PubMed Health⌕ Search

Biomedical subjects

D R Osborne

Publications and source records attributed to D R Osborne.

At least 19 recordsLinked to original sources

Fundamentals of pair diffusion in kinematic simulations of turbulence.

We demonstrate that kinematic simulation (KS) of three-dimensional homogeneous turbulence produces fluid element pair statistics in agreement with the predictions of L F. Richardson [Proc. R. Soc. London, Ser. A 110, 709 (1926)] even though KS lacks explicit modeling of turbulent sweeping of small eddies by large ones. This scaling is most clearly evident in the turbulent diffusivity's dependence on rms pair separation and, to a lesser extent, on the pair's travel time statistics. It is also shown that kinematic simulation generates a probability density function of pair separation which is in good agreement with recent theory [S. Goto and J. C. Vassilicos, New J. Phys. 6, 65 (2004)] and with the scaling of the rms pair separation predicted by L. F. Richardson [Proc. R. Soc. London, Ser. A 110, 709 (1926)]. Finally, the statistical persistence hypothesis (SPH) is formulated mathematically and its validity tested in KS. This formulation introduces the concept of stagnation point velocities and relates these to fluid accelerations. The scaling of accelerations found in kinematic simulation supports the SPH, even though KS does not generate a Kolmogorov scaling for the acceleration variance (except for a specific case and a limited range of outer to inner length-scale ratios). An argument is then presented that suggests that the stagnation points in homogeneous isotropic turbulence are on average long-lived.

Journal Article↗

Acceleration statistics as measures of statistical persistence of streamlines in isotropic turbulence.

We introduce the velocity Vs of stagnation points as a means to characterize and measure statistical persistence of streamlines. Using theoretical arguments, direct numerical simulations (DNS), and kinematic simulations (KS) of three-dimensional isotropic turbulence for different ratios of inner to outer length scales L/eta of the self-similar range, we show that a frame exists where the average Vs = 0 , that the rms values of acceleration, turbulent fluid velocity, and Vs are related by La'/u'2 approximately (V's/u')(L/eta)(2/3+q) , and that V's/u' approximately (L/eta)q with q = -1/3 in Kolmogorov turbulence, q = -1/6 in current DNS, and q = 0 in our KS. The statistical persistence hypothesis is closely related to the Tennekes sweeping hypothesis.

Journal Article↗

Role of breast magnetic resonance imaging in difficult diagnostic situations.

In some patients with breast disease, mammography and ultrasonography can provide only limited diagnostic information. Magnetic resonance imaging of the breast has high sensitivity and specificity and can play a significant diagnostic role in problem situations. Patients who are most likely to benefit are those with (i) axillary adenopathy of unknown primary origin, (ii) possible tumour recurrence after surgery or radiotherapy, (iii) lesions overlying implants, or (iv) those requiring staging of lobular or multifocal carcinoma.

Adult↗

Cat-scratch disease with paravertebral mass and osteomyelitis.

The case of a 9-year-old girl with cat-scratch disease (CSD) complicated by development of a paravertebral mass and osteomyelitis is presented. Following multiple scratches and inguinal lymphadenopathy, she developed back pain, and imaging demonstrated a paravertebral mass with evidence of osteomyelitis involving vertebra T9. The diagnosis was made on the basis of detection of Bartonella henselae by use of molecular techniques on an aspirate from the vertebral column and supportive serology for infection with B. henselae. Eleven other cases of this unusual manifestation associated with CSD have been reported in the literature and are reviewed. The patient was treated with gentamicin, followed by rifampicin and trimethoprim-sulfamethoxazole, orally and made a favorable recovery over 7 months. This is comparable with other case reports, regardless of the choice of antibiotic therapy. CSD in immunocompetent hosts is not always self-limiting, and tissues beyond the lymph nodes can be involved.

Bartonella henselae↗

Determination of ML-1035 enantiomers in plasma by chiral high-performance liquid chromatography.

