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C Dubos

Publications and source records attributed to C Dubos.

15 recordsLinked to original sources

Drought differentially affects expression of a PR-10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings.

A full length cDNA encoding a PR-10 protein was isolated from maritime pine drought-stressed seedlings. The predicted protein contained 150 amino acids, has a molecular mass of 16.7 kDa and an isoelectric point of 5.28. The transcript level of PR-10 displayed a transient accumulation in needles of drought-stressed plants, and was not detectable in root and stem tissues.

Amino Acid Sequence↗

[Chronic obstructive bronchopneumopathy after acute inhalation of sulfur anhydride. Apropos of a case].

We report on an acute accidental inhalation of sulfurous anhydride, by a man aged thirty in the course of his work. This intoxication, followed by acute respiratory distress, first showed an improvement, then re-aggravation 26 days after the accident. This episode has not been explained. The patient then developed a chronic obstructive bronchopathy. Ten years of regular observation allows us to maintain that the severe obstructive syndrome which the patient presents has never regressed (FEV1/FVC = 32%), and must be associated with a chronic toxic obstructive bronchopneumopathy.

Accidents, Occupational↗

[Myocardial infarction and vinorelbine. Report of a case].

The authors report a case of a patient dying as a result of a myocardial infarction which was probably related to the administration of vinorelbine, the vinca alkaloid recently introduced into the treatment of non small cell bronchial cancers.

Antineoplastic Agents↗

Localization of gamma-rays induced chromatid breaks using a three consecutive staining technique.

Three staining techniques (Giemsa, Q-banding and R-banding) are used consecutively to localize the breakage points in chromosomes of human lymphocytes, irradiated during G2-phase with gamma-rays, at doses ranging from 50 to 200 rad. The large majority, about 85% of the breaks, occurs at the interbands, between R- and Q-bands. The discrepancy of this result, with regard to previously reported ones, is attributed to the strong bias of analysis when only one staining technique is used.

Azure Stains↗

Separation and characterization of needle and xylem maritime pine proteins.

Two-dimensional gel electrophoresis (2-DE) and image analysis are currently used for proteome analysis in maritime pine (Pinus pinaster Ait.). This study presents a database of expressed proteins extracted from needles and xylem, two important tissues for growth and wood formation. Electrophoresis was carried out by isoelectric focusing (IEF) in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second. Silver staining made it possible to detect an average of 900 and 600 spots on 2-DE gels from needles and xylem, respectively. A total of 28 xylem and 35 needle proteins were characterized by internal peptide microsequencing. Out of these 63 proteins, 57 (90%) could be identified based on amino acid similarity with known proteins, of which 24 (42%) have already been described in conifers. Overall comparison of both tissues indicated that 29% and 36% of the spots were specific to xylem and needles, respectively, while the other spots were of identical molecular weight and isoelectric point. The homology of spot location in 2-DE patterns was further validated by sequence analysis of proteins present in both tissues. A proteomic database of maritime pine is accessible on the internet (http://www.pierroton.inra.fr/genetics/2D/).

Acrylic Resins↗