Biomedical subjects
Publications and source records attributed to C Osorio.
Serum levels of testosterone, FSH, LH and prolactin were measured by RIA in patients with BPH (benign prostatic hypertrophy) and in control men. The testosterone concentrations in the BPH group were significantly lower than that of the control group. Serum concentrations of FSH, LH, prolactin and estradiol, did not differ significantly in the 2 groups.
The rabbit heteroantisera anti-Meth A sarcoma (H-2d) (RAMA) and rabbit anti-RBL-5 leukemia (H-2b) (RAR-5) have been used to study the presence of normal strain antigens on the surface of different murine tumour cells. RAR-5 detected H-2-like structures on TLX9 (H-bb) lymphoma, however RAMA did not on LSTRA leukemia (H-2d) mastocytoma. Neither RAR-5 nor RAMA contained antibodies against non-H-2 and differentiation antigens.
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The metabolic effects of human placental lactogen (HPL) on rat and human white fat were tested in vitro. When tested against rat tissue, HPL resembled insulin in stimulating uptake of glucose and incorporation of [14C] glucose into CO2, triglyceride and glycogen, but differed from insulin in stimulating glycerol release and in failing to stimulate the incorporation of [14C] The stimulation of [14C] glucose incorporation and the inhibition of glycerol release by insulin were antagonized by HPL. The effects of HPL on human white fat resembled those on rat white fat,except that glycerol release was not stimulated in human tissue. The possible role of HPL in causing the diabetogenic stress of pregnancy is discussed in the light of these findings.
The isolated perfused rat pancreas was utilized to characterize the effect of 1.5 mM oleic acid and 1.5 mM octanoic acid on insulin release in response to glucose (4.4, 8.3, 11.1, 16.7 and 27.7 mM). Both oleic and octanoic acids potentiated glucose-induced insulin release without modification of the pattern of secretion. At 4.4 mM glucose, the potentiation of insulin release by fatty acids was clearcut for the first phase but not significant with respect to the total amount of insulin released during the stimulation period. Maximal potentiation was observed at 11.1 mM glucose both with oleic and octanoic acids. At all glucose concentrations, octanoic acid was more effective than oleic acid in the potentiation of the insulin secretion.
In recently isolated human lymphocytes, no effect of insulin on (U-14C) glucose incorporation into CO2, triglycerides and glycogen, was found over a wide range of insulin concentration. The rates of glucose oxidation, synthesis of triglycerides and synthesis of glycogen are much lower than those observed in chicken and rat adipocytes. The authors discard the possibility of using the human lymphocyte as an instrument in the study of the insulin action on normal and altered human physiology.
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Optimum chromatographic parameters for the separation and detection of estrogen TMS derivatives are described. Estrogens are best separated by using a 2 m glass column with 1% SE-30 load; an 8' iso temperature program, 230 degrees C, 3 degrees C/min, 296 degrees C; 24 ml/min nitrogen flow; 33 ml/min hydrogen flow and 1.5 Kg/cm2 air pressure.
The H-2 specificities in two different H-2b tumour cells (TLX/9 and RBL-5) and a fibroblast line L929 (H-2k) have been studied. Tumour cells were passaged intraperitoneally in syngeneic mice and fibroblast was kept in vitro at 36 degrees C. The typing was performed by means of a postlabelling microradioassay for actively growing cells and 28 H-2 alloantisera of restricted specificity defining private H-2K, H-2D and public antigens. Unexpected reactions in disagreement with H-2 genetics were detected in both tumours but not in fibroblast line. The latter shed C-type particles whereas RBL-5 did not. The cytotoxicity of the alloantisera was quantitatively absorbed by normal lymphoid cells, although some extrareactivities were due to other antibodies present in the sera. RBL-5 grew in mice of different H-2 haplotypes with the same intensity as in syngeneic animals.
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Serum levels of testosterone, follicle stimulating hormone and luteinizing hormone have been measured in 216 healthy children (104 boys and 102 girls) divided in 9 groups from newborn to puberty. Testosterone serum concentrations were significantly higher in boys than in girls during the first 4 months after birth. Testosterone values were highest in 1 to 2 month old boys. Serum concentrations of both FSH and LH changed in different patterns in both sexes. During the first 4 years FSH concentrations were significantly higher in girls than in boys. During the first 4 months, however, LH concentrations were higher in boys than in girls.
The effects of orthostatism and furosemide tests on different parameters of renin-angiotensin system have been studied in 14 normal human subjects. Both tests produced a significant increase in plasma renin activity and plasma renin concentration. Plasma substrate concentration was slightly, but significantly, increased on the furosemide test. Plasma aldosterone rose in both tests, while aldosterone urinary excretion decreased after orthostatism and increased after furosemide.
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The concentrations of nine proteins, alpha-1-acid glycoprotein, antitrypsin, prealbumin, transferrin, albumin, IgG, ceruloplasmin, IgA and alpha-2-macroglobulin, have been determined in the serum and CSF of two groups of patients, one control and one experimental, by an immunological method. The experimental group were patients suffering from grand mal epilepsy. The control group showed no detectable neurological disorder. In the group of grand mal epileptics, only prealbumin showed a significant elevation in CSF when compared with the control group. In contrast, the rest of the proteins are decreased with respect to the controls except for alpha 1-acid glycoprotein and transferrin. The results from this study also suggest that something more than an ultrafiltration process dependent upon molecular weight, is important in determining the concentration of some serum proteins in the CSF.
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The capacity of human foetal lymphocytes to respond to PHA and to form E-rosettes have been compared with data from adult individuals. For this purpose a microculture system that uses whole blood and avoids the problems of lymphocyte separation, has been developed. Foetal lymphocytes reached optimal stimulation with lower dosis of PHA (31,2 microgram/ml) as compared with adult cells (125-252 microgram/ml). However their quantitative response (measured by 14C-thymidine uptake) was equal in both groups. In addition, peripheral T cells (E-rosetting cells) reached values of 36.47 +/- 9% in newborn and 49.6 +/- 10% in normal adult controls. These results are discussed as to the status and development of cellular inmunity in human foetus.
A modification to the radiochemical assay method to measure testosterone in serum, that renders unnecessary the extraction of testosterone from a serum sample is described. The serum is heated at 60 degrees C for 1 hour, thereby testosterone binding proteins are destroyed, then radioimmunoassay follows as usual.