Biomedical subjects

Christopher Fang-Yen

Publications and source records attributed to Christopher Fang-Yen.

4 recordsLinked to original sources

PubMed2006

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy.

We describe azimuthal light scattering spectroscopy (phi/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles phi = 0 degrees and phi = 90 degrees preferentially isolates the single backscattering contribution due to large (approximately 10 microm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using phi/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

Cell Nucleus↗

PubMed2005

Harmonic phase-dispersion microscope with a Mach-Zehnder interferometer.

Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a Mach-Zehnder geometry as an instrument for imaging biological samples. Several improvements on the earlier design are demonstrated, including a single-pass configuration and acousto-optic modulators for generating the heterodyne signals without mechanical arm scanning. We demonstrate quantitative phase-dispersion images of test structures and biological samples.

Equipment Design↗

PubMed2004

Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry.

We describe a quantitative phase-imaging interferometer in which phase shifting and noise cancellation are performed by an active feedback loop using a reference laser. Depth gating via low-coherence light allows phase measurement from weakly reflecting biological samples. We demonstrate phase images from a test structure and living cells.

Equipment Design↗

PubMed2004

Noncontact measurement of nerve displacement during action potential with a dual-beam low-coherence interferometer.

We have used a novel phase-referenced heterodyne dual-beam low-coherence interferometer to perform what we believe are the first noncontact measurements of surface motion in a nerve bundle during the action potential. Nerve displacements of approximately 5-nm amplitude and approximately 10-ms duration are measured without signal averaging. This interferometer may find general application in measurement of small motion in cells and other weakly scattering samples.

Action Potentials↗