Seasonal variation of microcystin concentration in Lake Chaohu, a shallow subtropical lake in the People's Republic of China.
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Bacterial Toxins↗
Biomedical subjects
D Deng
Publications and source records attributed to D Deng.
Hyperhexosemia induced functional and structural changes in the kidneys: role of endothelins.
BACKGROUND/AIMS: Glomerular basement membrane (GBM) thickening and mesangial matrix expansion are characteristic features of diabetic nephropathy. The present study investigates the role of endothelins (ETs) in the pathogenesis of such changes in diabetic nephropathy. METHODS: Diabetic (streptozotocin-induced, 65 mg/kg), galactose-fed (30%) and control animals were followed up for 1 and 6 months. Animal groups also included diabetic and galactose fed animals on dual ET(A)/ET(B) receptor antagonist bosentan (100 mg/kg). A semi-quantitative reverse transcription polymerase chain reaction method was used to quantify mRNA expression of ET-1, ET-3, ET(A), ET(B), fibronectin and collagen alpha2(IV). Histological analyses of the kidneys and ET-1, ET-3 and fibronectin immunohistochemistry were performed. Morphometric assessment of the GBM after 6 months was performed. RESULTS: Diabetes increased mRNA expression of ET-1, ET-3, ET(A), ET(B), fibronectin and collagen alpha2(IV) after one and six months. In contrast, although increased ET(A) and ET(B) mRNAs were present following galactose feeding both at 1 and 6 months, ET-1, ET-3, fibronectin and collagen alpha2(IV)mRNAs were increased after 6 months. Both diabetes and galactose feeding caused increased GBM thickening. Furthermore, diabetes caused an increase in mesangial matrix production. Bosentan prevented increased fibronectin and collagen alpha2(IV) mRNA expression, increased mesangial matrix deposition and GBM thickening. CONCLUSION: This study has demonstrated that diabetes and galactose feeding induced functional and structural changes in the kidney are mediated via ETs.
Animals↗
Helicobacter spp. infection in cats: evaluation of the humoral immune response and prevalence of gastric Helicobacter spp.
The principal aims of this study were to evaluate the humoral immune response (IgG) of cats with gastric Helicobacter spp. infection, and to determine the prevalence of different types of Helicobacter spp. in the stomachs of cats. The Helicobacter infection status of 45 cats (12 healthy spay/neuter cats, 9 sick cats, 24 colony cats) was determined by evaluating endoscopic gastric biopsies for urease activity, presence of Helicobacter-like organisms (HLO) on histopathology, and genus and species-specific PCR. Serum samples were evaluated with a kinetic enzyme linked immunosorbent assay (ELISA) utilizing the high molecular cell-associated protein (HM-CAP) fraction of H. felis ATCC 49179.Seventeen of 45 cats were infected with Helicobacter spp.: "H. heilmannii" 9/17, H. felis 4/17, mixed "H. heilmannii" and H. felis 3/17, unclassified-Helicobacter spp. 7/17. H. pylori was not detected in any cat. Kinetic ELISA results were significantly higher for infected cats, than for uninfected cats. Cats infected with different Helicobacter spp. showed similar distribution of OD/min values. There were no effects of age or clinical signs on the results of kinetic ELISA. No correlation between colonization density and seroconversion was observed. There were statistically significant, but weak correlations between the degree of seroconversion and the degree of inflammation, and the number of lymphoid follicles. Infected cats had more severe inflammation in the pylorus and fundus than uninfected cats. Infected sick cats had a higher degree of pyloric, but not fundic inflammation, than healthy infected cats and uninfected sick cats. The results indicate that naturally acquired infection with gastric Helicobacter spp. is associated with seroconversion (IgG) in cats. The similar ELISA values in cats infected with a variety of Helicobacter spp. suggests substantial antigenic homology between different Helicobacter spp. The higher degree of inflammation in infected than uninfected cats, supports a role for Helicobacter as a cause of gastritis in cats.
Animals↗
[Diagnosing complicated congenital heart disease with two-dimensional echocardiography and three-segment concept].
