopnoea/apnoea index in both groups (Group I: 87 +/- 14 vs 8 +/- 4; Group II: 63 +/- 17 vs 6 +/- 3). Both hypercapnic and hypoxic ventilatory drive before treatment were significantly impaired in Group I as compared to Group II. Both the slope and baseline level of the ventilatory response and the mouth occlusion pressure (P0.1) improved significantly after 2 weeks of nasal CPAP therapy in Group I, with normalization of arterial carbon dioxide tension (PaCO2) (6.3 +/- 0.2 to 5.2 +/- 0.4 kPa). We conclude that it is possible to completely correct the abnormal ventilatory drive in hypercapnic OSAS patients within 14 days of initiating nasal CPAP therapy.

Female↗

PubMed1994

Sintered porous DP-bioactive glass and hydroxyapatite as bone substitute.

There is extensive experimental and surgical experience with the use of bone tissue to fill defects in the skeleton, to bridge non-union sites, and to pack defects in bone created from cyst curettage. DP-bioactive glass with a chemical composition of Na2O 8.4%, SiO2 39.6%, P2O5 12% and CaO 40% has been reported as an alternative bone substitute of high mechanical strength, good biocompatibility. and which has a tight bond with living tissue. The bonding layer between DP-bioactive glass and bone tissue was considered to be formed by dissolution of calcium and phosphate ions from the DP-bioactive glass into the surrounding body fluids. The biological hydroxyapatite was suspected to deposit directly onto the bonding layer. In order to confirm the interaction between the DP-bioactive glass and bone tissue, the developed bioactive glass was implanted into rabbit femur condyle for 2-32 weeks. The histological evaluation of DP-bioactive glass as a bone substitute was also investigated in the study. Porous hydroxyapatite bioceramic was used in the control group and the results were compared with those of DP-bioactive glass. The interface between the DP-bioactive glass and bone tissue examined with SEM-EPMA showed that the bioactive glass formed a reaction layer on the surface within 2 weeks after operation and formed a direct bond with natural bone. The elements contained in the bioactive glass apparently interdiffuse with the living bone and biological hydroxyapatite deposited onto the diffusion area, which was proved by EPMA and TEM. After implantation for over 8 weeks, the DP-bioactive glass was gradually biodegraded and absorbed by the living bone. Histological examination using the optical microscope showed that osteocytes grow into the inside of the DP-bioactive glass and the bioactive glass would be expected to be a part of bone.

Animals↗

PubMed1994

Congenital duodenal web in late infancy and childhood: a report of two cases.

Congenital duodenal webs are rare lesions, usually detected during early infancy because of signs of high intestinal obstruction. The occasional patient escapes both symptoms and detection until adolescence or adulthood. This report concerns two cases of congenital duodenal web at different ages and with different clinical manifestations. Case 1, a six-month-old male, was admitted because of abdominal distention and vomiting. Case 2, a 13-year-old boy, was referred here for further evaluation of recurring seizure attacks, elevated blood urea nitrogen and creatinine and hyponatremia. Duodenotomy and excision of the web performed for both patients. Complete amelioration of all symptoms was then observed at Outpatient Clinic follow-up for one year.

Adolescent↗

PubMed1994

Efficacy and safety of radiofrequency catheter ablation for paroxysmal supraventricular tachycardias.

