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Biomedical subjects

D Tessmann

Publications and source records attributed to D Tessmann.

At least 19 recordsLinked to original sources
PubMed1996

Analysis of connexin43 gene expression induced by retinoic acid in F9 teratocarcinoma cells.

All-trans retinoic acid (10(-7) M) induces cell-cell communication and expression of the gap junction protein connexin43 in mouse F9 teratocarcinoma cells. Northern blot analysis revealed an increase of connexin43 mRNA after treatment with retinoic acid, accompanied by an increase of the mRNA of collagen IV, a differentiation marker. To address the question at what level gene expression is enhanced by retinoic acid, nuclear run-on experiments were carried out. There was no detectable change in the level of newly transcribed connexin43 mRNA. Therefore, we postulate a post-transcriptional mechanism responsible for the regulation of connexin43 mRNA levels by retinoic acid.

Actins↗
PubMed1996

Cloning, expression, and chromosomal localization of the rat mitochondrial capsule selenoprotein gene (MCS): the reading frame does not contain potential UGA selenocysteine codons.

The mitochondrial capsule selenoprotein (MCS) is a selenium-containing polypeptide. It is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. In this paper, we report the isolation and characterization of the rat MCS cDNA and gene. The cDNA contains a reading frame for a 145-amino-acid protein and it lacks the UGA codons, which have been found in the reading frame of the mouse MCS cDNA and have been presumed to encode the selenocysteine in the amino terminal of the deduced mouse amino acid sequence. The deduced amino acid sequence of the rat and mouse MCS shows a high level of homology (79%). The rat MCS gene contains two exons; the intron sequence interrupts the 5' untranslated sequence at the same position as in the mouse MCS gene. The transcription start site is located 184 bp upstream of the translation start site. Alignment of the 5'-flanking regions of the mouse and rat genes reveals that the first 400 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 73%. This conserved region contains no TATA or CAAT box motifs. Northern blot analysis indicates that the MCS mRNA is detectable only in the testis after day 30 of postnatal development. Moreover, in situ hybridization revealed that the rat MCS gene is mainly expressed in round spermatids. From the analysis of mouse-rat cell hybrids that segregate rat chromosomes, the MCS gene was assigned to rat chromosome 2.

Amino Acid Sequence↗
PubMed1993

[Autopsy-confirmed nosocomial infections among the deaths in an intensive care unit].

In a 6-year retrospective study the occurrence of autopsy-confirmed nosocomial infections in 282 deceased patients of an intensive care unit was investigated. The autopsy rate was 100%. In 107 deceased (37.9%) 126 nosocomial infections were confirmed. The most common nosocomial infection was bronchopneumonia (54%). The main ailment was non-tumorous diseases of the digestive system (43.9%). In nearly 60% of the 107 deaths, nosocomial infections were the immediate cause of death. In all 282 deceased, hospital infections were the direct cause of death in 22.7% of these cases. The study emphasizes the importance of nosocomial infections as a causal or contributory factor in the death of patients in an intensive care unit.

Cross Infection↗
PubMed1992

Nucleotide sequence and exon-intron structure of the bovine transition protein 1 gene.

The nucleotide sequence and exon-intron structure of the bovine transition protein 1 gene was determined. It consists of 2 exons (E1, 139 bp; E2, 29 bp) and a single intron (220 bp). The position of the transcription initiation site was determined 30 nucleotides upstream of ATG. TAAATA- and CAAT-boxes were found 60 and 121 bp upstream of the ATG-translation start point, respectively. It was observed that transition protein 1 is highly conserved in mammals at the nucleotide as well as at the amino acid level.

Amino Acid Sequence↗
PubMed1991

[Tangier disease--a "free radical"-associated disease. Results of HDL and anti-oxidant therapy with selenium and D-alpha tocopherol].

In a 26-year-old patient there have been benign enlargements of the lymphatic nodes and a splenomegaly since the end of the adolescence. In the 21st year of age the diagnosis of a Tangier disease was made. Allogenic HDL-rich serum fraction (COHN IV/1-fraction, prepared according to the modified method 6) infused under therapeutic aspect led to a prolonged increase of the serum total cholesterol and of the thrombocytes. The results pled for an activation of the reverse cholesterol transport. Excessively high malonic dialdehyde concentrations in the serum were relating to a "free radical"-associated metabolic defect, which was caused by the hypocholesterolaemia, the reduced transport capacity of vitamin E in the plasma and the nutrition poor in selenium and cholesterol, respectively. Under a nutritive antioxidant supplementation with sodium selenite and D-alpha-tocopherol a slight increase of the total cholesterol, of the thrombocytes as well as a normalization of the MDA values could be reached. The chronic oxidative stress appeared in the patient in a distinct lipofuscinosis of the skin and formations of naevus-cell naevi as an expression of massive denaturations of protein-lipids. In the Tangier disease we must reckon with an increased mutagenic effect of free radicals with an additional DNS repair capacity as well as an increased sensitivity to radical-generating cancerogenic xenobiotics.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1991

[Analgesic nephropathy (AN) with special reference to capillary sclerosis. A multicenter study from the former East Germany].