OBJECTIVE: To apply three-segment heart anatomy concept to two-dimensional echocardiography (2DE) in the diagnosis of complicated congenital heart disease (CHD). METHODS: 920 cases of CHD were diagnosed with 2DE and three-segment heart anatomy concept. 822 out of the 920 cases underwent angiocardiography, and 584 cases were confirmed by operation. RESULTS: According to three-segment heart anatomy concept of atrial position, 855 cases were situs solitus (including 89 cases of left superior vena cava and 31 cases of complete anomalous pulmonary venous drainage), 53 cases were situs inversus, and 12 cases were situs ambigus. As to atrial-ventricular valve, 747 cases were both opening, 112 cases had one-side atresia, and 61 cases had one together valve; As to ventricle position, 606 cases were dextro-loop, 111 cases had levo-loop, and 203 cases had single ventricle (including 24 cases), As to cor biloculare of great artery relation, 304 cases had normal relation of great arteries, including 35 cases of pulmonary atresia, 219 cases had right-transposition of great arteries, 240 cases had left-transposition of great arteries, 32 cases were mirror to normal, and 25 cases had persistent truncus arteriosus. 823 cases were diagnosed with 2DE, with a total accurate rate of 89.5%. CONCLUSION: Applying three-segment heart anatomy concept to 2DE to diagnose complicated CHD and analyzing the heart segment by segment will make the diagnosis simple and systematic, providing a concise basis for heart surgeries.
Adolescent↗
[Analysis of the methylation in CpG island by denaturing high-performance liquid chromatography].
OBJECTIVE: To establish a new approach for analyzing methylation circumventing the limitations of the existing methods. METHODS: A region containing CpG sites in mismatch repair gene hMLH1 promoter was amplified by strand-specific polymerase chain reaction (PCR) after sodium bisulfite treatment. The retention time of the PCR product at partially denaturing temperature was determined by denaturing high-performance liquid chromatography (DHPLC). The methylation status obtained by DHPLC was proved by comparing it with that from sodium bisulfite-restriction enzyme digestion. RESULTS: Methylation of hMLH1 promoter from a colorectal cancer cell line RKO and a gastric cancer cell line PACM82 was analyzed by DHPLC. The retention time of the PCR product from RKO was obviously longer than that from PACM82 (6.7 min vs. 6.2 min). Thus, it was concluded that the hMLH1 promoter from RKO was methylated, while that from PACM82 was not, since the longer retaining time of RKO was due to the higher C/G content after sodium bisulfite treatment. The results from DHPLC were consistent with those from sodium bisulfite-restriction enzyme digestion. CONCLUSION: DHPLC method is a rapid and reliable approach for analyzing methylation in CpG island of the sequence of interest.
Adaptor Proteins, Signal Transducing↗
Ovine metabolism of saponins: evaluation of a method for estimating the ovine uptake of steroidal saponins from Narthecium ossifragum.
A sheep was dosed three times per day over six consecutive days with 70 g Narthecium ossifragum, and once on the seventh day with 70 g N. ossifragum. Additionally, it was dosed once on days 1-7 with 20 mg of [20,23,23-2H3]sarsasapogenin. After 7 days, the sheep was killed and GC-MS analysis of the free and conjugated sapogenin content in bile, urine, rumen, duodenum, jejunum, colon and rectum samples collected from the sheep, faecal samples collected on days 4-7, and dosed plant material was performed. The N. ossifragum contained mainly sarsasapogenin and smilagenin. Only neglible levels of deuterium-labelled sarsasapogenins were detected in the samples from the animal. Ingested saponins were quickly hydrolysed in the rumen to free sapogenins and, in part, epimerized at C-3 to afford episapogenins. The absorption of free sapogenins appeared to occur in the jejunum. The concentration of sapogenins in faeces reached a plateau 108 h after dosing started.
Animals↗
[The effect of lip repair on maxillary growth in patients with complete unilateral cleft lip and(or) palate].
OBJECTIVE: To investigate the different effects of lip repair on maxillary growth among patients with cleft lip and(or) palate, and also the possible mechanisms accounting for that differences. METHODS: The lateral cephalometric analysis was applied to 84 Chinese patients with unilateral cleft lip and(or) palate at the early stage of permanent dentition, which included 32 UCLA, 20 UCLP (CP unrepaired), and 32 UCLP, and 37 normal Chinese of the same age as controls. RESULTS: The patients with unilateral cleft lip and palate who had lip repaired while the palate was left unrepaired exhibited the similar inhibition on maxillary growth to those who had both lip and palate repaired but the patients with unilateral cleft lip and alveolus had much less inhibition on their maxilla. CONCLUSIONS: The cleft and tissue defect of patients with cleft lip and palate might be the main cause that induced the inhibitive effects of lip repair on maxillary growth.
