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Biomedical subjects

D Zhang

Publications and source records attributed to D Zhang.

At least 667 records · Page 37Linked to original sources
PubMed1991

[Methanogens from feces of patients with intestine disorder].

Fecal specimens from 10 colorectal cancer, 1 duodenum diverticulosis and 2 healthy adults were examined. Nine fecal enrichments contained Methanobrevibacter. The percentage of methanogen positive individuals was 69% Methanobrevibacter were isolated from fecal enrichments of 4 colorectal cancer and 1 duodenum diverticulosis. The percentage of breath methane positive individuals and methane production had significantly increased in colorectal cancer patients. It will be hopeful that methane production in breath samples at the end of exhalation will became a rapid, simple and non-penetrative method for monitoring precancerous colonic or rectal carcinogenesis and monitoring the relapse of individuals with carcinosectomy.

Breath Tests↗
PubMed1991

Clinical observations on acupuncture treatment of peripheral facial paralysis aided by infra-red thermography--a preliminary report.

We have carried out clinical observations on 34 patients with peripheral facial paralysis treated by acupuncture therapy prescribed according to selection of treatment regimen on the basis of facial thermogram and temperature. A comparison was made with a control group of 97 patients who received conventional acupuncture therapy only. It was found that 1) The cure rate in the group of selecting acupoints by thermogram (hereinafter referred to as the thermography--aided treatment group) was 67.65%, with a marked improvement rate of 26.40%; while the cure rate of the conventional acupuncture treatment group (hereinafter called the conventional treatment group) was 46.39%, the marked improvement rate being 29.90%, indicating a significant difference in therapeutic efficacy between the two groups (P less than 0.02). 2) The average duration of acupuncture therapy for the thermography aided treatment group was 6.02 weeks, whereas that for the conventional treatment group, 24 weeks. There was also a significant difference between the two groups (P less than 0.01). And 3) During the entire therapeutic course, 25.2 sessions of treatment were given on the average in the thermography--aided treatment group, and 78.8 sessions in the conventional treatment group, showing a very significant difference (P less than 0.001). The present thermography--aided method exhibits advantages over the conventional one in enhancing the cure rate and shortening the duration of treatment, which is worthy to be popularized in clinical practice. And it is also of certain significance in standardization and scientification of acupuncture therapy.

Acupuncture Points↗
PubMed1991

[The thermographic observation of the relationship between the retention of acupuncture needles and the effect of nose temperatures].

This is an observation of the relationship between the retention of acupuncture needles and its effect on nose temperature in seventy-four patients with facial nerve paralysis. The patients were divided into five groups according to the period of retention of needles: no retention, retention for ten, twenty, thirty and fourty minutes. Every group consisted twenty cases. The results showed that (a) the nose temperature lowered after acupuncture immediately; (b) then it rose and reached to the maximum at twenty minutes after acupuncture, then lowered again; (c) for the group of no retention and retention for ten and twenty minutes, the temperature rose again during thirty to fourty minutes after acupuncture, for the group of retention for thirty or fourty minutes, however, without these phenomena; (d) the general tendency was that the change of temperature was bigger but lasting for shorter period in the groups of retention for longer time than that in the groups of retention for shorter time. These results verified that some theories in ancient books about the retention of acupuncture needles are scientific.

Acupuncture Therapy↗
PubMed1990

Direct catecholaminergic innervation of primate spinothalamic tract neurons.

Catecholaminergic axonal varicosities identified by immunocytochemical staining for dopamine-beta-hydroxylase were observed at the light microscopic level apposing the somata of retrogradely labeled spinothalamic tract neurons in the monkey spinal cord. Three retrogradely labeled and two intracellularly labeled spinothalamic neurons were serially sectioned and examined at selected intervals at the electron microscopic level. Electron microscopic study revealed that axonal boutons directly contacted the somata and/or dendrites of lamina I, IV, and V spinothalamic tract neurons. All of the profiles apposing one of the retrogradely labeled lamina I spinothalamic tract neurons were categorized from eight planes of section spaced at 1-micron intervals. Of the 305 profiles counted that were adjacent to this soma, 17 (5.6%) stained positively for dopamine-beta-hydroxylase. Of these 17 appositions, three were followed in serial sections to confirm that they had synaptic thickenings and alignment of vesicles along the membrane contacting the spinothalamic tract soma. Catecholaminergic boutons were observed apposing the somata and dendrites of intracellularly filled STT cells characterized as high threshold and wide dynamic range neurons. These observations clearly indicate a direct innervation of spinothalamic tract neurons by catecholaminergic neurons, providing anatomical data to support previous physiological findings demonstrating that catecholamines modulate nociceptive transmission.

