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Biomedical subjects

D R Radford

Publications and source records attributed to D R Radford.

14 recordsLinked to original sources
PubMed1997

The effects of surface machining on heat cured acrylic resin and two soft denture base materials: a scanning electron microscope and confocal microscope evaluation.

STATEMENT OF PROBLEM: Rough surfaces promote the adhesion and colonization of denture plaque, therefore it is important to know the character of the surface left by rotary instruments on denture base materials. As a single microscopic technique can introduce artifact, complementary microscope techniques need to be used. PURPOSE: This study investigated the surface characteristics of the machined surfaces of heat-cured acrylic resin, Molloplast B and Novus. MATERIAL AND METHODS: Scanning electron microscopy and confocal microscopy were used to investigate surfaces that had been prepared with appropriate clinical rotary instruments. Thirty samples of each material were prepared with steel and tungsten carbide burs, Molloplast stones, and Arbor bands. An analysis of variance, with the Scheffe multiple comparison of means test, was used to compare average surface roughness of the individual surfaces. RESULTS: The tungsten carbide bur produced a smoother nongrooved surface than the steel bur on acrylic resin. The roughest surfaces were produced on the soft lining materials by the Arbor band and Molloplast stone. CONCLUSIONS: The confocal microscope is a valuable complementary instrument to scanning electron microscopy and provides surface roughness data by using a noncontact method. Each type of rotary instrument produces its own characteristic surface on denture base materials.

Acrylic Resins↗
PubMed1997

Scanning electron microscopy of the development of structured aerial mycelia and satellite colonies of phenotypically switched Candida albicans.

Candida albicans is an asexual diploid fungus that can express high frequency phenotypic switching. Switched variants can develop structured aerial mycelia (SAM) and cultures that have been grown for a protracted period exhibit satellite colonies. This study investigated the development of both SAM and satellite colonies by means of a freeze-drying technique and scanning electron microscopy. The results show that SAM may develop due to hyphae developing in the colony at the base of the structures. The development of satellite colonies is a result of cells that have grown deep into the agar re-emerging on to the agar/air surface and producing a new colony. Although both SAM and satellite colonies are often seen together on mature colonies, their cause and development are different.

Candida albicans↗
PubMed1994

A distance learning programme: 'managing health services'.

Management education is important in general dental practice, community and hospital dentistry. Such education can be acquired via open learning. The distance learning programme, the Managing Health Services Course, offered by The Open Business School of the Open University is recognised by the Institute of Health Services Management and the Committee of Regional Advisers in General Medical Practice for England. The course material, contemporary teaching methods, the level of commitment and the eventual rewards are discussed in this article.

Administrative Personnel↗
PubMed1994

A scanning electronmicroscopy investigation of the structure of colonies of different morphologies produced by phenotypic switching of Candida albicans.

Variant colony formation by Candida albicans has been described and the phenomenon of phenotypic switching has been studied extensively. Whereas the microstructure of non-variant colonies has been investigated by scanning electronmicroscopy (SEM), the relationship between switched variant colonies and microstructure has not been described. The object of this study was to investigate and compared by SEM the microstructure of the normal colony type and five common variant colony types of C. albicans and to determine whether a pattern of dimorphic growth could account for the characteristic colony morphologies. A general relationship between colony type and structure was observed; smooth colonies consisted entirely of blastospores whereas regular, irregular-wrinkled and semi-rough colonies consisted of different proportion of true hyphae and blastospores. Regular extreme-jagged shaped colonies consisted of an almost pure culture of pseudohyphae, and colonies producing aerial hyphae were composed of pseudo- and true hyphae, as well as blastospores. These results show a clear relationship between colony morphology and development of particular cell types.

Candida albicans↗
PubMed1993

A SEM study of denture plaque and oral mucosa of denture-related stomatitis.

The aim of this study was to compare the morphology of denture plaque and adjacent palatal mucosa in five subjects with denture-related stomatitis and a control group of five healthy subjects. Scanning electron microscope examination of copper plate replicas, which preliminary studies established were superior to epoxy resin replicas, showed that there were no consistent differences between the morphology of denture plaque and adjacent mucosa in the denture-related stomatitis group and control group. In each, the surface of the denture plaque was composed predominantly of coccoid- and rod-shaped bacteria with only some yeast cells. The mucosa was largely devoid of microorganisms and individual epithelial cells were shedding from its surface.

Bacteria↗
PubMed1993

Copper plate replica SEM of developing dental plaque in monkeys (Macaca fascicularis).

The aim of the study was to examine the development of dental plaque in macaque monkeys (Macaca fascicularis). Copper plate replicas were constructed from impressions of the labial surface of one of the upper central incisor teeth after tooth cleaning and when plaque had accumulated for 12, 24 and 48 h in each of five animals. Scanning electron microscope examination of the replicas showed that bacteria were present on the tooth surface as scattered individual cells, which after 24 h had formed a continuous layer. Both coccoid and filamentous bacteria were visible in 48-hour-old plaque. Such a rapid accumulation of potentially periodontopathic dental plaque emphasizes the importance of regular and meticulous oral hygiene.

Animals↗
PubMed1991

A two part hinged partial denture design for lower Kennedy Class 4 cases.

It is often difficult to provide patients who have lost lower anterior teeth with a retentive and aesthetic restoration, particularly if the molar and premolar teeth are lingually tilted. A two part hinged denture fully engaging all lingual undercuts for retention provides a useful extension to recognised partial denture design practice.

Denture Design↗
PubMedDate not supplied

A variation in minor connector design for partial dentures.

Whenever possible, the minor connector of a removable partial denture is located in the interdental embrasure. Recent evidence suggests that gingival attachment loss is more probable on the proximal aspect, thus minor connectors located in embrasures cover susceptible sites. A rationale and design are presented for locating the minor connector on the lingual aspect of the abutment tooth rather than in the interproximal area.

Dental Abutments↗
PubMedDate not supplied

The surface of investments poured against different duplicating media.

Two phosphate-bonded investments that were poured into molds of a duplicating gel and a poly(vinyl siloxane) duplicating medium were examined for surface hardness. Untreated surfaces were also examined by the confocal microscope and the scanning electron microscope. The Brinell hardness of samples poured in poly(vinyl siloxane) was greater than that of the same investment poured in duplicating gel. Investments were hardest as set and were significantly softened by drying. Cast hardeners achieved some rehardening of the dried investment samples. Microscopic examination of the investment surfaces revealed greater irregularity of samples poured in duplicating gel than in poly(vinyl siloxane), particularly in one investment.

Agar↗
PubMedDate not supplied

Scanning electron microscopy and energy dispersive analysis of machined denture base surfaces.

To relate the characteristics of rotary instruments to the surfaces they produce, acrylic resin, Molloplast B, and Novus were investigated with energy dispersive analysis and scanning electron microscopy (secondary and backscatter images) before and after machining. The chemical composition of cutting instruments, material surfaces, and residues was identified. Machined debris embedded in Molloplast B after machining with the Molloplast stone was found to contain a mean lead content of 45%. High concentrations of barium sulphate were discovered on the arbor band-machined surface of Novus. These differences were related to clinically appropriate instrumentation, and, therefore, biocompatibility studies that intimately relate to the in vivo situation should be considered for new materials.

Dental Polishing↗