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Biomedical subjects

C R Mason

Publications and source records attributed to C R Mason.

18 recordsLinked to original sources
PubMed1995

Single strand conformation analysis of two genes contained within the first intron of the neurofibromatosis type I gene in patients with multiple sclerosis.

Single strand conformation analysis has become the most widely used technique to screen large numbers of DNA samples for unknown mutations which may contribute to genetic susceptibility to disease. The method relies on the electrophoretic migration of a single strand of nucleic acid in a polyacrylamide gel being dependent on its conformation which is in turn dependent on its sequence. We have examined two closely related genes present in the first intron of the neurofibromatosis type I gene--the oligodendrocyte myelin glycoprotein (OMGP) gene and the ecotropic viral integration 2A (EVI2A) gene--in 36 patients with multiple sclerosis (MS) and 36 healthy controls. A single mutation was found in the OMGP gene which resulted in an amino-acid change of glycine to aspartic acid. This occurred in identical proportions (16.6%) in MS patients and controls. Two rare mutations were found in the EVI2A gene, one resulting in an arginine substituting for a glutamine (one control and one patient), the other in the replacement of a glycine with serine (one control only). A third common polymorphism was seen in 5'-untranslated region of the EVI2A gene, with 65% of patients and controls showing a T-->C transition in either a heterozygous or a homozygous form. This makes it unlikely that either of these genes is involved in genetic susceptibility to MS, but regions of these genes outside of the exonic sequences have not been examined.

Adult↗
PubMed1995

Discriminating coherence in spectro-temporal patterns.

This study examined the ability of trained listeners to discriminate coherent components in randomly varying spectral patterns. In each observation interval, the listener was presented with a sequence of bursts of multitone complexes having a fixed number of tones (m) in each burst. In the standard interval, the frequency of each tone in every burst was chosen randomly between 200 and 5000 Hz. In the signal interval, the frequencies of n tones were repeated throughout the burst sequence while the remaining m-n tones were chosen at random. The n tones were coherent in the sense that they were perceived as "sticking together" to form a pattern. The listener's task was to discriminate which burst sequence contained the n components. The results indicated that discrimination improved with increasing n/m, with increasing number of bursts per interval, and declined as the coherent components were increasingly perturbed in frequency. Further, for a fixed value of the ratio n/m discriminability was relatively independent of m. A model incorporating multichannel filtering and an optimum decision rule was reasonably successful in accounting for the experimental results.

Adult↗
PubMed1995

Binaural advantage for sound pattern identification.

Listeners were trained to identify six patterns of eight sequentially presented 48-ms tone bursts. The variation in frequency forming the patterns was confined to a relatively narrow range around the nominal center frequency, which was either 500, 1000, or 3000 Hz, or was selected randomly on each presentation from a range of 450-3300 Hz. Detection (500 and 3000 Hz only) and identification of the six patterns masked by Gaussian noise was measured in two interaural presentation conditions: masker in-phase and signal in-phase (NoSo), and masker in-phase and signal pi rad out-of-phase (NoS pi). The differences in performance in the two interaural presentation conditions for detection and identification are referred to as "masking-level differences" (MLDs) and "identification-level differences" (IDLDs), respectively. At 500 Hz, MLDs and IDLDs were about 11-13 dB. At 3000 Hz, the MLDs and IDLDs were about 1-3 dB. For the random-center-frequency condition, the slopes of the identification-level functions were much shallower for the NoS pi condition than for the NoSo condition so the binaural advantage was large at low signal-to-noise ratios and declined as signal-to-noise ratio increased. This finding was due to the broad frequency range over which the information was distributed and the decline in the binaural advantage with increasing frequency, a conclusion consistent with that reported for the binaural advantage for speech intelligibility. A second experiment demonstrated that MLDs and IDLDs could be manipulated independently: A 500-Hz tone was added to each element of the 3000-Hz patterns. A large MLD was found--due to detection of the 500-Hz tone--while the identification-level functions were determined solely by the high-frequency information, which produced small IDLDs. Finally, the Gaussian noise masker was replaced by an informational masker comprised of eight randomly chosen eight-tone bursts played simultaneously with the signal-pattern elements which were centered at 1000 Hz. Large amounts of informational masking were found for identification. The slopes of the identification-level functions were much shallower than found for the Gaussian noise masker and a relatively small binaural advantage (about 5 dB) was observed.