ML-1035, is a gastroprokinetic agent structurally related to metoclopramide. Because ML-1035 contains an asymmetric chiral sulphoxide moiety, a chiral HPLC method has been developed to separate and quantitate its R- and S-enantiomers in plasma. The ML-1035 enantiomers present in plasma are extracted with dichloroethane under alkaline conditions, the extract evaporated to dryness and reconstituted in the mobile phase. Samples are chromatographed on a Chiralcel OD HPLC column with hexane-absolute ethanol (1% TEA) (1:1, v/v) as the mobile phase. The enantiomers of the unchanged drug are determined by fluorescence measurement (ex: 310 nm, em: 350 nm). The method provides a linear response for both enantiomers over a concentration range of 25 (limit of determination) to 2500 ng ml-1 with correlation coefficients of 0.9987 or greater. The inter-assay precision is 9.5% or less and the accuracy ranges from 93.9 to 103.4% of the theoretical value. The method is used to determine the plasma concentrations of the R- and S-enantiomers following oral and intravenous administration of R- or S-enantiomers to dogs. The method is also adapted to measure enantiomer levels from in vitro reaction mixtures so that the possibility of metabolic inversion may be assessed. The data suggest that no significant level of inversion between the enantiomers occurred either in vivo or in vitro.

Animals↗

Column-switching high-performance liquid chromatographic (HPLC) determination of hydrochlorothiazide in rat, dog, and human plasma.

A fully automated HPLC assay for hydrochlorothiazide in plasma has been developed using a column-switching technique. The method involves direct injection of plasma to the extraction column for sample cleanup followed by switching onto the analytical column. Good precision, accuracy, and linearity were obtained over a range of 25 to 2000 ng/ml in rat, dog, and human plasma. The column-switching method has also been validated by comparison with a conventional HPLC method requiring a cumbersome plasma extraction procedure. Since the method is simple, rapid, and reproducible, it is useful for determination of hydrochlorothiazide levels in animal and human plasma.

Animals↗

Involvement of posterior visual pathways by optic nerve gliomas.

We describe the computed tomographic (CT) findings in seven children with optic nerve gliomas extending posterior to the chiasm. These tumors spread along the optic tracts and in five of seven cases, involved the lateral geniculate bodies and adjacent optic radiations. The propensity for these tumors to extend along both the anterior and posterior visual pathways aids in their diagnosis. An MR scan was obtained in one patient and identified an unexpected site of tumor extension as well as the mass seen by CT.

Child↗

Craniocerebral trauma in the child abuse syndrome: radiological observations.

Experience with craniocerebral trauma in 712 physically abused children is reviewed. Ninety-three (13%) had evidence of head trauma (cranial and/or intracranial). Seventy-seven of these patients had computed tomography (CT) of the head, and 47 had CT evidence of intracranial injury. Extracerebral fluid collections, predominantly convexity subdural hemorrhage, were the most common acute intracranial lesions. Concurrent intracranial and skeletal trauma (cranial and/or extracranial) was present in 33 of the 47 patients (70%) with intracranial injury. A high incidence of skull fractures (45%) in those children with intracranial lesions suggest a significant role for impact head injuries ("battering") in the pathogenesis of craniocerebral trauma in the child abuse syndrome. Greater emphasis on CT examination in evaluation of the abuse infant and child is recommended.

Acute Disease↗

Suprasellar papillary squamous epithelioma ("papillary craniopharyngioma").

Six cases of a distinctive suprasellar papillary neoplasm are presented and the features of this lesion are contrasted with those of the classical adamantinomatous craniopharyngioma. Characteristics distinguishing the former from the latter include a predominate, and perhaps exclusive, occurrence in adulthood; frequent radiologic solidity and absence of calcification; macroscopic papillary nature; and, microscopically, a well-differentiated papillary squamous epithelium without calcification, palisaded cells, or keratoid nodules. We suggest that this papillary lesion is a clinicopathologic entity distinct from the classical adamantinomatous craniopharyngioma.

Adolescent↗

CT demonstration of hyperdense cerebral vasculature due to bromide therapy.

An increase in the effective atomic number of blood is among the factors allowing or influencing the visualization of the major cerebral vessels on noncontrast CT. Bromine (atomic number, 50) attenuates 70 keV X-rays significantly and was responsible for a striking enhancement of the major cerebral vessels on the noncontrast CT in a child being treated for epilepsy. Although oral bromides are only occasionally used in the treatment of epilepsy, their use causes an increase in blood density and thus bromides need to be considered along with other etiologies (reviewed) that allow visualization of the major cerebral vessels on noncontrast CT.

Brain↗

Computed tomographic analysis of deformity and dimensional changes in the eyeball.