From January 1992 to June 1993, 100 consecutive patients with clinically documented paroxysmal supraventricular tachycardias underwent radiofrequency catheter ablation. Group 1 consisted of 46 patients (male:female = 9:37, age: 46 +/- 13 years) with slow-fast atrioventricular nodal reentrant tachycardia. Radiofrequency current was aimed at the slow pathway area which was identified by both anatomical and electrophysiological methods. A mean application of 8 +/- 9 was delivered at a mean power of 22 +/- 4 watts with a mean duration of 21 +/- 3 seconds. Selective ablation of slow pathway conduction was achieved in 28 patients and modification of slow pathway conduction in 12 patients. Antegrade fast pathway conduction was ablated in 3 patients, and retrograde fast pathway conduction in 1. Mean peak CPK was 156 +/- 117 IU/L after ablation. Neither AV block nor clinical recurrence was found during 9.7 +/- 5.1 months follow up. Group 2 consisted of 54 patients with accessory pathway (AP) mediated atrioventricular reciprocating tachycardia. For 35 patients (M:F = 21:14, age: 40 +/- 12 years) who had left-sided accessory pathway, catheter ablation was approached by the retrograde trans-aortic technique in 33 patients and by the transseptal left atrial approach through patent foramen ovale in 2 patients. The mean energy delivered was 28 +/- 5 watts for a duration of 27 +/- 12 sec and 9 +/- 8 applications. The accessory pathway conduction was successfully ablated in 30 patients (86%). Mean peak CPK was 392 +/- 534 IU/L. Cardiac tamponade occurred in 1 patient and transient cerebral ischemia in another, but without mortality. No clinical recurrence was found during 9 +/- 4 months follow-up. Nineteen patients (M:F = 7:12, age: 36 +/- 11 years) had right-sided AP. The mean energy required for successful ablation was 30 +/- 4 watts for a duration of 35 +/- 15 sec and 12 +/- 9 applications. Mean peak CPK was 147 +/- 70 IU/L. Clinical recurrence occurred in 3 patients (15.8%), 1 of them had subsequent successive ablation. The overall immediate procedure success rate for right-sided AP was 65%. In conclusion, radiofrequency catheter ablation is a safe and effective treatment modality for patients with paroxysmal supraventricular tachycardias.

Adolescent↗

PubMed1994

The circadian variations of blood pressure and heart rate in patients with mitral valve prolapse.

It is well known that both normal subjects and hypertensives manifest a distinct nocturnal fall of arterial blood pressure and heart rate. But till now there has been no report about the diurnal change of blood pressure and heart rate in MVP patients. In this report we studied the circadian variations of blood pressure and heart rate in 18 symptomatic MVP patients (mean age 32 +/- 10 years) and compared them with those in 18 age- and sex-matched normal control subjects (mean age 35 +/- 9 years). The MVP group presented a statistically significant blunting of the nocturnal fall in systolic blood pressure (4.2 +/- 3.6% vs 9.7 +/- 3.7%, p = 0.0002). No obvious difference in the nocturnal fall in heart rate was noted between both groups (20.2 +/- 6.0% vs 18.8 +/- 5.6%, p = 0.4290). In conclusion, the circadian variation of blood pressure was blunted in patients with mitral valve prolapse. The mechanism is uncertain and further studies are necessary to clarify it.

Adult↗

PubMed1993

Expression and nature of the alkaline phosphatase gene in cultured osteosarcoma cells.

The molecular mechanism for the differences in specific activity of alkaline phosphatase in six human osteosarcoma cell lines was investigated. Five of the lines expressed only the tissue-non-specific or liver/bone/kidney isoenzyme of alkaline phosphatase. The sixth line had the lowest levels of alkaline phosphatase and this was determined to be a mixture of liver/bone/kidney isoenzyme and at least one other form. The mRNA of liver/bone/kidney alkaline phosphatase was identified by Northern analysis in the three cell lines that expressed the largest amount of alkaline phosphatase catalytic activity. This mRNA was indistinguishable in size from that seen in control mRNA from normal kidney (2.5 kb). Southern analysis demonstrated that EcoRI or HindIII restriction fragment patterns and the intensity of the bands, of the liver/bone/kidney alkaline phosphatase gene in the osteosarcoma cell lines were identical to that of the control DNA from normal peripheral blood leukocytes. Thus, the gene coding for liver/bone/kidney alkaline phosphatase appears to be intact in all of these osteosarcoma cells and it is unlikely that rearrangement, deletion or amplification of the gene is responsible for its activation or inactivation. Slot blot analysis revealed varying amounts of the transcripts of the liver/bone/kidney isoenzyme in each of the cell lines. The best fit line of a plot of the log of the level of mRNA of alkaline phosphatase vs. the log of the specific activity of liver/bone/kidney alkaline phosphatase was constructed. This gave a Pearson correlation coefficient of 0.92 (P < 0.008), demonstrating a significant relationship between the two variables. It is likely that the regulation of alkaline phosphatase activity is at the transcriptional process rather than the translational or post-translational processes and that the specific activity of the enzyme may be controlled by the amount of steady-state mRNA of the liver/bone/kidney isoenzyme.