Cleft Lip↗
[Inhibitive effects of lip repair on maxillary growth in patients with complete unilateral cleft lip and palate].
OBJECTIVE: The aim of this study was to assess the isolated effects of lip repair on inhibition of maxillary growth in patients with complete unilateral cleft lip and palate. METHODS: The lateral cephalometric analysis were applied to 20 patients with unilateral cleft lip and palate who only had lip repaired in childhood, 32 patients with unilateral cleft lip and palate who had both lip and palate repaired in childhood, as well as 37 normal Chinese of the same age as controls. RESULTS: Both complete unilateral cleft lip and palate groups had almost the same significant degree of maxillary retrusion compared with the normal control group. CONCLUSION: Lip repair is one of important factors that could inhibit maxillary growth in patients with complete unilateral cleft lip and palate.
Adolescent↗
Formation of N-(Nitrosomethyl)urea in stomachs of experimental pigs and human volunteers given fish sauce in vivo.
N-(Nitrosomethyl)urea (NMU) was characterized in carcinogenic nitrosated fish sauce recently (Deng et al. J. Agric. Food Chem. 1998, 46, 202-205; Biomed. Environ. Sci. 1999, 12, 54-61). To study the possibility of intragastric synthesis of NMU, experimental mini-pigs surgically fitted with a flexible stomach cannula and human volunteers were used. Fish sauce samples (20-30 mL) and nitrite were injected into the gastric lumen through the cannula for pigs or taken orally for human volunteers. Gastric juice samples were taken out 30 min later. Concentration of NMU in condensed extracts of these samples was analyzed with HPLC-photohydrolysis-pyrolysis-thermal energy analyzer. Results showed that there was formation of NMU in the gastric lumen of both models in vivo and that the formation of NMU was nitrite- and pH-dependent. NMU was also detectable in the condensed extract of 100 mL of a mixture of pooled fasting human gastric juice samples and fish sauce sample (9:1, v/v) after treatment with 500 micromol/L of nitrite in vitro. In conclusion, there is intragastric formation of NMU, even at natural amounts of nitrite.
Adult↗
[Study on composition of plaque fluid in individuals with no oral hygiene].
OBJECTIVE: To study the change of composition in long-term plaque fluid after sucrose rinse and the cariogenicity of plaque. METHODS: 43 subjects, who had no regular oral hygiene and no tooth brushing at least for one year, were selected. According to their caries status, 27 as caries-free group and 16 as caries-positive group. Plaque were obtained before and at 7 minutes after a 10% sucrose rinse. Additionally, plaque samples of 15 and 30 minutes after sucrose rinse from 11 subjects were collected. The pH value and the concentrations of organic acids and inorganic ions in plaque fluid were measured. RESULTS: At 7 minutes after sucrose rinse, pH and the concentration of ammonium were significantly decreased, and the concentrations of lactic acid and formic acid were significantly increased. All these changes were similar to those of short-term plaque. No significant difference with any single component of plaque fluid was found between caries-free group and caries-positive group, neither before sucrose rinse nor after sucrose rinse. CONCLUSIONS: Changes of the composition in the plaque fluid were basically similar between long-term and short-term plaque, but single component in plaque fluid could not reflect the cariogenicity of plaque.
Adult↗
Diabetes-induced vascular dysfunction in the retina: role of endothelins.