Adult↗
PubMed1994

Reducing informational masking by sound segregation.

Informational masking was reduced using three stimulus presentation schemes that were intended to perceptually segregate the signal from the masker. The maskers were sets of sinusoids chosen randomly in frequency and intensity on each stimulus interval or, in some conditions, on every masker burst in a series of bursts within intervals. Masker components were excluded from the frequency region surrounding the 1000-Hz signal to minimize the energetic masking. Masked thresholds as great as 60-70 dB above quiet threshold were observed for some subjects in some conditions. It was shown that this informational masking could be reduced as much as 40 dB by: (1) presenting the masker to both ears and signal to one ear; (2) playing different masker samples sequentially in each interval of every trial; or (3) presenting the signal in alternate bursts of multiple, identical masker samples. For the binaural manipulation, informational masking was reduced because the masker and signal were perceived as originating from different interaural locations. In the latter two manipulations, a difference in the spectral or temporal pattern of the signal and masker provided the detection cue. These effects were interpreted as evidence of the importance of perceptual segregation of sounds in noisy listening environments where signal reception is not limited by energetic masking.

Acoustic Stimulation↗
PubMed1992

A new technique for measuring spectral shape discrimination.

A new technique is described for studying the ability of listeners to discriminate between sounds on the basis of spectral shape, a process called "auditory profile analysis." The advantage of the technique is that it reduces the range of the random rove in level necessary to provide a specified limit on the performance which listeners could achieve by "level detection;" that is, by employing a detection strategy based solely on comparisons of stimulus level. Thresholds were measured for the just-discriminable "ripple" (a pattern of alternating intensity increments and decrements) in an equal-amplitude, multitone reference spectrum for a group of normal-hearing listeners. Broadband, high-pass and low-pass filtered conditions were tested. The results indicated that the thresholds obtained using the new technique were well below the lowest level achievable by level detection (referred to as the "level-detection limit") in all conditions using a 20-dB random within-trial rove in overall level. The lowest threshold occurred for the broadband stimulus while the highest threshold was observed for the most extreme high-pass filtered condition. The new technique appears to be well-suited for study of profile analysis in hearing-impaired listeners where stimulus bandwidth and rove range are limited.

Acoustic Stimulation↗
PubMed1991

Evaluation of simple models of auditory profile analysis using random reference spectra.

This article describes further study of the finding reported by Green et al. [J. Acoust. Soc. Am. 73, 639-643 (1983)] and others that, in certain conditions, the threshold of detectability for an intensity increment to the center tone of a multitone reference spectrum decreased as the number of nonsignal tones increased. That result was considered remarkable since critical-band theory would predict that these nonsignal tones, spaced outside the "critical band" containing the signal, would have no effect on or, at most, slightly decrease within-band detectability--and certainly could not account for the result of improved detectability found in the study cited above. Recently, Henn and Turner [J. Acoust. Soc. Am. 88, 126-131 (1990)] were unable to replicate the result described above, concluding that the phenomenon exists only in "limited conditions" and that is "highly individual" in nature. Further, they speculated that the most likely reason for the discrepancy between their study and previous studies was the selection and/or training of the observers. The present study addressed the effects of the amount of subject training on the finding of Green et al. while controlling the potential effects of stimulus order. Specifically, for a group of three "naive" listeners, thresholds were measured for 3-, 7-, and 21-tone inharmonic complexes as a function of the amount of practice in a mixed-block design. In all cases the group mean thresholds decreased as the number of nonsignal tones increased both initially and after extensive practice for both fixed- and roving-level conditions. Thus the effect does not appear to be an artifact of the amount or order of training subjects receive. The possible role of subject sample size and the magnitude of individual differences in obtaining the effect remains an open question. Two hypotheses suggested to account for the improvement in threshold with increasing number of nonsignal tones were evaluated. The hypotheses were represented by simple mathematical models, referred to as the "multiple-comparison" and "pitch-cue" models. The predictions of both models were compared with the results of a series of detection experiments in which the independent variables were the number of nonsignal tones and amount of random, within-trial "amplitude perturbation" [cf. Kidd et al., J. Acoust. Soc. Am. 79, 1045-1053 (1986)] of the nonsignal tones. Neither model, as applied, provided a satisfactory account of the effects of the main variables of number of tones and amount of perturbation.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗
PubMed1989

Effects of optokinetic velocity and medial vestibulo-cerebellum lesions on vestibulo-ocular reflex direction adaptation in the cat.

