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Biomedical subjects

C Régnier

Publications and source records attributed to C Régnier.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

Gene transcript assay by real-time RT-PCR in epithelial breast cancer cells selected by laser microdissection.

The cell type heterogeneity within clinical cancer tissue samples may affect the accuracy of gene expression analysis. In order to validate our laser microdissection (LMD) method using the Leica AS LMD system (LEICA Microsystems), we compared the mRNA levels of three major genes involved in breast cancer (ERalpha, PR, HER2), measured by means of real-time quantitative RT-PCR, in 5000 microdissected malignant epithelial cells and in corresponding bulk tumor homogenates from 14 patients. We also compared the mRNA level results to protein expression measured by immunohistochemistry (IHC) on the same tumors. For the three genes, significant correlations were found between mRNA results obtained on microdissected cells and IHC. Comparison between IHC and mRNA results obtained on microdissected cells and bulk tumors showed that in all cases microdissection enhanced the sensitivity of assessing target gene transcript levels and was essential for their accurate evaluation in heterogeneous tumors.

Adult↗
PubMedDate not supplied

[Severe hyperlactacidemia in 2 children treated for malignant tumors. Role of vitamin B1].

Two cases of children treated for malignant tumor and who developed a severe lacticacidosis are reported. A 2 year-old girl and a 8 year-old boy were treated with chemotherapy, irradiation and multiple surgical procedures for nephroblastoma and lymphoma respectively. These two malnourished patients, under exclusive parenteral nutrition for two weeks without vitamin intakes, suddenly developed neurological, cardiovascular and digestive symptoms, associated with cytopenia and lacticacidosis. Injection of vitamin B1 only corrected these abnormalities within a few hours, proving the role of thiamine deficiency as the cause of the symptoms.

Acidosis, Lactic↗
PubMedDate not supplied

[Maternofetal infection by parvovirus associated with antenatal meconium peritonitis].

A case of meconium peritonitis associated with materno-fetal infection by human B19 parvovirus is reported. The meconium peritonitis was recognized by ultrasonography at 25 weeks of pregnancy and was confirmed after delivery. Postnatal "opaque index" of the small bowel revealed multiple stenosis. Evolution was favorable and did not require surgery. Diagnosis of B19 parvovirus materno-foetal infection was documented on specific IgM antibodies and viral DNA in the maternal serum and specific IgM antibodies in the infant's serum. Thus, B19 parvovirus appears to be responsible for non lethal fetopathies.

Antibodies, Viral↗
PubMedDate not supplied

[Acute leukemia in the child: quality of the "cure". Apropos of 41 cases].

The authors studied retrospectively a group of 41 children in prolonged remission of Acute Leukemia (AL) or Non Hodgkin Malignant Lymphoma (NHML) treated with polychemotherapy and prophylactic cranial irradiation, searching for late side effects of the disease or its treatment. The neurological follow-up was satisfying enough (only one major sequela); growth abnormalities were found in 57.5% of children (correlated with hormonal deficiency), and some thyroid and gonadal impairments. Psychoaffective disturbances seemed to be moderate, but difficulties in learning and persistence of anxiety are emphasized. Estimation of cardiac function showed left ventricular alterations in two patients, detected by echocardiography, perhaps related to anthracyclines toxicity. Immunohematological investigations showed an apparent recovery for the first year following the end of the treatment, except for 5 inverted T4/T8 ratios and partial vaccine protection. No liver dysfunction was noticed, although positive HB serological assays were 3 fold more frequent than in a normal population. Lastly, no secondary cancer was observed in this study, but the incidence of a second malignancy in such groups is discussed. With the improvement of prognosis of childhood malignancies, such screenings are indispensable for better management.

Acute Disease↗