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Biomedical subjects

C Castro

Publications and source records attributed to C Castro.

At least 91 records · Page 5Linked to original sources
PubMed1996

Laparoscopic treatment of esophageal achalasia.

An observational cohort study evaluated the initial results of using laparoscopic-approach cardioesophageal myotomy with Dor-type anterior fundoplicature for esophageal achalasia. The study involved our first 12 patients: five men and seven women whose median age was 51 years. Esophageal motility was vigorous in four patients; the other eight had aperistalsis. Conversion to laparotomy was required in one case. No postoperative mortality occurred. Postoperative complications included one left subdiaphragmatic abscess secondary to perforation of the esophageal mucosa, which was sutured. Median postoperative hospital stay was 5 days (confidence interval, 4.7-6.7 days). Symptom relief (disappearance of dysphagia) was recorded in 10 cases; relief with partial persistence of dysphagia was observed in the remaining two patients, who were treated by postoperative dilatation. As to the postoperative manometric results, the median basal pressure of the lower esophageal sphincter was reduced from 26.3 mm Hg preoperatively to 15.5 mm Hg postoperatively, with a tendency toward statistical significance (p = 0.08); the median esophageal isotopic retention after 15 min decreased from 60% preoperatively to 24.5% after surgery, with a tendency toward statistical significance (p = 0.07). Cardiomyotomy with Dor fundoplicature through a laparoscopic approach seems effective in treating esophageal achalasia.

Esophageal Achalasia↗
PubMed1996

[Ultrasonographic (intra- and extraluminal) pattern of cervico-urethral anastomosis after radical prostatectomy].

Thirteen patients, mean age 61 years (range 54 to 71), diagnosed with prostate adenocarcinoma underwent radical prostatectomy. To achieve a diagnosis they were all performed blood PSA determination, digital rectal examination, transrectal ultrasound and a prostate ultrasound-guided biopsy by automatic gun puncture. Serum PSA quantification, digital rectal examination and transrectal ultrasound were repeated in the immediate post-operative, and three and six months after surgery. Through the information obtained with endocavitary ultrasound, the lumen of the cervicourethral anastomosis and the perianastomotic tissue was assessed. Transrectal ultrasound plays a relevant role in the follow-up of patients undergoing radical prostatectomy. When a patient with prostate radical surgery shows an elevation of serum PSA and/or abnormal digital rectal examination, an ultrasound-guided biopsy (at the node or perianastomotic area) is indicated.

Adenocarcinoma↗
PubMed1996

[Ureterosigmoidostomy detubularized with surgical staplers: C.F.C. (Original technique)].

Description of our modification of the detubulized ureterosigmoidostomy surgical technique (Castiñeiras-Ferragut-Camacho). In an attempt to shorten the surgery time, we used for these changes absorbable mechanical sutures (Poli Gia and T.A.). This paper analyzes the technical details of interest as well as the anatomicosurgical considerations that should be known prior to carry out this type of by-pass. The anatomicophysiological principles on which our developments are based are those referred by N.G. Kock15 and used by R. Hohenfellner and R. Fisch14, when they described the detubulized ureterosigmoidostomy performed manually. We believe that both surgical procedures, R. Hohenfellner & R. Fisch14 and ours (detubulized ureterosigmoidostomy, C.F.C.), are suitable alternatives to conventional ureterosigmoidostomy.

Colon, Sigmoid↗
PubMed1995

Sequence and functional analysis of the Streptomyces phaeochromogenes plasmid pJV1 reveals a modular organization of Streptomyces plasmids that replicate by rolling circle.

pJV1 is an 11 kb, high-copy-number conjugative Streptomyces phaeochromogenes plasmid that replicates by the rolling circle mechanism (RCR). Sequencing combined with functional analysis of deletion, insertion and frameshift mutations was used to characterize the genes involved in plasmid transfer and chromosome mobilization (Cma), the single-strand origin for RCR and an associated strong incompatibility (Sti) determinant. pJV1 contains two essential transfer genes whose expression is regulated by an adjacent repressor gene with similarity to the GntR family of regulators. A consensus sequence specific for the helix-turn-helix motifs of repressor proteins of Streptomyces plasmids is proposed. Unregulated expression of the transfer genes by inactivation of the repressor is lethal. Three additional genes increase intramycelial plasmid spread resulting in pock formation but, unlike the essential transfer genes, are not required for Cma. The pJV1 transfer genes and their regulatory region, but not the minimal replication region encoding the double-strand replication origin and replication protein, are similar in their sequence and arrangement to those of the Streptomyces nigrifaciens plasmid pSN22, revealing a modular organization of Streptomyces RCR plasmids.

Amino Acid Sequence↗
PubMed1995

Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.