Adaptive horizontal vestibulo-ocular reflex eye movements in response to vertical (pitch) rotations were produced by exposing three cats to synchronized horizontal optokinetic and vertical vestibular oscillations at 0.25 Hz. The effects of optokinetic stimulus velocity (6-80 degrees/s peak) and nodulo-uvular cerebellum lesions were studied. All optokinetic velocities elicited vestibulo-ocular reflex direction adaptation. though 6 degrees/s stimuli were somewhat less effective. Restricted aspirations of cerebellar vermis lobules IX and X in two cats resulted in reduced but still clearly evident adaptation.

Adaptation, Physiological↗
PubMed1989

Roving-level tone-in-noise detection.

The detectability of tones, or of intensity increments to tones, in bands of random noise was measured for conditions in which the overall level was fixed or was randomly roved from interval to interval of every experimental trial. The purpose of the within-trial rove was to limit the usefulness of a detection strategy based on overall level or level within a single "critical band." At "supracritical" bandwidths, the functions relating masked threshold to noise bandwidth for the roved conditions were similar to those obtained when no rove was employed. At "subcritical" bandwidths, thresholds were higher in some roved conditions, but, for the largest rove, were still lower than would be predicted from arguments based purely on level detection--with one exception. A comparison of observer performance relative to the statistical limits imposed by the roving-level procedure indicated that the traditional critical-band energy-detector model could not account for the results, which are attributed to discrimination based on spectral shape or on waveshape.

Adult↗
PubMed1988

Evidence for sensory-trace comparisons in spectral shape discrimination.

The ability of experienced observers to discriminate changes in the shapes of complex sound spectra was studied for three conditions. In one condition, the reference spectrum or "background" was fixed in spectral shape across each block of trials; in a second condition, the reference spectrum differed in spectral shape from trial to trial within each block of trials but was the same for the two presentations within a trial; and, in a third condition, the reference spectrum differed in spectral shape on every stimulus presentation. The variation in the reference spectrum was a random perturbation in the amplitudes of the tonal components comprising the complex sounds. The signal was an intensity increment to the center component (1000 Hz) of the complex and was present in one interval of each two-interval, two-alternative, forced-choice trial. The principal experimental manipulations were the degree of amplitude perturbation of the reference spectrum and the interval of time between the two stimulus presentations of each trial (interstimulus interval, ISI). The theory proposed by Durlach and Braida [J. Acoust. Soc. Am. 46, 372-383 (1969)] describing memory processes involved in the perception of sound intensity was used to explain the experimental results. As that theory was applied in this study, only the condition in which the stimuli were perturbed between trials showed evidence for comparisons based on "sensory traces," while the conditions in which the stimuli were fixed or were perturbed within trials were best explained by comparisons based on judgments relative to the stimulus context.

Acoustic Stimulation↗
PubMed1987

Level discrimination as a function of level for tones from 0.25 to 16 kHz.

Difference limens for level (delta L in dB = 20 log [(p + delta p)/p], where p is pressure) were measured as a function of level for tones at 0.25, 0.5, 1, 2, 4, 8, 10, 12, 14, and 16 kHz. At each frequency, test levels encompassed the range from near threshold to 95 dB SPL in steps of 10 dB or smaller. The stimulus duration was 500 ms and the interstimulus interval was 250 ms. An adaptive two-alternative forced-choice procedure with feedback was used. Results for six normal listeners show individual differences among listeners, but the general trends seen in the average data clearly are present in the individual data and show the following. First, the delta Ls at all but the highest frequencies are generally smaller at high levels than at low levels. Second, the delta Ls at equal SPLs are largely independent of frequency up to about 4 kHz, but increase with frequency above 4 kHz. Third, at 8 and 10 kHz, the delta Ls are clearly nonmonotonic functions of level, showing consistent deterioration in the mid-level delta Ls relative to the low- and high-level delta Ls. The present data are discussed qualitatively in terms of current models of level discrimination.