AIMS/HYPOTHESIS: Vasoactive factors like endothelins, by virtue of the microvascular regulation as well as by other effects, possibly play important parts in the pathogenesis of diabetic retinal microangiopathy. We investigated retinal vascular dysfunction in streptozotocin-induced diabetes and its relation with endothelins in short- and long-term diabetes. METHODS: Diabetic rats with or without an endothelin receptor antagonist (bosentan) treatment were investigated after 1 month and 6 months of follow-up. Retinal blood flow was measured and compared with age- and sex-matched non-diabetic control animals. Retinal tissues were analysed for endothelin-1, endothelin-3, endothelin A and endothelin B mRNA. Distribution of endothelin-1 and endothelin-3 was investigated by immunocytochemistry and that for endothelin receptors by ligand binding and autoradiography. RESULTS: Diabetic animals showed hyperglycaemia, glycosuria, elevated glycated haemoglobin values and reduced body weight gain. Retinal blood flow showed an increased resistivity index, an indicator of vasoconstriction, after 1 month of diabetes which was prevented by treatment with bosentan. This functional change in diabetes was eliminated after 6 months of follow-up. The retina from the diabetic animals showed increased mRNA expression for endothelin-1, endothelin-3 and endothelin A after one month. In addition, endothelin B mRNA expression was increased after 6 months. Furthermore, endothelin-1 and endothelin-3 immunoreactivity and endothelin receptor concentrations were increased in the retina of diabetic rats. CONCLUSION/INTERPRETATION: The results from this study indicate that the endothelin system is of importance in mediating retinal changes in diabetes although mechanisms of the endothelin system alteration as well as their effects might vary depending on the duration of diabetes. [Diabetologia (1999) 42: 1228-1234]
Animals↗
Chromogranin A in the central nervous system of the rat: pan-neuronal expression of its mRNA and selective expression of the protein.
Chromogranin A, a glycoprotein stored in secretory granules of neuroendocrine cells, displays a widespread distribution throughout the central nervous system of a variety of species. In situ hybridization histochemistry was employed to investigate the localization of chromogranin A mRNA in the central nervous system of the rat. The previously characterized monoclonal antibody, LK2H-10, was employed in an immunohistochemical study to compare the topographic localization of the chromogranin A protein with that of its mRNA. Although the latter, as revealed by in situ hybridization, displayed a ubiquitous, pan-neuronal localization throughout the rat brain, LK2H-10 immunoreactive cell bodies and axon terminals were disposed in a widespread, but highly regionally differential, distribution. This discrepancy suggests that chromogranin A is processed in a regionally differential fashion in the rat brain to yield one or multiple variant forms, one of which is specifically recognized by LK2H-10. Catecholaminergic cell groups consistently displayed LK2H-10 immunoreactivity. LK2H-10 immunopositive axon terminals were prominent in the circumventricular organs. In addition, LK2H-10 immunoreactivity was also detected in a subset of astrocytes which demonstrated a widespread, but anatomically restricted, pattern of distribution. Consequently, the variant of chromogranin A labelled by LK2H-10 represents a novel neurochemical marker for regionally differential astrocytic diversity.
Animals↗
[Review on experimental studies of Chinese drugs in antagonizing bone marrow inhibition caused by chemicals].
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Animals↗
[A study on formation of N-(nitrosomethyl) urea in experimental pig stomach gavaged with fish sauce].
OBJECTIVE: To study formation of N-(nitrosomethyl) urea (NMU), which is categorized as N-nitrosamides -- a sort of strong chemical carcinogen, by perfusion with fish sauce via pig stomach fistula, which is used daily as cooking flavor by local residents in Changle County, Fujian Province, a highly-prevalent area for gastric cancer. METHODS: Fistulization was performed in fasting experimental pigs' stomach, and their gastric juice was suctioned 30 minutes after perfusion with fish sauce and sodium nitrite via fistula. Gastric juice specimen was purified and concentrated and determined for NMU with high performance liquid chromatography-photolysis pyrolysis-thermal energy analyzer. RESULTS: NMU was detected in the pig stomach at pH 1 - 2, where NMU formed. Formation of NMU depended on the amount of nitrite added in a dose-dependent relationship. Level of NMU in gastric juice reached 25.4 and 7.97 micromol/L, respectively, when 3.48 and 0.87 mmol of sodium nitrite were fed into the stomach. No NMU could be detected, as 0.22 mmol of sodium nitrite fed. CONCLUSION: Under the condition of pH 1 - 2 and presence of sodium nitrite, NMU was synthesized in the stomachs of experimental pigs fed with fish sauce via gastric fistula. It is postulated that N-nitrosamides, such as NMU could be formed endogenously in the stomachs of local residents who consume fish sauce often and have a higher exposure to nitrite in their stomachs.
Animals↗
Corneal topographical changes following strabismus surgery.
PURPOSE: To study corneal topographical changes after strabismus surgery. METHODS: Computer-aided corneal topography was used in 43 strabismus patients (45 eyes) one or two days prior to and six or seven days after strabismus surgery. The spherical and cylindrical equivalents were calculated based on the simulated keratometry. RESULTS: After the surgery, only the changes at 3 mm in the inferior quadrant were statistically significant. The changes at 3 mm in the rest quadrants and the changes at 7 mm were not significant. Significant changes in spherical equivalent were found post-operatively. Neither the horizontal nor the vertical meridional equivalent showed significant changes after surgery. CONCLUSIONS: The results of corneal topographical changes following strabismus surgery in our preliminary study indicated the little effect of strabismus surgery on corneal curvature and corneal astigmatism.