Papulacandin B, an antifungal agent that interferes with the synthesis of yeast cell wall (1,3)beta-D-glucan, was used to isolate resistant mutants in Schizosaccharomyces pombe and Saccharomyces cerevisiae. The resistance to papulacandin B always segregated as a recessive character that defines a single complementation group in both yeasts (pbr1+ and PBR1, respectively). Determination of several kinetic parameters of (1,3)beta-D-glucan synthase activity revealed no differences between S. pombe wild-type and pbr1 mutant strains except in the 50% inhibitory concentration for papulacandin B of the synthases (about a 50-fold increase in mutant activity). Inactivation of the synthase activity of both yeasts after in vivo treatment with the antifungal agent showed that mutant synthases were more resistant than the corresponding wild-type ones. Detergent dissociation of the S. pombe synthase into soluble and particulate fractions and subsequent reconstitution indicated that the resistance character of pbr1 mutants resides in the particulate fraction of the enzyme. Cloning and sequencing of PBR1 from S. cerevisiae revealed a gene identical to others recently reported (FKS1, ETG1, CWH53, and CND1). Its disruption leads to reduced levels of both (1,3)beta-D-glucan synthase activity and the alkali-insoluble cell wall fraction. Transformants containing the PBR1 gene reverse the defect in (1,3)beta-D-glucan synthase. It is concluded that Pbr1p is probably part of the (1,3)beta-D-glucan synthase complex.

Aminoglycosides↗
PubMed1995

[Digestive hemorrhage and hemoperitoneum as complications of visceral aneurysms].

Two cases of visceral aneurysm are reported, one of the splenic artery and another of the gastroepiploic artery. The reason for the publication is the rarity, especially of aneurysms of the gastroepiploic artery, and because of the unusual presentations as upper gastrointestinal hemorrhage and hemoperitoneum respectively. The splenic artery aneurysm was diagnosed by CAT scan and the gastroepiploic one during emergency laparotomy because of the hemodynamic disturbance.

Aged↗
PubMed1995

[Paratesticular neoplasms of mesenchymatous origin. Clinico-pathologic study].

There is a group of malignant intrascrotal tumours which represent a very small number when compared to testicular neoplasias and which are known as paratesticular neoplasias. More than 90% belong to the sarcomatous species. From a clinical point of view and based on the patient's age, two groups can be distinguished: sarcomas affecting children and adolescents (primarily rabdomiosarcomas) and those occurring in adulthood, the more frequent histopathological variants being fibrosarcoma, leiomiosarcoma and liposarcoma. This work contributes five paratesticular sarcomas (two rabdomiosarcomas, one liposarcoma, one leiomiosarcoma and one fibrosarcoma). The features related to histogenesis, diagnosis (revealing signs and clinical examination), natural history (dissemination routes based on histopathology) and treatment are reported. With regard to treatment, both the local surgery (total orchiectomy by inguinoscrotal approach with or without hemiscrotectomy), and indications for lymphadenectomy and radiotherapy in relation to staging are discussed though insisting on the chemotherapy approach.

Adolescent↗
PubMed1995

[Clinical features, diagnosis, and treatment of tumor lesions arising in the adrenal gland].

Between 1981 and 1993, 14 patients with tumoral adrenal disease were diagnosed and treated in the Urology Services of both Hospitals. Nature of the adrenal disease was functional in 8 patients (two carcinomas and six pheochromocytomas) and non-functional in the rest (three carcinomas, two adenomas and one myelolipoma). Reference is made to the clinical manifestations and laboratory tests related to hormonal activity, depending on whether the tumour is functional or non-functional, chromaffin or non-chromaffin. Patients with functional carcinomas had Cushing's syndrome, with very clear virtilization signs. Urine 17-hydroxycorticosteroid, 17-cetosteroides and cortisol were all increased, same as plasma cortisol. Patients with pheochromocytomas had hypertension and headaches (six patients), sweating (five patients), anxiety (four patients) and loss of weight (two patients). All of them had increased urine vainillylmandelic acid and catecholamines. Clinical signs and symptoms of non-functional tumours were related to bulk growth and size (in the three carcinomas), and sometimes was highly anodyne, or even absent (in the two adenomas and the myelolipoma), the cause of discovery being accidental during an ultrasound examination. An analysis is made of the different imaging diagnostic procedures performed, such as IVU (performed in 13 patients) with a 38.4% resolutory power; ultrasound (performed in 11 patients) with an 81.8% resolution; CAT (performed in 6 patients) with a 100% resolution capacity and arteriography (performed in 3 patients) with a 100% resolution power, although patients had previously undergone ultrasound and CAT.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Gland Neoplasms↗
PubMed1995

[Nutritional status of school children in poverty conditions from urban and rural areas. Metropolitan region. Chile. 1986-1987].