Acoustic Stimulation↗
PubMed1986

Auditory profile analysis of irregular sound spectra.

The discrimination of changes in the shapes of sound spectra is reported. The change was always an intensity increment to the 948-Hz component of a multitone complex. First, the ability of naive listeners to learn to discriminate a change in a "regular" background or reference spectrum (equal-level tones equally spaced in logarithmic frequency) was measured as a function of the number of trials. On the average, threshold improved about 10 dB over 3000 trials, with about 50% of the decrease in threshold occurring during the first 750 trials. In a subsequent series of experiments, the overall pattern of spectral shape of the background was varied randomly. Two kinds of perturbations in spectral shape were employed: Randomly choosing the frequencies of the reference spectra and randomly choosing the amplitudes of the components of the reference spectra. The experimental manipulations involved fixing the random spectra across a block of trials, varying the reference spectra from interval to interval of each trial, and providing extensive practice in discriminating specific randomly perturbed reference spectra. The results of the spectrum-learning and random perturbation experiments provide insight into the roles of critical band filtering, sensory variability, and short-term and long-term memory representations in auditory profile analysis. Further, the appropriateness of the generalization of a simple energy detection model is discussed.

Acoustic Stimulation↗
PubMed1985

One method for assessing the effectiveness of fast brushing.

The purposes of this study were to determine if fast brushing of the skin overlying a muscle is effective in increasing the sensitivity of the stretch reflex of that muscle and to detect the optimal rate and duration of brushing to elicit this effect in healthy adults. The stretch sensitivity of the right lateral gastrocnemius muscle was measured by EMG activity and force produced by the abrupt tap of the Achilles tendon before and after fast brushing. Peak-to-peak EMG and force from 10 taps were established. Eight different conditions (two control and six experimental brushing) were applied to 10 healthy adult subjects (age range, 30 to 46 years). The experimental conditions included rotary brushing at 5 Hz, 180 Hz, or 360 Hz for 5 seconds or at 5 Hz, 180 Hz, or 360 Hz for 30 seconds. Postbrushing taps began 30 seconds after the brush was turned off and continued every 5 minutes for 30 minutes. This method of fast brushing did not increase the stretch sensitivity of the muscle in healthy adults. Possible factors influencing the results of the study and recommendations for future studies are presented.

Achilles Tendon↗
PubMed1985

Auditory profile analysis: frequency, phase, and Weber's law.

This paper reports three separate experiments on different aspects of performance in auditory profile analysis. The first experiment deals with the effects of the frequency and position of an increment in a single component of a multitonal complex. The general results indicate that detection of the signal is easier for components in the mid-frequency range (around 1000 Hz) independent of signal position within the complex. The second experiment investigates the effects of relative phase of the individual components of the complex. Regardless of the number of components, our results indicate that phase has very little effect, even when different phases are selected for each presentation. The third experiment compares the detection of an increment in intensity of a single component, the traditional Weber fraction experiment, and conditions where additional components are present, a profile experiment. The detection of the increment is measured as a function of the level of the standard. The single-tone condition shows the usual near miss to Weber's law whereas the multitone condition does not. In addition, threshold for the increment is better for the multitone condition than for the single tone condition for levels of the standard up to 70 dB SPL. This last result is investigated for ten observers, five of whom were experienced in profile tasks and five of whom were not. Using a low level standard, the five experienced observers replicate the results described above. The inexperienced observers show the opposite result. On average, they are better able to detect the increment in the single-component condition.

Auditory Threshold↗
PubMed1984

Profile analysis and level variation.