The incidence of capillary sclerosis in the mucosa of the upper urinary tract was beyond expectation, according to a multicenter study conducted at 11 pathological institutes in all regions of the former GDR from which the following more specific findings were obtained: Capillary sclerosis was recorded primarily from women (1.4:1) in 3.6% of 3,929 autopsy cases (minimum age being 40 years). This result has close to the outcome of a study conducted in Basle, Switzerland, though highest severity (0.33%) and the complete morphological picture of analgesic nephropathy (0.54%) were clearly less frequent, as compared to the above Swiss findings. No reliable conclusion can as yet be drawn regarding the infrequent case of renal pelvis carcinoma. Epidemiological and clinical studies are likely to suggest that an increase in findings might be expected in this part of Germany and might be aetiologically attributable to abuse of analgesics.

Adult↗
PubMed1990

Electrostimulation of healing abdominal incisional hernias by low frequency, bipolar, symmetrical rectangular pulses. An experimental study.

A total of 107 male Wistar rats had abdominal hernias repaired (3 weeks after they had been artificially induced) by a two layer closure of the abdominal wall--the Mayo technique. The wounds of 56 rats were subjected to constant stimulation by an electric flow field. An implanted stimulation unit provided a low frequency (0.87 Hz), bipolar, symmetrical rectangular pulsed current (+/- 25 microA). A control group were given units that did not transmit current. In 39 of the 51 animals in the control group the muscle margins of the abdominal scars separated by between 1 and 5 mm. The scars of the electrostimulated animals were distinguished histologically by early formation of fibroblasts and deposition of collagen and the rapid maturation and longitudinal alignment of the collagen fibres. 46 of 56 of these scars were not separated. This technique may have a clinical application as adjuvant treatment for relapses of incisional hernias.

Abdominal Muscles↗
PubMed1990

[Bipolar impulses or direct current as an adjuvant treatment of incisional hernia? Comparative experimental animal study].

The electrostimulation of the cicatricial healing in the abdominal wall were tested in 119 Wistar rats by means of bipolar rectangularly pulsed current (0.87 Hz, +/- 25 mu A) and direct current (1 mu A). Doubling of the fascia of an abdominal wall hernia was carried out in three groups of rats. In the first group the operational region was stimulated by rectangularly pulsed current, strong proliferated cicatrices were formed with premature production of fibroblasts and collagenous fibres, fast maturation, and high strength. In the second group it was stimulated by direct current, a less exact longitudinal orientation of the collagenous fibres and a slower cicatricial maturation were shown. The third group applied as a control showed a cicatricial distension up to 5 mm. The electrostimulation of the proliferation of connective tissue and cicatricial healing could be pointed out between different tissues (peritoneum and fascia).

Animals↗
PubMed1989

[Endometrium metastasis of a malignant melanoma of the skin].

There is given a case report from personal observation of a metastasis in the endometrium from a malignant skin melanoma. Difficulties for the differential diagnosis of the tumor can arise during the histo- and cytodiagnosis of adequate currettage and smear. These problems are discussed.

Aged↗
PubMed1988

[Mature cystic teratoma of the uterus].

An account is given of the authors own findings from a mature cystic teratoma in the uterus of a woman aged 33 years. Reference is made to analogous cases reported in earlier literature as well as to the two theories at present on teratoma genesis.

Adult↗
PubMed1988

[Coincidence of chronic pancreatitis and duodenitis].

Biopsies taken from various regions of the duodenal mucosa were investigated with a quantitative histological method with a view to frequency, quantity, and localisation of the duodenitis in clinically proven chronic pancreatitis. The histologic classification of duodenitis was made according to Whitehead et al. Out of 46 patients suffering from a chronic pancreatitis, 19 had a duodenitis grade 1 in the pars superior and pars descendens. A more frequent occurrence of duodenitis with concomitant chronic gastritis or a pathologic condition of the bile duct or a chronic alcohol abuse could not be proved statistically.

Aged↗
PubMed1987

[Duodenitis in stomach diseases--a quantitative histologic study].

The coincidence of gastric diseases and chronic duodenitis has been investigated on biopsies and preparations of stomach and duodenum by a quantitative histologic method. The histologic classification of duodenitis was made according to Whitehead et al. 9 of 40 patients with a chronic atrophic gastritis had a duodenitis grade 1. 10 of 40 patients with a chronic gastric ulcer had a duodenitis grade 1 and one patient grade 2. 14 of 40 patients with a gastric cancer had a duodenitis grade 1. By statistical investigations it has been found out that patients with an ulcer or cancer do not have duodenitis more often than patients with gastritis. A possible duodenitis concerning the investigated gastric diseases is mainly due to the chronic gastritis that also could be proved by all patients suffering from ulcer or cancer.

Aged↗