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Biomedical subjects

D Moore

Publications and source records attributed to D Moore.

At least 289 records · Page 16Linked to original sources
PubMed1986

Hepatitis A virus replication in tamarins and host immune response in relation to pathogenesis of liver cell damage.

Hepatitis A virus (HAV) shedding in the faeces, appearance of HAV-Ag (antigen) in the liver, and development of humoral immunity to HAV have been studied in experimentally infected tamarins. The appearance of liver damage measured by transaminase elevation and histology, in relation to the above variables, suggests that the virus is not cytopathic and the immune system contributes to the production of liver cell damage. Preliminary data suggest that HAV replication may occur in the mucosa of the small intestine and in the liver.

Animals↗
PubMed1986

Effects on the rabbit carotid body of stimulation by almitrine, natural high altitude, and experimental normobaric hypoxia.

Rabbits were given intraperitoneal injections of almitrine in ascending doses for 5 weeks. They were compared with a control group and with a group of rabbits which had been exposed from birth to the natural hypobaric hypoxia found at Cerro de Pasco (433 m) in the Peruvian Andes. A further group of animals was placed in an experimental normobaric chamber for either 3 or 6 months to subject them to the same degree of hypoxia as that occurring in Cerro. The carotid bodies of the rabbits in all these groups were processed for light and electron microscopy, and examined both qualitatively and quantitatively. The carotid bodies in the group given almitrine showed no changes in their size or in the population of their glomic cells when compared with controls. In contrast, the carotid bodies of Peruvian rabbits were greatly enlarged with a disproportionate increase in the population of the light variant of chief cell. Rabbits from the hypoxic chamber also had enlarged carotid bodies but those killed after 3 months showed an increase in the dark variant of chief cell, whereas after 6 months this cell was reduced in number. There was also intense cytoplasmic vacuolation. Election microscopy confirmed these changes and revealed that dark cells had larger, more pleomorphic granules than the light variant. Vacuolation of the granules in light cells was most pronounced in Peruvian rabbits, but was uncommon in animals exposed to hypoxia for 3 months. We suggest that the dark cell responds to the early stages of hypoxia but later matures into the light variant of chief cell.

Almitrine↗
PubMed1986

Chronic intussusception due to antral myoepithelioma.

Intussusception was seen on abdominal sonography and computed tomography in a 15-year-old boy who presented with a 6-week history of weight loss, vomiting, abdominal pain, abdominal mass, and hyperamylasemia. Laparotomy revealed a chronic gastroduodenal intussusception, the lead point of which was an antral myoepithelioma, a rare entity in this age group.

Adolescent↗
PubMed1986

Operative recovery of vasal sperm from anejaculatory men: preliminary report.

Sperm showing good motility and function, as demonstrated by the in vitro sperm penetration assay using hamster ova, were collected from the vas deferens of 3 men with ejaculatory failure. The collection procedure may be repeated over multiple ovulatory cycles. The sperm may be used to attempt artificial insemination or in vitro fertilization.

Adult↗
PubMed1986

Eosinophilic gastroenteritis presenting in an adolescent with isolated colonic involvement.

Eosinophilic gastroenteritis most commonly involves the stomach and proximal small intestine with eosinophilic inflammation of either the mucosa, submucosa or serosa. The patient reported here had isolated eosinophilic colitis. The initial presentation with iron deficiency anaemia owing to occult gastrointestinal blood loss emphasises the need to evaluate the entire gastrointestinal tract in patients with eosinophilic gastroenteritis.

Adolescent↗
PubMed1985

Calcium-activated cation channel in rat thyroid follicular cells.

Using the patch-clamp single-channel current recording technique, a cation channel in the contraluminal membrane of rat thyroid follicular cells has been characterized. The channel has a unit conductance of about 35 pS and is equally permeable to sodium and potassium. The pattern of channel opening and closing is independent of the membrane potential. The channel is only operational when the ionized calcium concentration in the fluid which is in contact with the inside of the membrane is at least 1 microM. This conductance pathway can be classified as a calcium dependent non-selective cation channel and could explain stimulant-evoked depolarizations in the thyroid follicular cells.