This study examines the effects of random level variation, a method used in studies of profile analysis [3-6,14,15]. Presentation levels for a complex of sinusoids are varied randomly on each interval of a two-interval, forced-choice detection task in which subjects are required to detect an increment on one of the sinusoidal components of the complex. Three experiments are reported. The first experiment examines the effect of the range of level variation. The second is concerned with the effects of the median level about which the presentation levels vary. The third experiment is designed to provide a within-trial analysis of the effect of the differences in presentation levels. As the range of level variation is increased, ability to detect the increment decreases. The results indicate that detection performance is best at moderate intensity levels and decreases at lower and higher levels. Finally, the difference in levels within a single trial has little if any effect.

Acoustic Stimulation↗
PubMed1984

Temporal integration of forward masking in listeners having sensorineural hearing loss.

Findings are described from three experiments that were designed to contrast estimates of auditory time and frequency analysis in highly practiced, young adult subjects having sensorineural hearing loss of cochlear origin with subjects having normal hearing. In one experiment, pure-tone detection threshold was measured for two durations of tones. For the hearing-impaired subjects, smaller differences were found between thresholds for the two durations of tones than were found for the normally hearing subjects at test frequencies where the sensitivity loss was greatest. In a subsequent forward masking experiment, masked thresholds were obtained at 3 kHz as a function of masker level at several masker durations. The results indicated that, for the range of values tested, masked probe thresholds changed less as a function of masker duration for the hearing-impaired subjects than for normally hearing subjects. However, forward masking grew as a function of masker level more steeply for the hearing-impaired listeners than for the normally hearing listeners. We believe that this result indicates abnormal temporal processing of sequential sounds. In a third experiment, psychophysical tuning curves were measured in a forward masking procedure for two maskers that differed in duration by approximately an order of magnitude. Again, the hearing-impaired subjects demonstrated less change in forward masking as a function of masker duration than normally hearing subjects. Further, the sharpness of the tuning curves from the hearing-impaired listeners was markedly reduced as compared to normal. It is believed that the results reflect a disruption of the normal temporal and spectral representation of sounds in the hearing-impaired subjects.

Auditory Threshold↗
PubMed1984

Profile analysis: critical bands and duration.

The detection of an increment in the intensity of the central component of a multi-component complex was measured as a function of the frequency spacing of the components and the duration of the presentation. The overall intensity of the complex was randomly varied on each presentation of the stimulus. Curiously, the increment becomes easier to hear as the range and density of the surrounding complex is increased. This increase in range and density is also effective in improving the detectability of the increment when there is no random variation in intensity, i.e., a conventional Weber fraction experiment. This is unlike the results obtained in many other critical-band experiments where energy remote from the signal frequency has little or no effect. Measurement of the effects of signal duration showed that when presentations were shorter than about 100 msec a greater increment in intensity was required than for longer durations. These results with duration are similar to those obtained in other intensity-discrimination tasks.

Discrimination Learning↗
PubMed1984

Relation of feedlot performance and certain physiological responses to the metabolizable protein and urea content of cattle diets.

An experiment was conducted to test the accuracy of the metabolizable protein system in predicting the amount of urea that would be useful in a corn-based cattle diet. Treatment diets included a basal, low-protein (7.8% CP) negative control (NC) with no supplemental N and a positive control (PC) that contained soybean meal. Urea was added to the NC diet in quantities calculated to be either 25% deficient (LU), equal to (MU) or 25% in excess (HU) of the urea fermentation potential ( UFP ). In vitro rumen fermentation studies were used to determine sequential ammonia production and digestible dry matter content of the diets. In a growth trial, 12 individually-fed Angus, Hereford and Angus X Hereford steers weighing an average of 213 kg were assigned randomly to each treatment diet. At the conclusion of the 112-d trial, rumen ammonia and jugular blood urea N (BUN) concentrations were determined on two steers from each treatment before feeding and at 1, 2, 3, 4 and 5 h postfeeding. In vitro ammonia concentrations of the NC and PC treatments were lower (P less than .05) than that of urea containing diets. In vivo rumen ammonia concentrations at 1 h postfeeding and BUN levels at 3 h postfeeding were low for both the NC and PC diets compared with urea-containing diets. Both of these values increased with each successive increase of added urea to the NC diet. During the initial 70 d of the growth trial, daily gains were improved (P less than .05) by addition of urea up to the MU level, which fulfilled the calculated UFP .(ABSTRACT TRUNCATED AT 250 WORDS)

Ammonia↗