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Biomedical subjects

D J Blazevic

Publications and source records attributed to D J Blazevic.

At least 37 records · Page 2Linked to original sources
PubMed1976

Significance of urinary isolates of coagulase-negative Micrococcaceae.

Of 16,347 urine cultures submitted to the hospital laboratory, 68 (0.4%) specimens from 50 patients yielded greater than 10(4) coagulase-negative staphylococci/ml in pure culture. A total of 62 of 63 organisms available for study were staphylococci: 45 Staphylococcus epidermidis (predominantly subgroup 1), 15 Staphylococcus saprophyticus (subgroup 3), and 2 Staphylococcus aureus. Twenty-one patients had "probable" urine infections. Eight patients had two or more positive urine cultures, and all isolates from the same patients were identical (by morphology, antibiotic susceptibility, and hemolytic pattern). Nine (75%) of the 12 isolates of S. saprophyticus, which were novobiocin resistant and nonhemolytic on the synergistic hemolysis test, were from patients with probable urinary infection. Eight were young women with acute symptoms and pyuria. Differences in the glucose and mannitol fermentation tests with different media may lead to difficulties in identification. Novobiocin resistance cannot be relied upon to differentiate isolates of S. saprophyticus from S. epidermidis.

Adolescent↗
PubMed1976

Inoculation of API-20E from positive blood cultures.

The API-20E system (Analytab Products, Inc., Plainview, N. Y.) was inoculated from 4- to 6-h tryptic soy broth cultures that had been inoculated from positive blood cultures containing gram-negative bacilli. This method gave the same genus and species identification for 139 of 140 organisms (47 patient and 96 simulated positive cultures) when compared to the Analytab Products, Inc., recommended method of inoculation.

Bacterial Infections↗
PubMed1975

Evaluation of the modified broth-disk method for determining antibiotic susceptibilities of anaerobic bacteria.

A total of 110 strains of anaerobic bacteria including 9 different genera was tested for antibiotic susceptibility by the modified broth-disk method of Wilkins and Thiel (1973), using prereduced media and the VPI anaerobic culture system. Results were compared with those obtained with an agar dilution technique with incubation in a GasPak jar. There was greater than 95% agreement between the two methods with ampicillin, cephalothin, chloramphenicol, and clindamycin, and 90% or greater agreement with erythromycin, penicillin, and tetracycline.

Anaerobiosis↗
PubMed1975

Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures.

Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the aerobic bottle only, and 20% from the anaerobic bottle only. These results indicate the need for use of both an aerobic and anaerobic bottle for blood cultures.

Aerobiosis↗
PubMed1975

Motility-indole-lysine-sulfide medium.

A medium designed for the detection of motility, indole, lysine decarboxylase and deaminase reactions, and H2S production was devised and evaluated. Results, using 157 strains of enteric pathogens, were in agreement with reference methods. When 300 isolates from fecal cultures were screened using this medium, Shigella was easily differentiated from Escherichia and more of the Proteus species, especially P. morganii, could be eliminated from further study.

Agar↗
PubMed1974

Susceptibility of anaerobic bacteria to carbenicillin.

One hundred and seventy-one strains of anaerobes were tested for susceptibility to carbenicillin by using agar dilution, broth dilution, and two disk diffusion methods. The minimal inhibitory concentration (MIC) for 67% of 51 strains of Bacteroides fragilis, 7 of 9 strains of Bacteroides melaninogenicus, and all of 8 strains of Eubacterium was 100 mug or less per ml. The MICs of the remaining anaerobes were 50 mug or less per ml. The broth dilution results were felt to be the most accurate of the four methods utilized.

Anaerobiosis↗
PubMed1974

Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.

Blood was cultured in two vaccum bottles containing Columbia broth with sodium polyanethol sulfonate and CO(2). Filtered air was admitted to one bottle, and the bottles were incubated at 35 C until growth was detected or for a maximum of 7 days. Bottles were examined daily for macroscopic growth. Gram stains were made routinely on the 1st, 4th, and 7th days, and samples were routinely subcultured to sheep blood agar (incubated in GasPak jar) and chocolate agar (incubated in CO(2)) on the 1st and 4th days of incubation. Of 1,127 positive blood cultures, 65% were first detected by macroscopic examination, 23% were first detected by Gram stain, and 12% were first detected only by subculture.

Anticoagulants↗
PubMed1974

Biochemical and antibiotic susceptibility studies of H2S-negative Citrobacter.

Ninety-four strains of H(2)S-negative Citrobacter were biochemically characterized and their antibiograms were determined. The antibiograms demonstrated not only a difference from Enterobacter cloacae but also a difference within the Citrobacter group between the indole-negative and indole-positive strains. These differences were statistically significant and emphasize the importance of the indole reaction as an aid to speciation of the H(2)S-negative Citrobacter.

Anti-Bacterial Agents↗
PubMed1974

Rapid methods for biochemical testing of anaerobic bacteria.

Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-beta-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for esculin and gelatin showed an agreement of 86 and 77%, respectively. PathoTec test strips for nitrate, indole, esculin, o-nitrophenyl-beta-D-galactopyranoside, Voges-Proskauer, and urease were also tested and showed encouraging results.

Anaerobiosis↗
PubMed1973

Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae.

A rapid bioassay for gentamicin levels in serum was developed by using a strain of Klebsiella pneumoniae that has multiple resistance to antibiotics. Assays were comparable when performed on either small or large petri plates, and results were available in 2 to 4 h. Studies showed an overall recovery of 97.6% for gentamicin alone or 104.5% in the presence of commonly used antibiotics. The procedure can be performed without the need to inactivate other antibiotics that may be present in the serum sample.

Biological Assay↗
PubMed1973

Incidence and identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory.

Strains of Pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. A rapid nitrate test was found to be useful in distinguishing P. aeruginosa (positive) from P. fluorescens and P. putida (both negative). A shortened gelatin test differentiated P. fluorescens (positive) from P. putida (negative). P. fluorescens and P. putida were very sensitive to low levels of kanamycin and resistant to carbenicillin, a pattern just the opposite of that obtained with P. aeruginosa.

Anti-Bacterial Agents↗
PubMed1973

Evaluation of the PathoTec "Rapid I-D System".

The 10 biochemical test strips included in the PathoTec Rapid I-D System were evaluated for accuracy as compared to standard tests and for efficacy in identification of 193 gram-negative bacilli. The test agreement was 100% for oxidase and phenylalanine deaminase, 99% for indole, nitrate, and Voges-Proskauer, 98% for malonate, 97% for lysine decarboxylase, 90% for urease, 84% for H(2)S, and 75% for esculin hydrolysis. Most of the commonly isolated Enterobacteriaceae were identified correctly within 4 h. Errors in identification of Proteus morganii and P. rettgeri occurred because of positive H(2)S tests on the PathoTec strips with these organisms.

Bacteriological Techniques↗
PubMed1972

Nucleic acid enzyme studies of nonfermentative gram-negative bacteria using thin-layer chromatography.

A rapid and easy to perform procedure for determining the nucleic acid enzyme reactions of intact bacterial cells was developed. Overnight organism growth on triple sugar agar was tested for nucleoside phosphotransferase, nucleosidase, and nucleotidase activity. Reaction products were detected by means of thin-layer chromatography and fluorescence. Characteristic patterns were seen with certain strains of nonfermentative gram-negative bacteria, which indicated that these tests could aid in classification and identification of E0-1 group, Pseudomonas multivorans, P. cepacia, P. maltophilia, certain other Pseudomonas species, and Herellea vaginicola.

Acinetobacter↗