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Biomedical subjects

D J Blazevic

Publications and source records attributed to D J Blazevic.

51 records · Page 3Linked to original sources
PubMed1972

Characterization of indole-positive Proteus mirabilis.

Thirteen indole-producing, swarming strains of Proteus were identified by additional biochemical testing as being Proteus mirabilis. These strains were characterized by 40 biochemical tests and by susceptibility testing to 11 antibiotics. All produced ornithine decarboxylase and were susceptible to members of the penicillin-cephalosporin groups of antibiotics. These indole-positive strains are similar to indole-negative P. mirabilis and are distinctly different from P. vulgaris. For greatest accuracy and to insure greatest clinical relevancy, P. mirabilis and P. vulgaris should be distinguished from one another in the laboratory by performing both the indole and ornithine decarboxylase tests.

Ampicillin↗
PubMed1971

Antibiotic susceptibilities of Serratia marcescens and Enterobacter liquefaciens.

Production of 5'-nucleotides by Serratia marcescens and Enterobacter liquefaciens correlates with deoxyribonuclease production, indicating the close relationship between these two organisms. To determine further relationships, susceptibilities of 279 strains of the tribe Klebsielleae were determined by the high-potency disc method, agar-dilution method, or both, by using 14 antibiotics. Ninety-seven per cent of S. marcescens (201 of 207 strains) and 100% of E. liquefaciens (17 strains) had minimum inhibitory concentration (MIC) of 100 mug/ml or greater with colistin and polymyxin B. With these two antibiotics, 93% of other Enterobacter species (28 strains) had MIC values of less than 1.6 mug/ml, and 100% of Klebsiella (27 strains) had MIC values less than 1.6 mug/ml. Consistent patterns were not noted with the other antibiotics tested, but the results with colistin and polymyxin B provide additional evidence of the close relationship of S. marcescens and E. liquefaciens.

Anti-Bacterial Agents↗
PubMed1971

Detection of arginine dihydrolase in nonfermentative gram-negative bacteria by use of thin-layer chromatography.

Thin-layer chromatography was used to detect the presence of the arginine dihydrolase system in nonfermentative gram-negative bacteria. The test was positive for the fluorescent pseudomonads as well as Pseudomonas maltophilia, whereas other Pseudomonas sp., Mima, and Herellea were negative. This procedure can be completed in a few hours and may be useful in the clinical laboratory.

Acinetobacter↗
PubMed1970

Differentiation of Serratia from Enterobacter on the basis of nucleoside phosphotransferase production.

Precoated cellulose thin-layer chromatograms were used to detect the production of guanosine 5'-phosphate from guanosine and p-nitrophenylphosphate by whole-cell preparations. One-hundred per cent of Serratia (163 strains) and 84% of E. liquefaciens (15 of 18 strains) produced the nucleotide. All other Enterobacter (23 strains), Klebsiella (10 strains), and E. coli (10 strains) were negative for the production of this nucleotide. The entire test procedure could be carried out in 4 hr. It is proposed that E. liquefaciens is more closely related to Serratia than Enterobacter and that reclassification of these organisms should be investigated.

Chromatography, Thin Layer↗
PubMed1970

Evaluation of a new incubation-type indole strip test.

A total of 189 of Enterobacteriaceae and three strains of Clostridium perfringens were tested with a new incubation-type indole paper strip. This 4-hr incubation test gave the same results as a standard 48-hr indole test, except with some strains of Proteus rettgeri. Results with P. rettgeri varied somewhat according to the medium upon which the organisms had been grown.

Bacteriological Techniques↗
PubMed1969

Selection of media for the isolation of common bacterial L-phase organisms from a clinical specimen.

The L-phase of 13 bacteria commonly associated with disease were induced by penicillin and inoculated into various solid and broth media; their growth was recorded for a period of 14 days. Plates containing highly purified agar and sucrose as the stabilizing agent and those incubated under aerobic conditions gave the best results. Magnesium seems to be necessary for growth in broth media on primary isolation, although it may not be necessary on multiple transfers after a more stable state has been reached. Growth in broth media is much more difficult to achieve. Reversion is aided by using a higher concentration of agar in plates, by decreasing the sucrose concentration, and by omitting the antibiotics and horse serum. A procedure has been outlined for the routine culture and identification of L-phase organisms from a clinical specimen.

Bacteria↗
PubMed1969

Characterization of ornithine decarboxylase-positive, nonmotile strains of the Klebsiella-Enterobacter group.

Thirty-seven strains of ornithine decarboxylase-positive, nonmotile Klebsiella-Enterobacter organisms isolated from 36 patients were studied by biochemical and serological testing. Five strains gave biochemical reactions which conformed closely to those of Escherichia coli; three strains gave positive Quellung reactions to specific Klebsiella antisera. (Two of these were thought to be Enterobacter in spite of this typing reaction.) The remaining 29 strains were classified as Enterobacter. These results demonstrate the necessity of doing both an ornithine decarboxylase test and a motility test to differentiate Klebsiella from Enterobacter. Had only a motility test been done, they all would have been called Klebsiella.

Carboxy-Lyases↗