Animals↗
PubMed1985

Aboriginal pregnancies and births in South Australia, 1981-1982.

Information on 555 Aboriginal births which occurred during 1981 and 1982 was forwarded by midwives to the South Australian perinatal statistics unit. Corresponding information was also supplied for all other births in the state. This information showed that Aboriginal women appear to have a higher fertility rate than do other women, particularly in the teenage years. Aboriginal mothers are very young and have a high parity. They appear to receive little antenatal care and there is a greater tendency for their pregnancies to be complicated by medical conditions, such as anaemia, urinary tract infections, cardiac disorders and diabetes. Post-partum haemorrhages and retained placentas are relatively common, as are genital tract infections after delivery. Aboriginal babies are characterized by low birthweights, low Apgar scores, and prematurity. There is an indication that Aboriginal babies may have a high perinatal mortality rate in the country areas of South Australia. It is intended that this information be used as a baseline for evaluating trends in the health status of Aborigines.

Adolescent↗
PubMed1985

Genes and gene products involved in the synthesis of F-pili.

Membrane fractions containing [35-S]methionine labeled proteins synthesized by Flac and Flac tra mutant strains or by lambda tra transducing phages expressed in such strains have been analyzed in order to investigate the pathway for synthesis of the F-pilin subunit and the gene products involved in synthesis of F-pili. Our data indicate that the synthesis of a mature F-pilin subunit requires the expression of at least 2 tra operon genes in addition to the structural gene for F-pilin, traA. In the absence of these activities, traA expression results primarily in the synthesis of a polypeptide, Ap14, with an apparent molecular weight of approximately 14,000. We assume this polypeptide corresponds to the direct product of the traA gene. In the presence of traQ activity, the major detectable product of traA is a polypeptide, Ap7(Q), which migrates with an apparent molecular weight of 7,000, suggesting that traQ product may process or assist in the processing of Ap14. Polypeptide Ap7(Q) is not, however, mature F-pilin, since it reacts poorly with anti-F-pilus-serum. Synthesis of a polypeptide which appears to be antigenically equivalent to F-pilin and which we assume requires a modification of the F-pilin N-terminus, is detected as synthesis of a polypeptide, Ap7*. This protein migrates slightly more slowly than Ap7(Q) on our polyacrylamide gels. Polypeptide Ap7*, can be efficiently precipitated with F-pilus antiserum, and can be detected in both inner membrane and outer membrane fractions under conditions where assembly of F-pili can occur. These data suggest that Ap7* is the mature F-pilin subunit and is assembled from an inner membrane pool. Synthesis of Ap7* appears to require traG activity, but may also be dependent upon additional tra activities.

Bacterial Outer Membrane Proteins↗
PubMed1985

Chromosomes of human sperm: variability among normal individuals.

The chromosomal constitution of 2468 human sperm cells has been investigated by fusion of human sperm with hamster eggs. The overall frequency of cells with structural aberrations was 7.7%, ranging from 1.9% to 15.8%, and varying significantly among individuals. The highest frequency occurred in sperm from the oldest donor (49 years), who also had had a vasectomy reversal three years prior to sampling. The overall aneuploidy frequency was 1.7%, ranging from 0.6% to 3.1%. In nine out of ten donors from whom blood samples were available the frequency of sperm cells with structural aberrations was higher than that for lymphocytes. Two previously reported donors (Brandriff et al. 1984) were resampled after an interval of 14 and 16 months respectively, and were each found to have similar frequencies of sperm chromosome abnormalities at both sampling times. A father-son pair included in the study had several chromosome breakpoints in common, although no more frequently than unrelated individuals.

Adult↗