Biomedical subjects

D M Pettit

Publications and source records attributed to D M Pettit.

4 recordsLinked to original sources

PubMed2002

Attempts to immunize sheep against the scab mite, Psoroptes ovis.

Adult Psoroptes ovis were successively extracted in saline, 1% Tween, 1% Triton and 8 m urea +0.1% CHAPS. The Triton extract was separated into fractions which did or did not bind to ConA lectin. Using QuilA as adjuvant, both Triton fractions and the saline, Tween and urea extracts were tested separately as candidate protective antigens against a P. ovis challenge infestation in sheep. All induced circulating antibody responses, but the saline and Tween extracts also stimulated significant protective effects in those sheep which developed active disease. Compared to control sheep injected with adjuvant only, these immunized animals had more than two and seven-fold reductions in mean lesion areas and mite numbers, respectively.

Animals↗

PubMed1995

Discharge readiness assessment.

Discharge planning has a long and varied history in health care delivery. As length of stay declines, the health care provider has less time to educate patients and families about home care and less time to coordinate services from home health and community agencies. As a result, many patients and families are discharged with unmet home care needs and thus are at an increased risk for complications and hospital readmissions. Readiness for discharge is one part of discharge planning and is a multifaceted, multistage concept that provides an estimate of patients' and their family members' ability to leave an acute care facility. Discharge readiness assessment is the evaluation of strengths and needs in five areas: physiologic stability, competency (cognitive and psychomotor) of the patient and family to carry out self-care management regimens, perceived self-efficacy to carry out self-care management regimens, availability of social support, and access to the health care system and community resources. Each of these areas is presented as a component of discharge readiness assessment.

Chronic Disease↗

PubMed1995

The use of gauze versus transparent dressings for peripheral intravenous catheter sites.

The purpose of this research utilization project was to determine if a sufficient research base was present to support the use of gauze rather than transparent dressings for peripheral intravenous catheter sites. The research articles reviewed in this article do not support a relationship between dressing type and complications of peripheral intravenous catheters.

Adult↗

PubMedDate not supplied

Immunization against sheep scab: preliminary identification of fractions of Psoroptes ovis which confer protective effects.

In an attempt to enrich for potentially protective Psoroptes ovis antigens, three separate vaccine trials were conducted in which groups of sheep were immunized three times with various fractions of a soluble extract of P. ovis mites using QuilA as adjuvant. These groups, as well as controls that received adjuvant only, were challenged with P. ovis, and protective immunity was assessed by measuring lesion areas and conducting mite counts 4 and 6 weeks later. All fractions stimulated high titre serum antibodies. Most conferred some protection on sheep with active disease, although there was considerable variation between sheep in all groups, including the controls. Some fractions were more protective than the extract itself, suggesting that the protective components had been concentrated. Indeed the best fraction, obtained by ion exchange chromatography, followed by a gel filtration step, slowed lesion growth to less than a third by 6 weeks after challenge and reduced mite numbers by more than 13 times compared to control sheep vaccinated with QuilA only. However, as judged by polyacrylamide gels, the polypeptide profile of this fraction was still complex, indicating that further work is required to identify the protective components.

Adjuvants, Immunologic↗