PubMed Health⌕ Search

Biomedical subjects

D Moore

Publications and source records attributed to D Moore.

At least 307 records · Page 17Linked to original sources
PubMed1985

Incidence of cancer in surgically treated thyroid nodules based on method of selection.

A retrospective review of thyroidectomies to rule out cancer at Columbia Presbyterian Medical Center indicated that the maximum cancer incidence achievable in surgical specimens was only 57% if all microfollicular adenomas and thyroid cancers were removed. The size distribution of follicular cancer and microfollicular adenoma was similar and it does not appear to be useful in deciding which microfollicular lesions need to be surgically excised to exclude carcinoma. The incidence of thyroid cancer in patients undergoing thyroidectomy was not influenced by the introduction of fine-needle biopsy (FNB) at Columbia Presbyterian Medical Center. FNB did appear to increase the incidence of cancer at an affiliated community hospital. Coarse-needle biopsy definitely improved the incidence of cancer in patients undergoing thyroidectomy and was superior to FNB and clinical selection alone. A combination of FNB and coarse-needle biopsy when possible yielded a 41% incidence of cancer and appears to be the most useful technique for patient selection.

Adenocarcinoma↗
PubMed1984

Hyaluronate binding proteins also bind to fibronectin, laminin and collagen.

Small molecular weight proteins, isolated from the culture medium of embryonic chick heart fibroblasts and 3T3 cell lines by hyaluronate affinity chromatography, bind in order of apparent affinity, to hyaluronate, fibronectin, collagen and laminin. Such proteins isolated from the MSV-transformed 3T3 cell line bind in greater amounts to the nectins and hyaluronate than do similar proteins isolated from heart fibroblasts or 3T3 cells. These small hyaluronate binding proteins are immunologically distinct from other well characterized proteins such as laminin, fibronectin, bovine serum albumin and actin. Their relationship to other small, extracellular proteins and their possible role in structuring of extracellular matrix are discussed.

Animals↗
PubMed1984

Abdominal positioning interneurons in crayfish: projections to and synaptic activation by higher CNS centers.

Intracellular recording, stimulation, and Lucifer dye injections were used to characterize abdominal positioning interneurons from the neuropile of the second through sixth abdominal ganglia of the crayfish, Procambarus clarkii. Motor outputs of these cells were recorded with extracellular electrodes placed on various flexion and extension roots along the nerve cord. In an effort to assess the functional relationships between the postural interneurons in the abdomen and those known to exist in the circumesophageal connectives ( CECs ), a stimulus pulse train was delivered to each of the CECs while monitoring the intracellular responses of the impaled interneurons. Abdominal positioning interneurons were grouped into four general categories based on their responses to CEC stimulation: 1) those that projected their axons directly through the CECs ; 2) those that were remotely activated to spiking; 3) those locally activated to produce EPSPs or IPSPs; and 4) those that were not affected by CEC stimulation. The majority of abdominal positioning interneurons encountered in this study evoked flexion (N = 82), with relatively fewer evoking extension (N = 29). A major difference appeared between the two classes. Whereas 39% of the flexion interneurons had axons coursing to the brain, only 7% of the axons of extension interneurons coursed rostrally beyond the thoracic level. Finally, the large majority of those flexion and extension interneurons that lacked processes in the CEC received synaptic inputs at various levels along the lower CNS from other CEC neurons. Thus, control of abdominal positioning involves neurons at all levels in the CNS - some sequentially organized, others forming "through" pathways, but all with multiple input sites.

Action Potentials↗
PubMed1984

Metastasizing basal cell carcinoma developing in a gunshot wound in a black man.

Both basal cell carcinomas that arise in blacks and those that metastasize are rare, and development of this tumor is an uncommon, late complication of trauma. We report a case of a 70-year-old black man with a basal cell carcinoma arising in a gunshot wound in a shoulder and metastasizing to axillary lymph nodes. Histologically, the tumor was of the metatypical type.

Aged↗
PubMed1984

Synthesis of F-pilin polypeptide in the absence of F traJ product.