The aim of this study was to compare the nutritional status of poor children from urban and rural areas and to quantify the impact of socioeconomic, sociocultural and family variables on nutritional status. Weight/age, height/age, weight/height and head circumference percentages were measured in a representative sample of 4509 school children, 39% belonging to a low socioeconomic status and living in the Metropolitan region, chosen according to grade, type of school, sex and geographic area. Children coming from rural areas had significantly higher percentages of undernutrition than children from urban areas according to weight/age (47 vs 34%, and weight/height (7.7 vs 4.6%); likewise they had a higher proportion of height/age ratios below 90% (10.3 vs 5.2%). Head circumference was below 100% in 77 and 65% of rural and urban children. Brachial anthropometric variables were also lower in rural children. The number of siblings and family size were the independent variables with the greatest explanatory power for weight/age and height/age variations. Mother's instruction in urban areas and crowding, family alcoholism and mother's instruction in rural areas, were the independent variables with the greatest explanatory power for head circumference variation. It is concluded that the significant relationship found between socioeconomic, sociocultural and family variables ad nutritional status is relevant, considering that the sample was homogeneous in each geographic area.

Adolescent↗
PubMed1995

[Head circumference in Chilean school children 5 to 18 years of age and socioeconomic status].

The objectives of this study were to determine the head circumference (HC) values of a representative sample of 4,346 school children aged 5 to 18 years from Chile's Metropolitan Region, to carry out a comparative study with international reference data of Tanner, Nellhaus and Roche et al and to compare the HC values by socioeconomic status (SES). The sample included school children of both sexes, from public, private subsidized and private non-subsidized schools and from urban and rural areas. HC was measured according to Jelliffe norms and SES through Graffar's Modified Method. Results showed a high correlation between HC values of school-age children from Chile's Metropolitan Region and Tanner, Nellhaus and Roche et al Tables, with similar growth curves; from this manner, HC values of school-age children from Chile's Metropolitan Region not differ significantly from international reference data. HC values were, in general, significantly higher in males than females in all age groups, in students from high SES to compare with other socioeconomic strata and in older school children. This fact was tested too in 1992, in a representative sample of 605 poor school children beneficiaries from the School Feeding Program in Peñalolen and Pirque Counties from Chile's Metropolitan Region with similar results. It can be concluded that HC values of school-age children from Chile's Metropolitan Region were higher in males than females, in high SES compared with medium and low SES and in older students and does not differ significantly from international reference data.

Adolescent↗
PubMed1994

Sequence of the Streptomyces albus G lipase-encoding gene reveals the presence of a prokaryotic lipase family.

An extracellular lipase (Lip)-encoding gene from Streptomyces albus G has been cloned and sequenced. It encodes a Lip with 82% sequence identity to another previously cloned Lip from a Streptomyces species not closely related. These two sequences can be aligned with 33% identity to the sequence of Lip1 from the antarctic psychrotroph Moraxella TA144 [G. Feller et al., Nucleic Acids Res. 18 (1990) 6431]. An alignment of the three sequences revealed amino-acid substitutions which might be responsible for the greater thermal stability of the Streptomyces lipases. The presence of this lip gene family in several members of the Streptomyces genus was also shown.

Amino Acid Sequence↗
PubMed1994

Reduced oxygen consumption of brown adipocytes isolated from progesterone-treated rats.

It has been previously shown that responsiveness to noradrenaline is reduced in brown adipocytes isolated from estradiol-treated rats. The possibility that high plasma levels of progesterone could also alter adrenergic response was checked. The oxygen consumption of brown adipocytes isolated from control and progesterone-treated rats was monitored in basal conditions and in the presence of increasing concentrations of noradrenaline. In both situations, cells isolated from treated animals showed a lower respiratory rate than those from control animals. These results suggest that not only estradiol but also progesterone could modulate the adrenergic response of brown adipocytes. The study of alpha 1- and beta-adrenergic responses indicates that the beta-response parallels the general reduction in oxygen consumption, although the alpha 1-response seems to be more deeply depressed. Estimation of cell number in brown fat depots indicates some hyperplasia induced by progesterone; this increase in cell number could counterbalance partially but not totally the decreased cellular oxygen consumption at the organ level.

Adipocytes↗
PubMed1991

[A mortality study in the Internal Medicine Emergency Service of October 12 Hospital during 1989].

152,902 patients were attended at the Emergency Department of "12 de Octubre" Hospital during 1989; 48,701 of them were seen in the Internal Medicine area. 196 dead, 164 case histories were selected in order to fulfil the protocol. Several variants were analysed, such as age, sex, diagnosis, time spent at the hospital, quality of life, date, etc. 53.6% were male; the median age was 73 years and the overall median age of all patients seen was 55.9 years. Median Karnovsky was 52.50% of the patients died during their first 5 hours at the Emergency Department. The most frequent diagnoses for the area were neurological, followed by cardiovascular, oncological, digestive, etc. The most frequent diseases observed were cerebrovascular accident, cardiac insufficiency and acute infarction, oncological cases and death on arrival. Mortality was higher in winter. On the whole, mortality was higher in oncology patients followed way behind by neurology digestive and cardiovascular patients.

Age Factors↗