Animals↗
PubMed1990

Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin.

Carboxyfluorescein-labeled brain tubulin has been microinjected into stamen hair cells of Tradescantia, and its distribution during mitosis and cytokinesis was examined using confocal laser scanning fluorescence microscopy. The results show that brain tubulin incorporates into plant microtubules and is utilized throughout mitosis and cytokinesis. Microtubule structures that incorporate brain tubulin include the preprophase band, the perinuclear sheath at late prophase, the kinetochore fibers during prometaphase, metaphase, and anaphase, the interzone spindle during anaphase, and finally the phragmoplast during late anaphase and telophase. All of these microtubule-containing structures and, notably, their transitions from one to another have been observed in single live cells progressing through mitosis and cytokinesis.

Journal Article↗
PubMed1990

Regulation of mRNA expression of 3 beta-hydroxy-5-ene steroid dehydrogenase in porcine granulosa cells in culture: a role for the protein kinase-C pathway.

We studied the effect of the tumor-promoting phorbol ester phorbol 12-myristate 13-acetate (PMA), which activates protein kinase-C, on porcine granulosa cells in culture. PMA as well as cholera toxin, forskolin, and hCG increased cAMP accumulation. PMA further augmented the elevation in cAMP accumulation induced by cholera toxin, forskolin, and hCG. In the same cell culture model, hCG induced a time-dependent increase in the 3 beta-hydroxy-5-ene steroid dehydrogenase (3 beta HSD) mRNA levels with a maximal 3-fold stimulation obtained at 8-16 h of incubation with 1 IU hCG/ml. PMA inhibited the increase in 3 beta HSD mRNA levels induced by hCG in a dose-dependent manner. The phorbol ester also inhibited the increase in 3 beta HSD mRNA levels stimulated by LH as well as cholera toxin and forskolin and the cAMP analogs (Bu)2cAMP and 8-bromo-cAMP. Activation of protein kinase-C by mezerein similarly inhibited hCG stimulation of 3 beta HSD mRNA levels. The present data indicate that activation of the protein kinase-C pathway induces generation of cAMP, but causes a near-complete inhibition of the stimulatory effects of hCG, LH, forskolin, cholera toxin, and cAMP analogs on 3 beta HSD mRNA levels in porcine granulosa cells in culture.

3-Hydroxysteroid Dehydrogenases↗
PubMed1990

[Simultaneous determination of brucine and strychnine in semen Strychni by dual wavelength spectrophotometry and studies on processing principles of semen Strychni].

The paper reports the experimental result of the simultaneous quantitative determination of strychnine (ST) and brucine(BR) in Semen Strychni by dual wavelength spectrophotometry. The result shows that there are some deviations in the official method, because the wavelength combination might not have been chosen rightly and the correct coefficient is inconsistent with the facts. We have made a study of the thermal stability of ST and BR, too. On this basis a baking principle for Semen strychni is spelt out.

Drugs, Chinese Herbal↗
PubMed1990

Role of endothelin in the pathogenesis of hypertension in spontaneously hypertensive and 2 kidneys 1 clip rats.

The plasma endothelin (ET) level in spontaneously hypertensive rats (SHR) and 2K1C hypertension animal models and its vasoconstrictive effect on renal and tail arteries were studied. The results demonstrated that there was no difference in plasma ET level between the hypertensive groups SHR, 2K1C, normotensive control groups Wistar Kyot (WKY) and Sprague Dawley (SD), while the vasoconstrictive effect of ET in SHR was more dominant than in WKY. The EC50 of the renal and tail arteries in SHR (0.8912 +/- 0.1662 x 10(-8) M, 0.6103 +/- 0.0878 x 10(-8) M) was apparently lower than that in WKY (1.77 +/- 0.2991 x 10(-8) M, 1.2267 +/- 0.2502 x 10(-8) M, P less than 0.05), but no difference was found in 2K1C and SD rats. The four groups of animals exhibited no difference of such effects as response to norepine-phrine (NA). The findings suggested that the increased arteriole sensitivity to ET be an important factor in the pathogenesis of hypertension in SHR.

Animals↗
PubMed1990

[The comparison of changes of the facial temperature after acupuncturing point of hand and foot-yangming meridians by the thermography].

With thermographic technique, We observed changes of the facial temperature after acupuncturing Hegu (L14) and Zusanli (S36) in 65 patients (103 cases in all) with facial nerve paralysis. The observation showed that the average of Hegu group was higher than that of Zusanli group. The result explained that the effect of acupuncture at points of hand-yangming meridian was bigger than that of foot-yangming meridian on the facial temperature.