The products of a lambda transducing phage (ED lambda 101) which carries a segment of the F tra operon expressing F traA , traL , and traE activity from the lambda leftward promoter were examined using a uv-irradiated host system. After infection of an F- host, products of traE (19,500 Da) and traA (14,000 Da) were detectable among the lambda early proteins synthesized. Infection of an Flac host altered the pattern of polypeptides synthesized by the phage in that the 14,000-Da traA product became barely detectable and was replaced by a polypeptide which migrated at 7000 Da. A derivative of ED lambda 101 carrying the traA1 amber mutation was unable to synthesize either the 14,000-Da polypeptide in F- cells or the 7000-Da polypeptide in Flac cells. The 7000-Da polypeptide derived from ED lambda 101 was synthesized in the absence of traJ product in F- cells coinfected with a second transducing phage which carried a tra operon segment including traQ . It was also a product of ED lambda 134 which expresses genes traA through traH . The 7000-Da polypeptide, like F-pilin, associated primarily with the inner membrane, and could be immunoprecipitated with antiserum prepared against purified F-pili. Analysis of membranes from F- cells infected with ED lambda 101 indicated that the 14,000-Da traA product synthesized under these conditions accumulated in the inner membrane. These results show that both the 14,000-Da traA product might be processed to F-pilin in a traQ -dependent reaction which occurs in or on the inner membrane of the Escherichia coli host. However, the possibility that traQ encodes a regulatory product which affects expression of the traA sequence has not been excluded.

Bacterial Proteins↗
PubMed1984

Paraquat ingestion.

Explore the source record for details and available documents.

Acute Kidney Injury↗
PubMed1984

Tales from the 'Flying Doctor'.

Each year the Royal Flying Doctor Service provides medical attention to approximately 100,000 people in the more remote areas of Australia, including the aerial evacuation of about 9000.

Adult↗
PubMed1983

Cytomegalovirus-specific cell-mediated immunity during pregnancy in lower socioeconomic class adolescents.

The role of maternal cytomegalovirus (CMV)-specific cellular immunity in gestational CMV excretion and transmission of congenital infection was assessed by studying CMV-specific mononuclear proliferation (CMV-MP) longitudinally during pregnancy in 59 lower socioeconomic class adolescents. CMV-MP was absent in mothers seropositive for CMV on more than 40% of occasions tested, but no significant difference was noted at any time in frequency or degree of CMP-MP between the 16 mothers who shed virus and the 29 who were seropositive for CMV but did not shed virus. The presence of CMV-MP did not preclude gestational CMV excretion. The lone mother who bore a congenitally infected infant lacked CMV-MP on all three occasions when she shed virus. CMV-MP was noted in cord blood from 14 uninfected infants of seropositive mothers. Maternal CMV-specific cellular immunity is not the major determinant of gestational CMV excretion, but the extent of its role in modulating transmission of congenital infection is yet unclear.

Adolescent↗
PubMed1983

Physiology of F-pilin synthesis and utilization.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to study the synthesis and turnover of F-pilin in membrane preparations of Escherichia coli K-12 under conditions which have been reported to affect the production of F-pili. Incorporation of [(35)S]methionine into membrane F-pilin by cells in log phase was barely detectable at 25 degrees C, but increased with temperature. The labeled pilin band was prominent in membranes from 37 degrees C cultures and even more prominent if the growth temperature was raised to 42 degrees C. The appearance of other tra products in the membranes was similarly temperature dependent. In cultures grown in glucose minimal medium at 37 degrees C, the relative amount of membrane pilin and traT product synthesis remained unchanged from early log phase through early stationary phase; provision of glycerol or arabinose as a substitute carbon source had no obvious effect. Turnover of traT product and membrane F-pilin, as assessed in an Flac tra mutant strain which is incapable of elaborating pili, was not rapid. Both traT product and pilin subunits labeled in mid-log phase cells were still apparent in the membranes after growth of the cells to stationary phase. The relative amount of labeled pilin decreased with prolonged incubation in stationary phase, but the relative amount of traT product did not decrease even after the culture was incubated for 24 h. When wild-type Flac piliated cells were used, a similar result was obtained, but in this case, loss of F-pilin from the preparations could be acclerated by blending the cells. Although intermittent blending during culture growth caused a slow depletion of the labeled pilin pool, continuous blending resulted in the rapid disappearance of this pool from our preparations. Loss of other membrane polypeptides was not accelerated by our blending procedure, and blending did not affect the turnover of the pilin pool of the Flac tra mutant. Our data are consistent with a model in which pilin subunits are assembled transiently into pili, conserved by retraction, and made available for subsequent reassembly. Growth in 0.01% sodium dodecyl sulfate did not accelerate loss of pilin from the Flac strain compared with the Flac tra strain, and we suggest that in the presence of sodium dodecyl sulfate at this concentration, F-pili are not elaborated from cell surfaces.