Alkaline Phosphatase↗

PubMed1993

Binding of suprofen to human serum albumin. Role of the suprofen carboxyl group.

The binding of suprofen (SP), a non-steroidal anti-inflammatory drug of the arylpropionic acid class, and its methyl ester derivative (SPM) to human serum albumin (HSA) was studied by dialysis and spectroscopic techniques. In spite of the remarkable differences in the physicochemical properties of SP and SPM, the binding of each molecule to HSA was quantitatively very similar. Thermodynamic analysis suggests that the interaction of SP with HSA may be caused by electrostatic as well as hydrophobic forces, whereas the interactions with SPM may be explained by hydrophobic and van der Waals forces. Similarities in the difference UV absorption spectra between ligand-detergent micelle and -HSA systems indicate that the SP and SPM molecules are inserted into a hydrophobic crevice on HSA. The same studies suggest that the carboxyl group of SP interacts with a cationic sub-site which is closely associated with the SP binding site. Proton relaxation rate measurements indicate that the thiophen ring and propanoate portion of the SP molecule is the major binding site for HSA. The locations of SP and SPM binding sites were identified by using fluorescence probes which bind to a known site on HSA. The displacement data implied that SP primarily binds to Site II, while the high affinity site of SPM as well as low affinity site of SP are at the warfarin binding site in the Site I area. From binding data with chemically modified HSA derivatives, it is likely that highly reactive tyrosine (Tyr) and lysine (Lys) residues, which may be Tyr-411 and Lys-195, are specifically involved in SP binding. In contrast, these two residues are clearly separated from the SPM binding site. The binding of SP and SPM is independent of conformational changes on HSA that accompany N-B transition. There is evidence that the carboxyl group may play a crucial role in the high affinity binding processes of SP to HSA.

2-Hydroxy-5-nitrobenzyl Bromide↗

PubMed1993

Determination of ceftibuten in sputum by column-switching high-performance liquid chromatography on-line with thermospray mass spectrometry.

A column-switching high-performance liquid chromatographic assay on-line with thermospray mass spectrometric detection is described for the determination of ceftibuten, an oral cephalosporin antibiotic, in human sputum. The method does not require sample pretreatment and provides increased selectivity not available from the previously reported method with UV detection. The thermospray mass spectrometric detection conditions were optimized for ceftibuten. The technique has a detection limit of 0.50 micrograms/mL and allows precise, simple, and accurate determination of ceftibuten in sputum over the range 0.50-10.00 micrograms/mL.

Ceftibuten↗

PubMed1993

Isolation and identification of a novel tandemly repeated DNA sequence in the centromeric region of human chromosome 8.

EcoRI subclones, designated as 50E1 and 50E4, were independently obtained from a cosmid clone previously mapped to the centromeric region of human chromosome 8. Southern blot hybridization analyses suggested that both subclones contain repetitive DNA sequences different from the chromosome 8 specific alphoid DNA. DNA sequence analysis of the 704 bp insert of 50E1 and the 1,962 bp insert of 50E4 revealed that both inserts contained tandemly repeated units of approximately 220 bp. Fluorescence in situ hybridization studies confirmed these two subclones to be specifically located on the centromeric region of chromosome 8. A 220 bp consensus sequence, derived from nine monomeric repeats, showed no significant homology to alphoid consensus sequences or to other currently known human centromeric DNA sequence. Furthermore, no significant homology was found with any other DNA sequence deposited in the EMBL or GenBank databases, indicating that this chromosome 8 specific repetitive DNA sequence is novel. From slot blot experiments it was estimated that 0.013% of the human genome comprises 1,750 of these monomeric repeats, residing on the centromeric region of chromosome 8 in tandem array(s).

Base Sequence↗