PubMed Health⌕ Search

Biomedical subjects

Duo Wang

Publications and source records attributed to Duo Wang.

21 records · Page 2Linked to original sources

[The distribution of Candida spp. in the oral cavities of normal children].

OBJECTIVE: To study the distribution of Candida spp. mainly Candida albicans in the oral cavities of health children. METHODS: Four groups children of different ages, A1: newborn babies, A2: 3.2 years old (average) children,A3, 7.2 years old,A4:12.7 years old and B control group 20.4 years old,mucosal swab sampling with centrification,CHROMagar Candida identified culture medium for culture and identification, and different methods for isolating Candida albicans for A3 group. RESULTS: The isolation rates of Candida spp were A1 7.5%,A2 70%,A3 56.36%,A4 49.12%,B 27.5%,the proportion of Candida albicans also diversely,and the method of PCR was more sensitive than the one of culture. CONCLUSION: Candida spp can be isolated from the normal oral cavities in different aged children, most of them were Candida albicans,both the isolation rates of Candida spp and the proportion of Candida albicans were different.

Adolescent↗

[A study on the rate of collagenase in the degradation of human root dentin in vitro].

OBJECTIVE: The purpose of this study was to evaluate the effect of degradation of human root dentin collagen treated with collagenase after demineralization. METHODS: The root dentin samples were demineralized by incubating with PH5.0 lactate acid at 37 degrees C. The incubation solutions were taken on days 1, 7, 14, 21 and 28, and centrifuged 10,000r/min at 4 degrees C. The supernature solutions were analyzed for Ca(2+) and hydroxyproline. The demineralized root samples were incubated with PH7.4 collagenase solution for 7 days at 37 degrees C. The incubation solutions were centrifuged 10,000r/min at 4 degrees C and analyzed for hydroxyproline. RESULTS: The release of Ca(2+) increased and had significant differences at all observation periods within 28 days (P<0.05). There were no significant differences of the amount of degradable collagen during the nonenzymatic procedure (P>0.05). The amount of collagenase-degradable collagen was proportional to the calcium released until a plateau value at a calcium loss of about 80.01 micromol/cm(2) was reached. CONCLUSION: The degradability of collagenase is in close association with calcium released. The retention of mineral is of relevance for lesion arrestment or remineralization of early root caries.

English Abstract↗