Arabinose↗
PubMed1983

Cytomegalovirus-specific cell-mediated immunity in lower-socioeconomic-class adolescent women with local cytomegalovirus infections.

The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant adolescent women who also had CMV complement-fixing antibody titers and viral cultures of cervix, urine, saliva, and blood performed. Eighteen were studied more than once. No blood cultures were positive and no seroconversions were noted. There was no significant difference for frequency or degree of systemic CMV-specific blastogenesis between the 20 who were culture positive and the 41 who were seropositive but culture negative, although 40% of the culture-positive group and 27% of the seropositive, culture-negative group lacked CMV-specific blastogenesis. One of 31 seronegative subjects displayed CMV-specific blastogenesis. No systematic deficits were noted in any groups or individuals for E rosette number or mitogen response, though some isolated significant differences among groups for mitogen responses existed. Local CMV excretion in the study population was not related to systemic CMV-specific mononuclear blastogenesis.

Adolescent↗
PubMed1982

A new activity in the Ftra operon which is required for F-pilin synthesis.

Membrane preparations from a series of Hfr mutant strains of Escherichia coli K12 deleted in the promoter distal end of the F transfer operon were analyzed. Deletions which extended into traG, as expected, had no discernible effect on synthesis of membrane F-pilin. A more extensive deletion in strain K1777 which eliminated traH activity similarly had no effect on F-pilin synthesis. Membranes from three other TraF+ TraH- deletion strains, as well as membranes from all strains carrying deletions extending into traF or further, lacked F-pilin, however. Since traH amber mutations do not affect synthesis of membrane pilin (Moore et al. 1981 b) we conclude that a gene required for F-pilin biosynthesis is located between traF and traH. We have named this gene traQ. Further evidence for traQ and an assay for its activity was obtained by examining the products of a TraM+ TraJ+ TraA+ lambda transducing phage, KI lambda 13, in UV irradiated cells. Infection of F- cells with KI lambda 13 does not result in F-pilin synthesis. Membrane pilin is synthesized as a product of the transducing phage if an Flac or Hfr irradiated host is used, however. Mutant analysis demonstrated that this synthesis is independent of host expression of traA, traL, traE, traK, traB, traV, traW, traC, traU, traF, or traH, but dependent on expression of the traF-traH region. We interpret our data to indicate that an activity encoded by traQ is required for the conversion of traA product to F-pilin.

Bacteriophage lambda↗
PubMed1982

Stability of the canine shoulder joint: an in vitro analysis.

The stability of the canine shoulder joint was assessed with and without the restraining effects of selected supporting structures. Shoulder joints from medium-sized (15 to 30 kg) canine cadavers were dissected, and all soft tissues were removed except for the 4 cuff muscles (supraspinous, infraspinous, teres minor, subscapular), the biceps muscle (m biceps brachii) and the joint capsule with its associated glenohumeral ligaments. The humerus was fixed in position, and with the shoulder extended, the joint was stressed into abduction, adduction, internal rotation, and external rotation. The range of motion was measured from transverse pins placed above and below the joint. Measurements were made before and after severance of the cuff tendons and joint capsule. In selected joints, medial and lateral luxations were created. The biceps tendon was then transposed medially or laterally, respectively, to aid joint stability. Selected medial luxations were stabilized by transferring a portion of the insertion of the supraspinous tendon to a medial location. All joints were stressed as just described, and the range of motion was measured before and after tendon transfer. The joint capsule with its associated glenohumeral ligaments was an important restraint of joint motion. Lateral transfer of the biceps tendon partially corrected lateral joint instability by imposed marked rotational constraints on the joint. Medial transfer of the biceps tendon or the supraspinous tendon partially corrected medial joint instability, but did not resolve rotational abnormalities.

Animals↗
PubMed1981

The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.

We have determined the nucleotide sequence of the rpoD gene which codes for the sigma subunit of RNA polymerase from E. coli K12. The gene, which we formerly cloned as a HindIII restriction fragment in the transducing phage, charon 25, was recloned into several plasmids. We have determined a 2600 base pair DNA sequence which includes the entire structural gene for sigma. The resulting amino acid sequence agrees with previous information obtained about sigma including the amino acid composition, partial sequence data for the N-terminus, the highly acidic nature of the polypeptide, and the cleavage pattern at cysteines. The molecular weight of 70,263 daltons calculated for the 613 amino acid polypeptides is significantly lower than had been determined previously by SDS polyacrylamide gel analysis.

Base Composition↗