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PubMed · 340939

Localization of gamma-rays induced chromatid breaks using a three consecutive staining technique.

C Dubos·E V Pequignot·B Dutrillaux
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Abstract

Three staining techniques (Giemsa, Q-banding and R-banding) are used consecutively to localize the breakage points in chromosomes of human lymphocytes, irradiated during G2-phase with gamma-rays, at doses ranging from 50 to 200 rad. The large majority, about 85% of the breaks, occurs at the interbands, between R- and Q-bands. The discrepancy of this result, with regard to previously reported ones, is attributed to the strong bias of analysis when only one staining technique is used.

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Azure StainsChromatidsChromosome AberrationsCytological TechniquesGamma RaysQuinacrineJournal Article

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C Dubos, E V Pequignot, B Dutrillaux. 1978. Localization of gamma-rays induced chromatid breaks using a three consecutive staining technique.. https://doi.org/10.1016/0027-5107(78)90084-2

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