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C Coté

Publications and source records attributed to C Coté.

8 recordsLinked to original sources
PubMed1992

A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry.

The application of immunohistochemistry to routinely decalcified, celloidin-embedded human temporal bone sections has been hampered because of antigen loss during processing of the specimens. To our knowledge, there has been no published report to date describing immunohistochemical staining of such tissues suitable for examination by light microscopy. Here we report a novel antigen retrieval technique which can be successfully used to stain a variety of antigens in routinely formalin-fixed, trichloroacetic acid-decalcified, celloidin-embedded human temporal bone sections. The new procedure reported here for decalcified human temporal bone tissues simply requires immersing slides for 30 min at room temperature in an antigen retrieval solution. A total of 60 decalcified, celloidin-embedded human temporal bone tissues were tested with monoclonal antibodies (MAb) to 15 different antigens. Of these, 12 MAb showed definite positive staining, while three were negative. This technique may prove very useful in studying the expression of various antigens by immunohistochemistry in formalin-fixed, acid-decalcified, celloidin-embedded tissues.

Antibodies, Monoclonal↗
PubMed1992

S-100 protein in human inner ear: use of a novel immunohistochemical technique on routinely processed, celloidin-embedded human temporal bone sections.

The authors recently developed a new antigen retrieval technique which allows immunohistochemical detection of proteins in routinely processed, celloidin-embedded human temporal bone sections. This technique was used in the present study, which reports the occurrence of S-100 protein in the human inner ear. Fifteen celloidin-embedded human temporal bone sections, obtained from the Eastern Temporal Bone Bank at the Massachusetts Eye and Ear Infirmary, were tested with the monoclonal antibody to S-100. This protein was found in the spiral ligament, in Reissner's membrane, in the spiral limbus, and in the basement membrane of the cochlea. S-100-positive, thin fibers could be seen as supporting elements in the acoustic nerve and the facial nerve. This protein was localized along the surface of vestibular wall. The new technique provides an immunohistochemical approach to studying routinely processed human temporal bone sections and may prove useful in the field of immuno-otopathology.

Collodion↗
PubMed1978

Embryonic stimulation of ovine and bovine corpora lutea.

Preimplantation embryos, recovered from sheep on Days 13-15 of pregnancy, were incubated with luteal tissue from cyclic ewes. Extracts of ovine embryos were incubated with luteal tissue from cyclic Holstein cows. Embryos and embryo extracts significantly increased progesterone production by ovine and bovine luteal tissue in vitro. The embryos themselves did not produce measurable amounts of progesterone. We suggest that the ovine preimplantation embryo produces a substance with luteotrophic properties which contributes to the maintenance of early pregnancy.

Animals↗
PubMedDate not supplied

[Physico-chemical properties of ursodeoxycholic acid and its usefulness in hepatopathies].

The authors of this article reviewed the physico-chemical properties of bile acids, with particular attention to ursodeoxycholic acid. Based on this information they explain the rational for treating some chronic liver diseases, like primary biliary cirrhosis and primary sclerosing cholangitis with bile acids. Finally they discussed the results of the different clinical trials of ursodeoxycholic acid in chronic liver diseases.

Bile Acids and Salts↗
PubMedDate not supplied

[Serological markers of viral hepatitis].

In the last three decades we have reinforced our knowledge of the biology, natural history and serology of viral hepatitis. The available serologic tests allow us to determine the state of the disease (acute or chronic), the replicative state of the virus and in some cases to predict prognosis. Serologic assays are technically easy and fast, and generally do not represent any interpretation problem. Advances in molecular biology has permitted the discovery of mutants and new serotypes, as well as the elaboration of assays capable of detecting genomic amounts of specific nucleic acids for further characterization of the hepatitis viruses. This means we are, again, at the beginning of a new area.

Acute Disease↗