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Despina Deligianni

Publications and source records attributed to Despina Deligianni.

3 recordsLinked to original sources

Biomechanical evaluation of conventional internal contemporary spinal fixation techniques used for stabilization of complete sacroiliac joint separation: a 3-dimensional unilaterally isolated experimental stiffness study.

STUDY DESIGN: Comparative 3-dimensional biomechanical testing. OBJECTIVE: To compare 5 fixation techniques, 3 using screws or screw and plates and 2 spinal, used for stabilization of complete unilateral sacroiliac dislocation in composite models. SUMMARY OF BACKGROUND DATA: Harrington compression rods have been used for posterior iliosacral stabilization. Recently, the use of compact spinal instrumentation has been introduced for stabilization of iliosacral joint separation to achieve immediate and permanent stability, allowing early mobilization. To the authors' knowledge, no comparative mechanical studies between commonly used internal fixation techniques and contemporary spinal instrumentation have been performed. METHODS: Fifteen identical composite models of the left hemipelvis and sacrum were used to simulate consistently the "worst-case scenario" of complete unilateral sacroiliac dislocation. Subgroups of 3 models each were used to apply 5 (A-E) alternative fixation iliosacral joint fixation techniques: 1 multiaxial 7.5 mm Cotrel-Dubousset screw inserted in the posterior superior iliac spine and connected with a long Cotrel-Dubousset horizontal rod with 6.5 mm multiaxial Cotrel-Dubousset screws inserted bilaterally in the S1 pedicles (technique A); 1 multiaxial 7.5 mm Cotrel-Dubousset titanium pedicle screw inserted in the posterior superior iliac spine and connected with a short horizontal Cotrel-Dubousset-rod to a 6.5 mm multiaxial Cotrel-Dubousset-screw inserted to the ipsilateral S1 pedicle (technique B); 1, 6.5 mm cancellous AO-screw (technique C); 2, 6.5 mm cancellous AO screws (technique D); and 2 dynamic stainless steel compression plates (technique E) placed anteriorly. Constructs were biomechanically tested. The ilium was unilaterally rigidly fixed, the sacrum was put horizontal in the mediolateral direction with a forward tilt of 30 degrees (close to physiologic conditions) in the sagittal plane, and a vertical quasi-static compressive load ranging from 0 to 500 N was applied on the endplate of S1, reproducing a "worst case" loading scenario. Construct stiffness, frontal plus sagittal kinematics, and iliosacral joint gap size for all 5 techniques were measured. RESULTS: The construct stiffness (N/mm +/- standard deviation) ranged for model: A, 121 +/- 18; B, 78 +/- 10; C, 168 +/- 13; D, 193 +/- 42; and E, 145 +/- 4. All other parameters exhibited minor variations between the different techniques of fixation: at the 400 N load level, the maximum iliosacral gap globally ranged 0.9-2.8 mm, the maximum mediolateral sacral tilt ranged 1.3-2.4 degrees, and the maximum anteroposterior sacral tilt ranged 0.6-3.0 degrees. CONCLUSIONS: The iliosacral fixation with 2 6.5 mm AO-cancellous screws for complete sacroiliac dislocation demonstrated the highest stiffness and the short spinal instrumentation the poorest stiffness. All other fixation techniques could be generally considered of equivalent stability value.

Biomechanical Phenomena↗

Experimental usage of hydroxyapatite preadsorption with fibronectin to increase permanent stability and longevity of spinal implants.

UNLABELLED: Hydroxyapatite has been used in orthopaedic and particularly in spinal surgery by precoating implants to indirectly increase osteoblasts' adhesion and subsequently their stability and longevity. Fibronectin preadsorption synergistically with appropriately constructed hydroxyapatite's surface texture to enhance osteoblasts' adhesion has not been, to the authors' knowledge, previously investigated. In osteoporotic spines, methods to increase implant stability (pedicle screws and cages) are of major value. OBJECTIVE: This experimental study investigated the contribution of fibronectin preadsorption to enhance osteoblasts' adhesion and strength on hydroxyapatite. METHODS: Hydroxyapatite substrata with two different surface roughnesses (rough HA180 and the smooth HA1200) were produced and human osteoblasts were seeded on them after culture. Prior to osteoblasts seeding, the hydroxyapatite substrata were immersed in fibronectin solution. Osteoblast attachment on each of the two hydroxyapatite substrata was evaluated by recording the number of cells, while the osteoblast's adhesion strength was determined by measuring the shear stress required to detach the cells from the hydroxyapatite substrates. RESULTS: Fibronectin preadsorption increased the number of attached osteoblasts on smooth and rough hydroxyapatite substratum at 40% and 62% respectively, while it increased osteoblast attachment strength on the smooth and rough substratum at 165% and 73% respectively. CONCLUSIONS: Fibronectin preadsorption and smooth hydroxyapatite surface texture synergistically increased the adhesion's strength of human osteoblasts "in vitro", while preadsorption and rough hydroxyapatite surface increased the number of attached osteoblasts. Further studies in primates and human beings should be carried out to disclose the clinical relevance of the above mentioned observations in spine surgery.

Coated Materials, Biocompatible↗

Fibronectin preadsorbed on hydroxyapatite together with rough surface structure increases osteoblasts' adhesion "in vitro": the theoretical usefulness of fibronectin preadsorption on hydroxyapatite to increase permanent stability and longevity in spine implants.

OBJECTIVE: The aim of this study was to investigate the contribution of fibronectin (FN) preadsorption to enhance osteoblast adhesion and strength on hydroxyapatite (HA) used either as osteoconductive bone substitute or precoating of pedicle screws and cages in spine surgery. METHODS: HA substrata with two different surface roughness values (rough HA180 and smooth HA1200) were produced, and human osteoblasts were seeded after culturing on them. Prior to osteoblast seeding, the HA substrata were immersed in FN solution. Osteoblast attachment on each of the two HA substrata was evaluated by recording the number of cells, whereas osteoblast adhesion strength was determined by measuring the shear stress required to detach the cells from the HA substrates. RESULTS: FN preadsorption increased the number of attached osteoblasts on smooth and rough HA substratum at 40% and 62%, respectively, whereas it increased osteoblast attachment strength on the smooth and rough substratum at 165% and 73%, respectively. CONCLUSIONS: This study showed that FN preadsorption and rough HA surface texture synergistically increased "in vitro" both the number and the adhesion strength of human osteoblasts. Further studies in primates and humans should be carried out to disclose the clinical relevance (increase implant's stability and longevity) of the above-mentioned observations.

Adsorption↗