Adolescent↗
Characterization of N-(Nitrosomethyl)urea in Nitrosated Fermented Fish Products.
To characterize chemical carcinogens in acidic-nitrosated fish sauce sample, N-nitrosamides in the sample were separated by two kinds of reversed-phase HPLC columns, and with detection by photolysis-pyrolysis-thermal energy analyzer. A strong chromatographic peak at t(R) 12 or 4.5 min, same as that for N-(nitrosomethyl)urea (NMU), was obtained on PRP-1 or C(18) HPLC column from fish sauce sample with 10 mM trifluoroacetic acid as the basic mobile; acetonitrile, as organic modifier after the sample was nitrosated by 5 mmol/L of sodium nitrite (final concentration) at 37 degrees C and pH 2.0 for 1 h. No response above t(R) could be observed from the nitrosated sample in the detection system without photolysis. Such a peak could not be obtained from the unnitrosated fish sauce either. These results indicated that the component was NMU. Furthermore, this component, NMU, could also be detected in the nitrosated human gastric juice sample spiked with fish sauce. The formation of NMU in the sample was pH- and nitrite-dependent. This paper provides direct evidence that NMU formation could occur in fish sauce from the high-risk area for stomach cancer and in the fish sauce spiked human gastric juice during nitrosation under simulated gastric conditions.
Journal Article↗
Parathyroid hormone-related proteins is abundant in osteoarthritic cartilage, and the parathyroid hormone-related protein 1-173 isoform is selectively induced by transforming growth factor beta in articular chondrocytes and suppresses generation of extracellular inorganic pyrophosphate.
OBJECTIVE: Parathyroid hormone-related protein (PTHrP) is a major, locally expressed regulator of growth cartilage chondrocyte proliferation, differentiation, synthetic function, and mineralization. Because mechanisms that limit cartilage chondrocytes from maturing and mineralizing are diminished in osteoarthritis (OA), we studied PTHrP expression by articular chondrocytes. METHODS: PTHrP was studied in normal knee cartilage samples and cultured articular chondrocytes, and in cartilage specimens from knees with advanced OA, obtained at the time of joint replacement. RESULTS: PTHrP was more abundant in OA than in normal human knee articular cartilage. Both demonstrated PTH/PTHrP receptor expression. PTHrP 1-173, one of three alternatively spliced PTHrP isoforms, was exclusively expressed and induced by transforming growth factor beta in cultured chondrocytes. Chondrocytes mainly used the GC-rich P2 alternative promoter to express PTHrP messenger RNA. Inhibition by PTHrP 1-173, but not by PTHrP 1-146 or PTHrP 1-87, of inorganic pyrophosphate (PPi) elaboration suggested selective functional properties of the 1-173 isoform. Exposure to a neutralizing antibody to PTHrP increased PPi elaboration by articular chondrocytes. CONCLUSION: Increased expression of PTHrP, including the 1-173 isoform, has the potential to contribute to the pathologic differentiated functions of chondrocytes, including mineralization, in OA.
Adult↗
[The influence of implantal autograft bone in total cleft palate on maxillary growth an experiment study in dogs].
OBJECTIVE: To test the hypothesis that implant autograft bone in total cleft palate at primary cleft palate repair which may resists the disturbance of maxillary growth by cleft and denud bony wound. METHODS: 40 dogs were randomly divided into four groups: three control groups and one experimental group. The first control group was unoperated, the second group had a surgically created cleft plate, the third group also had a surgically created cleft palate. Using a two-flap procedure immidiately, the experiment group had a surgically created cleft palate, but the palate was recontracted by implanting a piece of autograft rib bone with two-flap repair. All animals were sacrificed on the 34th week after operation (43 weeks postnatal). Thirty-four metricranial variables were measured directy from the cleaned skulls. RESULTS: The method is useful for maxillary growth and development by bone implant in total cleft palate, but it can cause maxillary deformity. CONCLUSION: Clinical doctors should be cautious in using the method of primary-delay bone grafting in reparing total cleft of cleft palate.
Animals↗