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Biomedical subjects

D Berman

Publications and source records attributed to D Berman.

At least 73 records · Page 4Linked to original sources
PubMed1978

Validity of fecal coliforms, total coliforms, and fecal streptococci as indicators of viruses in chlorinated primary sewage effluents.

Quantities of combined chlorine that usually destroyed more than 99.999% of the indigenous fecal coliforms, total coliforms, and fecal streptococci in primary sewage effluents destroyed only 85 to 99% of the indigenous viruses present. Viruses were recovered from five of eight chlorinated primary effluents from which fecal coliforms were not recovered by standard most-probable-number procedures. The limited volumes of such chlorinated effluents that can be tested for indicator bacteria with currently available multiple-tube and membrane filter techniques restrict the value of fecal coliforms, fecal streptococci, and even total coliforms as indicators of viruses in these effluents. Although fecal coliforms and fecal streptococci are useful indicators of viruses in effluents from which these bacteria are recovered, the absence of these bacteria and even total coliforms from disinfected effluents (in standard tests) does not assure that viruses are also absent.

Chlorine↗
PubMed1978

Unilateral forelimb deafferentation in the monkey: purposive movement.

Monkeys with unilateral dorsal rhizotomy C2-T3 used their denervated limbs to retrieve unseen food pellets when movements of the intact contralateral limb were rendered ineffective by enclosure of the hand within a container. Even after protracted use in this condition, however, the deafferented limb ceased to be used in the test situation when the intact arm was released. These findings cast doubt on interpretations of lack of use of a unilaterally dorsal rhizotomized limb as being due to cross-spinal inhibition or to learned disuse.

Afferent Pathways↗
PubMed1973

Kinetics of reaction of Escherichia coli ribosomal proteins toward N-ethylmaleimide.

The kinetics of reaction of the exposed ribosomal proteins from Escherichia coli toward N-ethylmaleimide (NEM) have been studied. While most of the proteins from the 30S subunit react rapidly with NEM and plateau levels can be reached, most of the proteins from the 50S subunit show biphasic uptake kinetics. Furthermore, differences have been observed in the reactivity of proteins toward NEM depending on whether the protein is in the free subunit or in the 70S ribosome.

Bacterial Proteins↗
PubMed1971

Recovery of small quantities of viruses from clean waters on cellulose nitrate membrane filters.

A method is described for quantitatively recovering small amounts of viruses from large volumes of buffered, distilled water. Development of the method was motivated by the anticipated need for testing large volumes of renovated sewage for viruses. The method consists of adsorbing viruses onto cellulose nitrate membrane filters (0.45 mum pore size) from water containing sufficient Na(2)HPO(4) to produce a molarity of 0.05 and sufficient citric acid to produce a pH of 7, and eluting the adsorbed viruses in 3% beef extract under extended sonic treatment. Complete recovery of poliovirus 1, echovirus 7, and coxsackievirus B3 resulted when less than 100 plaque-forming units were added to 1-liter quantities of water. Recoveries of reovirus 1 were almost as good. Preliminary studies indicate that good recoveries can be made from 25-gal quantities of water. The method described is efficient in waters of high quality and may be useful for recovering viruses in renovated, and perhaps in tap waters, but not in waters containing certain organic matter unless that matter is first removed.

Animals↗