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Biomedical subjects

C Fava

Publications and source records attributed to C Fava.

At least 37 records · Page 2Linked to original sources

Evaluation of a rapid method to exclude the presence of certain enteric pathogens in stool specimens.

A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid SST. The strip consists of five pairs of cupules for the screening of five lactose-negative colonies. The first cupule of each pair detects the presence of five enzymatic activities, whereas the second serves to maintain the strain for additional testing if necessary. A total of 197 fresh isolates from stool specimens and 217 stock cultures of Salmonella spp., Shigella spp., and Yersinia enterocolitica were tested, with the API 20E system as a reference method. In the stool specimens, 77.3% of the bacteria could be excluded from further workup for the presence of these organisms within 2 h. Over 97% of the stock strains and each of three fresh Salmonella isolates tested produced a reaction pattern corresponding to a correct presumptive identification. This reaction pattern was not produced by any isolate other than the Salmonella isolates. The API Z system can be used as a screen for the presence of Salmonella and Shigella spp. and can provide an accurate presumptive identification of Salmonella isolates within 2 h after primary isolation.

Bacteria

In vitro susceptibility of Salmonella strains to cefotetan and other cephalosporins.

The in vitro activity of cefotetan, a recently developed cephamycin, is investigated by Minimum Inhibitory Concentration (MIC) and by the agar diffusion method against 212 strains of Salmonella (36 species). Cefotetan shows good activity against all strains of Salmonella tested and it is more active than other cephalosporins, in particular, on S. wien.

Cefotetan

Enzyme-linked immunosorbent assay (ELISA) for streptokinase antibodies.

A micro plate Enzyme-linked immunosorbent assay (ELISA), developed for detection of antibodies to Streptokinase, was used to analyze 80 human sera. The aim was to provide a simple method for antibody screening that required neither sophisticated equipment nor a high degree of technological skill. Preliminary results show that ELISA is specific, reproducible and sensitive. Comparative evaluation of the ELISA and the coagulum lysis test for antibodies against streptokinase show excellent correlation. We also evaluated the anti-Streptolysin O titer either by conventional method or ELISA. The sensitivity of ELISA for detecting anti-Streptolysin O antibodies was 3-4-fold higher than that of the hemolytic test. The sensitivity of ELISA for detecting anti-streptokinase antibodies was 10-20-fold higher than that of the coagulum lysis. The Enzyme-linked immunosorbent assay seems to offer some advantages over the more commonly used coagulum lysis test as far as sensitivity is concerned and potentially it is suggested that this method could be usefully employed clinically in human streptococcal infections.

Adult

[Radiological aspects of viral respiratory diseases].

The importance of radiological examination in viral diseases of the respiratory apparatus depends on the site and the type of alterations caused, since the evidence of damage it provides is closely linked to the anatomical and pathological of such alterations. In the case of the upper airways, trachea, and large bronchi, little radiological evidence of the mostly mucosal lesions will be obtained, except in laryngeal forms in children with considerable oedema. Generally speaking, the chances of demonstration are greater in diseases affecting the lung parenchyma, with direct or indirect ventilation changes. Interstitial involvement, which is characteristic of such diseases, will be less easy to demonstrate, especially in the initial stages, on account of the absence of distinct gross evidence of the alteration, irrespective of the clinical picture.

Humans

[Radiological aspects of viral respiratory diseases in adults].

Adults present few radiological signs of damage to the air ways caused by viral agents whereas the importance of such study is much greater in lung pathology, particularly in the course of pneumonia and bronchopneumonia. Typical of this group of disease is the involvement of the interstice which is normally great enough to be detected in X-rays. The different causal agents generally present in their own preferential ways, but specific diagnosis using X-rays signs alone is in practice impossible. At best, some suspicion might be advanced regarding a non-bacterial form as the notable morphological resemblances in fact prevent differential diagnosis among the different virosis forms as well as their distinction from pneumopathies due to Mycoplasma pneumoniae.

Adult

[Post-traumatic pulmonary hematoma].

Pulmonary haematoma is relatively rare. Though on the increase as a consequence of a rise in the number of road accidents, it is not necessarily traumatic in origin. Three cases (two post-traumatic, out of 250 serious chest trauma cases examined) observed at the University of Turin chest and lung surgery centre over the last three years are presented. Data from the literature are also cited. A description is given of the typical radiological features: roundish opacity with distinct edges, homogeneous, in the site the pulmonary or subpleural ligament, slow involution. This picture may be sufficient for diagnosis when the history is not clear.

Adult

[Abnormal pulmonary vein outlet. Radiological aspects (author's transl)].

6 cases of abnormal pulmonary venous outlet, 5 of them partial anomalies without cardiocirculatory symptoms, are reported. Diagnosis was formulated by means of angiocardiography resorted to on the basis of the radiographic picture: retraction of the right hemithorax (left in 1 case), heart attraction, pulmonary veiling, tomographic pictures of anomalous vessels.

Adolescent