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Biomedical subjects

C Witting

Publications and source records attributed to C Witting.

At least 19 recordsLinked to original sources
PubMed1992

Zinc concentrations in human tissues. Liver zinc in carcinoma and severe liver disease.

In 58 unselected necropsies, zinc (Zn) in specimens of liver (mean +/- s = 280 +/- 178 micrograms/g d.w./4.28 +/- 2.72 mumol/g d.w.), kidney (170 +/- 57 micrograms/g d.w./2.60 +/- 0.87 mumol/g d.w.), spleen (69 +/- 13 micrograms/g d.w./1.05 +/- 0.20 mumol/g d.w.), and lung (64 +/- 16 micrograms/g d.w./0.98 +/- 0.24 mumol/g d.w.) were analysed by means of flameless atomic absorption spectrometry. In the examined range from 25 to 92 years of age, the Zn concentrations in all tissues measured did not show age or sex-dependency. There was a high correlation between the tissue concentration of Zn and Mg in lung, liver, and spleen, between Zn, Mg, and Pb in liver as well as between Zn and Pb and Zn and Cd in kidney. All hepatic cirrhosis cases had very low Zn conc. in liver tissue, the tumor cases generally increasing Zn conc. in liver tissue with progressive stage of regional invasion and distant metastasis.

Adult↗
PubMed1979

Incidence of immune complex nephritis following active immunisation with a testosterone-3-BSA conjugate or BSA alone.

Before immunisation with hormones can be applied in therapeutic medicine, the possible immunological side effects should be investigated. Therefore the kidneys of 20 rabbits immunised with testosterone-BSA conjugates were examined 4 to 30 weeks after immunisation for the presence of immune complex precipitation and glomerulonephritis. Eighty-five per cent showed differing degrees of precipitation and 50% had an exudative glomerulonephritis. None of the kidneys from normal control rabbits or those immunised with complete Freund's adjuvant or BSA showed any signs of immune complex precipitation, although high anti-BSA titres were present in the BSA-immunised animals (1/3000--1/25,000). BSA antibodies could not be detected in the rabbits immunised with testosterone-3-BSA. In the light of these findings active immunisation with steroid-BSA conjugates would not be advisable as a means of therapy.

Animals↗
PubMed1978

Effect of generalized graft-versus-host-reaction on B- and T-lymphocytes and a benzpyrene-induced murine sarcoma.

UNLABELLED: The investigations of 400 adult hybrids from A/Jax fem. BALB/c male were aimed at answering the following questions: 1. What is the influence of a generalized graft-versus-host-reaction (GVHR) on the induction and growth of benzpyren-induced sarcoma? 2. What is the behavior of B- and T-lymphocytes during GVHR, and during induction and growth of benzpyren-induced sarcoma? 3. Which are the deductions to be drawn with regard to the effect of B- and T-lymphocytes during tumorigenesis and tumor growth? RESULTS: 1. The number of tumors was significantly greater in the GVHR animals (74.5%) than in the controls (49.5%). 2. A significant decrease of T-lymphocytes and increase of B-lymphocytes during GVHR were found in the peripheral blood. 3. The examination of the tumor marginal zone showed a marked decrease of the total round cell count and the T cells, while the count of B-lymphocytes remained uninfluenced. These results are discussed and compared with results of other authors.

Animals↗
PubMed1978

Effect of splenectomy on B- and T-lymphocytes and a benzpyrene-induced murine sarcoma.

UNLABELLED: The investigation of 400 adult mice had the objective to find out 1. The influence of splenectomy on genesis and growth of a benzpyrene-induced sarcoma and 2. The behavior of B- and T-lymphocytes under the influence of splenectomy. RESULTS: 1. A significantly lower number of tumors developed in splenectomized animals (28.5%) as compared to controls (49.5%). 2. A significant decrease of B-lymphocytes and an increase in T cells after splenectomy were found in the peripheral blood. 3. The examination of the tumor marginal zone showed a decreased number of B-lymphocytes and an increase of T-lymphocytes, while the total count of round cells remained unchanged. These results are discussed and compared with results of other authors.

Animals↗
PubMed1978

Studies on proliferation kinetics in liver and spleen during early graft-vs-host reaction in mice.

Autoradiographic and liquid scintillation counting studies were made during the early graft-versus-host-reaction in mice. At the height of GVHR on the 10th day after transplantation, hepatosplenomegaly correlated with the radioactive disintegrations (DPM). This coincided with a high proliferation rate of the blasts that appeared in the spleen, and of the portal infiltrations and Kupffer cells in the liver. Convenient control groups were used to show that these changes are a specific reaction in GVHR.

Animals↗
PubMed1978

[Clinical treatment of chronic lymphatic leukemia by means of extracorporeal blood irradiation].

A chronic lymphatic leukaemia (CLL) with hepato-splenomegaly was treated in a 76 years old female patient by means of extracorporeal irradiation of blood (ECIB), after which a complete involution of the tumour in the spleen had taken place. Leukocytes initially increased to 35,500/mm3, amounting to 10,700/mm3 later on. The patient died of pneumonia in general cachexia. No signs of CLL could be found macroscopically after autopsy. An involution of the lymphatic infiltration could be identified microscopically in the bone-marrow.