Computed tomography (CT) was performed in 40 patients with a confirmed ophthalmic diagnosis and a change in the dimensions or configuration of the eyeball. Abnormalities studied included coloboma, microphthalmus, buphthalmos, axial myopia, macrophthalmus, phthisis bulbi, trauma, neoplasm, posterior staphyloma, granuloma, pseudotumor, and surgical scleral banding for retinal detachment. CT findings could be grouped into three categories depending upon whether the eye was small, large, or normal in size, with the findings in each group allowing distinction of most disease processes.

Eye↗

Role of computed tomography in the radiological evaluation of painful radiculopathy after negative myelography: foraminal neural entrapment.

Thirty-five patients with an unremarkable or a negative water-soluble contrast myelogram and a diagnosis of foraminal neural entrapment made or more firmly established by computed tomography (CT) were detected in evaluating 950 patients presenting for myelography. The CT criterion of foraminal neural entrapment was the presence of a mass displacing epidural fat and encroaching on the neural intervertebral foramen or lateral recess so as to compromise an emerging nerve root. The entrapment (confirmed operatively) was due to a laterally prolapsed disc (16 cases), superior articular hypertrophy (4 cases), lateral recess stenosis (3 cases), posterolateral vertebral bone lipping (2 cases), tumors (6 cases), postoperative scarring (2 cases), spondylolisthesis (1 case), and synovial cysts that encroached on the neural foramina (1 case). CT is an important additional investigation in patients with a painful radiculopathy and a negative or equivocal water-soluble contrast myelogram.

Adult↗

Basal cell carcinoma--a review of treatment results with special reference to cryotherapy.

A retrospective study has been undertaken of all head and neck basal cell cancers treated at the Royal Free Hospital between 1976 and 1980 inclusive. Four methods of treatment were used during the period: simple excision with primary closure (n = 43), skin grafting or trans-position flap (n = 19), radiotherapy (n = 99), and cryosurgical ablation (n = 34). The methods were compared by observing the immediate results, the subsequent tumour recurrence rate and the length of follow-up. Statistical comparison of tumour recurrence rate showed no significant difference between treatment groups. Radiotherapy had the lowest recurrence rate (2%), but required an average of 9 out-patient treatment attendances. Excision with primary closure carried the highest recurrence rate (9.3%), whilst excision with defect closure by flap or graft, which needed a mean in-patient stay of 8.2 days, and cryosurgical treatment had similar recurrence rates of 5 to 6%. Comparing the modes of treatment, cryosurgery was quickest to perform, required no hospitalization and less than 2 out-patient treatment sessions per patient and gave the best cosmetic result. It appears to be a safe technique for treating basal cell cancers and may prove to be the most cost effective method.

Aged↗

Detection of pulmonary emboli in dogs: comparison of single photon emission computed tomography, gamma camera imaging, and angiography.

Single photon emission computed tomography (SPECT) allows separation of radionuclide activity in front of and behind the area of interest and results in more contrast than can be achieved using conventional gamma camera imaging. To determine if this improved contrast is of value in the detection of perfusion abnormalities in the lung, the lower lobe segmental pulmonary arteries of six dogs were embolized and pulmonary perfusion was evaluated using SPECT, conventional gamma camera imaging, and angiography. Although selective segmental angiography was the most sensitive method of detecting emboli, SPECT was much more sensitive than the gamma camera examination in evaluating the effects of the emboli at two hours and one, two, and eight weeks after embolization.

Angiography↗

Postnatal growth and size of the pulmonary acinus and secondary lobule in man.

The acinus and the secondary lobule are important anatomic, functional, and pathologic units in the human lung; there are, however, few studies that specifically describe their postnatal growth and size. Normal lungs from 15 children and adults dying without evidence of pulmonary disease were fixed and dried using a standardized technique and were radiographed after tantalum or silver nitrate bronchoacinography. The acini and secondary lobules were identified and the diameters of these terminal respiratory units determined. The mean diameter of the pulmonary acinus at 1 month was 1 mm; 1 year, 2.5 mm; and at 2 years, 3 mm. The mean acinar diameter had increased to 6 mm at 12 years and reached a normal adult range of 6-10 mm during late adolescence. Acinar size seemed to be related to both height and age. The mean diameter of the secondary lobule at birth measured 3 mm, increased to 5 mm by the end of the first year of life, and by the fourth year measured 9 mm; at 12 years it measured 15 mm, and in late adolescence and adult life it ranged between 13 and 20 mm. A knowledge of the size of the acinus and secondary lobule has a practical application in radiologic practice and will be important in regional structural analysis of the pulmonary parenchyma with computed tomography.

Adolescent↗