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D Talbot

Publications and source records attributed to D Talbot.

At least 181 records · Page 10Linked to original sources
PubMed1988

Immunoprecipitation of human adrenal microsomal antigen.

Human adrenal microsomes have been labelled with 125I and immunoprecipitated with sera from patients with Addison's disease. The immunoprecipitates were then analysed by SDS-PAGE and autoradiography. 13 of the 23 sera from the Addison patients studied contained antibodies which reacted with a 55 kDa adrenal microsomal protein. The same 13 sera were also positive for adrenal antibodies as judged by immunofluorescence. The 55 kDa protein was not immunoprecipitated from placenta or thyroid microsomes by Addison sera. Furthermore, patients with Graves' disease or rheumatoid arthritis did not immunoprecipitate the 55 kDa protein from adrenal microsomes. Our studies suggest therefore that Addison sera contain antibodies to a 55 kDa adrenal specific protein which may well be the antigen observed on immunofluorescence.

Addison Disease↗
PubMed1988

Aviation gasoline: comparative subchronic nephrotoxicity study in the male rat.

Biochemical and histopathologic parameters of nephrotoxicity were measured in groups of male Fischer-344 rats after a 2-week, 5-days-a-week schedule of oral administration (0.5 ml/kg) of the following substances: aviation gasoline (grade 100) (AVG), automobile regular unleaded gasoline (ULG) and 2,2,4-trimethylpentane (TMP). Results of renal histopathologic examinations and biochemical parameters were compatible with the following order of increasing nephrotoxicity: ULG less than TMP less than AVG. The high nephrotoxic potential of aviation gasoline may be related to its elevated content in branched hydrocarbons.

Acetylglucosaminidase↗
PubMed1987

Evidence for tumour necrosis factor/cachectin production in cancer.

Labile tumour-necrosis-factor-like (TNF) activity was detected by means of an enzyme-linked immunosorbent assay in 50% of 226 freshly obtained serum samples from cancer patients with active disease. In contrast, only 3% of 32 samples from normal subjects and 18% of 39 samples from cancer patients with no clinically evident disease were positive for this factor, with low levels of activity. Greater proportions of serum samples from patients with ovarian or oat-cell carcinoma were positive (69% and 63%) than those from patients with lymphoma (26%). RNA preparations from peripheral-blood mononuclear cells and solid tumours were probed with TNF complementary DNA; evidence of TNF messenger RNA was found in 8 of 11 samples of peripheral-blood mononuclear cells from cancer patients, but only 1 of 8 normal subjects, and in 2 of 6 colorectal tumours. As yet the inducing stimulus and the clinical significance of TNF production in cancer are not understood.

Adult↗
PubMed1987

A rapid, objective method for the detection of lymphocytotoxic antibodies using flow cytometry.

Whilst several centres have reported lymphocytotoxic antibody detection using single and dual fluorescent stains with analysis of the fluorescence emitted from the cell population present in a well of a multiwell plate, problems are encountered with cell concentration and light emission overlap. A method we have developed using flow cytometry produces similar values of percentage cell death using single or double staining techniques (correlation coefficient = 0.9896). This method is not influenced by slight variation in cell number or light emission overlap. The effect of introducing red cell impurities into the normal lymphocyte preparation is described.

Antilymphocyte Serum↗
PubMed1987

Urinary excretion of mercapturic acids and thiocyanate in rats exposed to acrylonitrile: influence of dose and route of administration.

Adult male Sprague-Dawley rats were exposed acutely to acrylonitrile (ACN) according to 1 of 3 different routes of administration: inhalation (6 h), i.v., or i.p. Urinary metabolites measured 24 h after administration were 2-cyanoethylmercapturic acid (CMA), 2-hydroxyethylmercapturic acid (HMA) and thiocyanate (TCN). In all 3 series of experiments, the relationship between excretion of total urinary metabolites and the degree of exposure was reasonably linear. However, there was a marked influence of the route of administration on the pattern of metabolic excretion. For example, after i.p. and i.v. injection CMA was the most important metabolite while after inhalation it was TCN. Our results also indicate an important effect of the dose on the pattern of excretion for all types of administration.

Acetylcysteine↗
PubMed1986

Dipping risk.

Explore the source record for details and available documents.

Animals↗
PubMed1983

Evaluation of the Phadebact coagglutination test for the rapid serotyping of Haemophilus influenza.

Conventional tests for the indentification of Haemophilus species require 24-48 h for completion. In this study, we evaluated the Phadebact coagglutination method for the serotyping of Haemophilus that yields results within 2 min. Of the 150 strains tested, 148 were H. influenzae and 2 were H. parainfluenzae. Of the 83 H. influenzae isolates from cerebrospinal fluid and blood 80 were correctly typed as b by the Phadebact test. Of the 51 sputum isolates, 48 (94%) were nontypable. Eleven (69%) of the remaining sixteen isolates from secondary and unknown sources were type b, four (25%) were nontypable, and one (6%) was in the acdef group. Thirty gram-positive and gram-negative bacteria other than Haemophilus were also tested with no false-positive reactions. The Phadebact test is rapid and easy to perform and interpret.

Agglutination Tests↗
PubMedDate not supplied

Influence of removal from occupational lead exposure on blood and saliva lead concentrations.

Samples of total blood and unstimulated mixed saliva were obtained from 5 male workers occupationally exposed to lead at various time intervals after removal from their work environment. Initial blood lead concentrations were elevated in all workers and then slowly decreased upon removal. Lead concentrations in saliva fell much more abruptly than those in blood, the saliva half-lives being estimated at 5-7 days. Temporary return to work in 2 workers resulted in relatively marked increases of salivary lead concentrations. These results suggest that salivary lead is closely related to recent lead exposure.

Environmental Exposure↗
PubMedDate not supplied

Factors predicting duration of delayed graft function in non-heart-beating donor kidney transplantation.

Non-heart-beating donors (NHBDs) are an important potential source of donor organs, but kidneys from such donors are prone to delayed graft function (DGF) and primary nonfunction, which are multifactorial in origin but believed to be mainly due to warm ischemic injury. This retrospective study examined a series of 88 transplants from Maastricht category II and III NHBDs to examine the role of factors to predict the duration of DGF. The main factors affecting duration of DGF were total warm ischemic time, cold ischemic time, product of perfusate GST concentration and donor age, quality of postoperative graft perfusion, incidence of acute rejection, recipient cardiovascular risk score, maximum pressure on machine perfusion, and weight gain during machine perfusion. Primary nonfunction was not accurately predicted from these factors for kidneys that had passed the viability assessment.

Age Factors↗