Aged↗
PubMed1977

[Immunofluorescence studies of experimental glomerulonephritis in formalin-fixed and paraffin-embedded material (author's transl)].

Immunofluorescence studies are an essential part of the diagnosis in glomerulonephritis. For these studies unfixed, frozen material was used only, on account of intense background staining of formalin-fixed slides. A method developed by Huang (1975) was tested on experimental glomerulonephritis. He succeeded in minimizing the background staining of formalin-fixed liver tissue with pronase-treatment. Our results show no differences in the immunofluorescence of the experimental glomerulonephritis between frozen and paraffin-embedded sections. It seems to be possible therefore to investigate a glomerulonephritis in formalin-fixed material by this method.

Animals↗
PubMed1977

Ultrastructural and histochemical investigations of spindle-shaped corpuscles in phagocytic cells of lymph nodes.

In connection with various diseases, spindle-shaped or roundish 2-15 micrometer large corpuscles with a yellowish-green, partly brown color are found in phagocytic cells of lymph nodes of various localisation. There are three groups according to staining and ultrastructural characteristics. I. Strongly Fe+++ positive corpucles only, 2. corpuscles with a positive PAS-reaction, Ziehl-Neelsen and Sudan black B stain and slight iron adsorption, 3. particles with irregular stainability. Ultrastructurally, the spindle bodies of Group I have a uniform membrane, double in some sections and a narrow, finely granular rim, in which 50 A large iron micelles are stored. The electrondense center contains structures which may correspond to remainders of cell organelles. The similarly shaped particles of Group 2 have a homogeneous, electron-dense center and sometimes half-moon-shaped, homogeneous, less dense appositions. A dense round center with finely honey-combed translucencies and marginal vacuoles characterizes the irregularly shaped, in part spindly corpuscles of Group 3. The energy-dispersion X-ray analysis indicates only iron for the particles of Group I. A relation of the corpuscles to specific diseases, especially to sarcoidosis and certain therapeutic measures, is not evident.

Humans↗
PubMed1977

Immunofluorescence studies on formalin-fixed and paraffin-embedded material.

In an attempt to apply immunomorphologic techniques to formalin-fixed material, various tissues were studied by indirect immunofluorescence. To minimize nonspecific background staining, formalin-fixed paraffin sections were treated with pronase prior to incubation with the antisera. This technique was published by Huang in 1975 to study hepatitis B core and surface antigen in formalin-fixed liver-tissue. Our examination demonstrate that this technique might enable the morphologist to identify immunoglobulins in formalin-fixed tissues and so to get new criteria for their diagnosis.

Adult↗
PubMed1977

[Gastrointestinal sarcomas].

In 45 primary sarcomas of the gastrointestinal tract the authors attempt to derive criteria for prognosis from histology and localization of the tumors and from clinical parameters. Their observations lead to the conclusion that 1) proximal sarcomas of the gastrointestinal tract have a better prognosis than distal sarcomas, 2) lymphocytic malignant lymphomas has a better prognosis than other histologic types, and 3) there is a correlation between blood sedimentation rate and prognosis.

Adolescent↗
PubMed1976

Immunofluorescence studies on pathologic routine material: application to malignant lymphomas.

In an attempt to apply immunmorphologic techniques to pathologic routine material, malignant lymphomas of the Non-Hodgkin- and the Hodgkin-type were studied by indirect immunofluorescence. To minimize nonspecific background staining formalin fixed paraffin sections were treated with protease prior to incubation with the antisera. The usefullness of this procedure in providing additional diagnostic criteria was tested on specimens of malignant lymphomas (chronic lymphocytic leukemias, immunocytic lymphomas, plasmocytic lymphomas, centrocytic lymphomas, centroblastic-centrocyctic lymphomas, centroblastic lymphomas, immunoblastic lymphomas, lymphoblastic lymphomas of Burkitt, convoluted and unclassified type, Hodgkin's disease). In addition, cases of nonspecific lymphadenitis were studied. The discrimination of single cells particularly of the B-cell line was facilitated using immunofluorescence and different patterns were easily recognized thus aiding the diagnosis based on light microscopic criteria. In addition, preliminary evidence was obtained that lymphoblastic lymphomas categorized as "others" may be further characterized.

Fluorescent Antibody Technique↗
PubMed1976

[Amyloid tumors in soft tissues of the neck].

Tumorous amyloid deposits in the neck region of a 31-year-old man caused difficulties in the preoperative differential diagnosis of a tumorous process of the thyroid gland. Several tumorous amyloid deposits compressing and eroding the left nevus vagus and having no connection to the trachea or thyroid gland were removed. Such isolated tumorous amyloid deposits in the neck region have not been described before. Histologically no thyroid tissue was found. A medullar cancer of the thyorid gland that has to be excluded in such cases was not found.

Adult↗