An antigen of rat parvovirus (Kilham virus) was developed for the diagnosis of viral infection in rat colonies by using hemagglutination inhibition (HAI) test. Primary cell cultures from rat embryos were infected with Kilham rat virus. Infected cells obtained at different time post infection were scraped, centrifuged, concentrated one hundred times, sonicated and centrifuged again. The supernatants obtained were titrated by hemagglutination. The specificity was confirmed with positive and negative reference sera. Ninety eight serum samples were studied by using HAI test. The results coincided with those obtained in a reference laboratory. Kilham rat parvovirus antigen obtained from 5 days-infected-cells was specific, sensitive, easy to prepare, with a high yield and it is useful to detect this virus in experimental and production rat colonies.

Animals↗

PubMedDate not supplied

[Epidemiologic data on breast feeding in Florence and the province. Survey carried out in 1364 children born between 1985 and 1987].

We have studied the breast-feeding frequency in Florentine Area. We investigate a group of 1364 children born between January 1985 and June 1987. We collected following data: 1) Kind of feeding at birth. 2) Duration of breast-feeding related with birth-weight, kind of delivery, mother's age, mother's working activity. 3) Growth in relation to feeding practices from birth to 3rd month. The percentage of infants breast-feed in the hospital was 81.6%, 50.5% at the end of third month, 21.9% at the end of sixth month. We have found that children weighing more than 3000 g at birth had an higher frequency of breast-feeding at birth and last longer than children weighing less than 3000 g (p less than 0.01). Maternal age had a clear effect on both the incidence and the duration of breast-feeding. Mother aged 30 years or more begin to breast-feed in lower percentage but they continue breast-feeding for a longer time. One of the factors that has a negative influence on breast-feeding at birth is caesarean section since it precludes early mother-infant contact and early initiation of breast-feeding. Mother's resume working has a negative influence on breast-feeding duration. With regard to growth in first three months of life related with different kind of feeding we have found no differences between breast-feeding and artificial-fed infants.

Age Factors↗

PubMedDate not supplied

[Anomalous tubercle of the human permanent lower central incisor].

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Dentistry↗

PubMedDate not supplied

[Analysis of the mixed dentition].

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Dentition, Mixed↗

PubMedDate not supplied

[Empyema of the gallbladder in an 8-year old boy].

A case of empyema of the gallbladder in a eight year old child is reported. The child presented simptoms relatively constant in the hydrops of the gallbladder, nomely abdominal pain usually confined to the right upper quadrant and or epigastrium, fever, nausea, vomit, dehidratation; a tender abdominal mass was palpable. As usually occurs in childhood, the acute distension of the gallbladder followed in this report, to preceding focus of infection (angina and cervical lymphadenopathy). Ultrasonography permitted to address the correct diagnosis. This was later confirmed at surgery and from histopathologic study.

Child↗

PubMedDate not supplied

[Acute fetofetal transfusion in dichorionic twins: a clinical case report].

Fetofetal transfusion is due to the twin to twin blood shunt caused by vascular anastomoses. It has been suggested that two types of FFT exist: a chronic form existing during pregnancy, and an acute form occurring only during parturition. Cross-circulation may be demonstrated in monochorionic as well as dichorionic placentas. However, such communications were found in 85-100% of monochorionic placentas, compared with one of 68 dichorionic fused placentas. Authors describe one case of acute feto-fetal transfusion in dichorionic twins.

Acute Disease↗