Acupuncture Points↗
PubMed1990

[Objective display on phenomena of propagated sensation along channels (PSC)--changes on the infrared thermal image channels pathway of upper extremity].

This paper reported the observed results on changes of the infrared thermal image of upper extremity induced by needle "Hega" (LI 4), "Daling" (P 7) and "Neiguan" (P6) points. The results indicated that 10 out of 23 cases whose higher and middle temperature bands of upper extremity distributed along the "Dachang" (Large Intestine) channels, when they felt phenomena of propagated sensation along the "Dachang" channels induced by needle "Hegu" points. Those effects did not appear in the objects who did not feel PSC along the "Dachang" channels induced by needled points. Above results provide an objective basis for the phenomena of channels and suggest that the phenomenon of PSC is an objective fact in human body.

Acupuncture Points↗
PubMed1990

[Objective observation on phenomena of sensation along channels (PSC) and QI reaching to affects area (QIRA)--the influence of acupuncture points on infrared thermal image of face].

Thirty three patients and nine volunteers were observed. 18 out of 33 patients felt PSC and Qi reaching to face induced by needle points. The QiRA rate was 80% in patients with face diseases. During acupuncture "Hegu" (LI 4) points, most of the patients felt PSC reaching to their faces and sensed heat in face. The infrared thermal image demonstrated that the temperature of face skin increase and the hyperthermal areas coincided basically with the pathway of "Yangming" channels. Those effects were more than the effects of the other objects who didn't feel PSC or sensed Qi reaching to face induced by needle "Hegu" (LI 4) or "Daling" (P 7) Points. The difference between the two groups was statistical significant. The temperature rising responses induced by needle points were not the results of natural fluctuation. The above results indicate that the PSC and QiRA is objective existence.

Acupuncture Points↗
PubMed1990

Research on the acupuncture principles and meridian phenomena by means of infrared thermography.

In this study, the observation on acupuncture manner, the specificity of acupoints, maintaining needle and "MiuCi" needling, manual and electrical acupuncture, the thermal characteristics of meridians etc. were carried out in order to apply the thermography in the research of principles of acupuncture and meridian phenomena thus create a way to approach to the mechanism of acupuncture effect. Results showed a good prospect for this technique being widely used in the investigation of acupuncture and meridians and its practical significance. It's also identified that the thermography with the advantages of straight-forward, objective and simple fitted to be used in the research of acupuncture and meridians.

Acupuncture Points↗
PubMed1990

[Tumour necrotic factor (TNF) in the pathogenesis of liver necrosis in viral hepatitis and strategy for its prevention and treatment].

We determined serum and peripheral blood mononuclear cell (PBM) TNF activity in 8 healthy donors and 47 patients with hepatitis by using 3H-thymidine release method of Maennel et al. The serum TNF levels were significantly increased in chronic active hepatitis and subacute hepatic failure (13.8 +/- 6.3, and 19.4 +/- 3.9). Patients with serum bilirubin more than 10 mg/ml showed a higher serum TNF level than those with lower serum bilirubin. A significant elevation of TNF level was also observed in patients with positive endotoxemia or concurrent bacterial or viral infections. Experimental liver injury in Wistar rats induced by galactosamine (GLN) and LPS produced marked increase of serum TNF level and submassive liver necrosis. It is noteworthy that normal serum TNF and markedly ameliorated liver injury were observed in rats that received combined treatment with GLN, LPS and hepatopoietin (HPN), a low molecular peptide extracted from suckling porcine liver. In vitro, HPN also significantly suppressed TNF activity when it was co-incubated with PBM and LPS. An encouraging result was observed in preliminary clinical trial of HPN for the treatment of subacute liver failure. It suggests that Serum TNF appears to play an important role in the pathogenesis of liver injury from viral hepatitis and HPN seems to be a protection in liver injury against TNF activity.

Adolescent↗
PubMed1990

[A comparative study of the intelligence of younger children in Guizhou city, the countryside and areas of Miao].

This essay sums up the experience of China-wechsler young child scale of intelligence (C-WYCST) to test the intelligence of the normal younger children living in Guizhou city, the countryside (Han and Miao). Primarily, we make clear the general information concerning the mental development by testing 1057 aged from 3 and 10 months to 6 and 10 months among them. The essay also demonstrates the importance of cultivating the younger children intelligence, especially to further the cultivation of the countries and the minority nationalities younger children in our prorinee.

Adolescent↗