Biomedical subjects

E A Tkachenko

Publications and source records attributed to E A Tkachenko.

At least 37 records · Page 2Linked to original sources

PubMed1987

Partial characterization of a Hantavirus isolated from a Clethrionomys glareolus captured in Belgium.

A Hantavirus was isolated in Vero-E6 cells from lungs of a free living bank vole (Clethrionomys glareolus) captured in Turnhout, Province of Antwerp--Northern part of Belgium. With help of monoclonal antibodies the Belgian Hantavirus isolate could be clearly differentiated from Hantaan virus strain 76-118, Prospect Hill virus strain PH1 and SR11, a Hantavirus isolated from laboratory Wistar rat in Japan, but not from the nephropathia epidemica virus strain Hällnäs.

Animals↗

PubMed1987

[Clinico-epidemiologic and laboratory research on hemorrhagic fevers in Guinea].

In 1982-1983, were reported the cases of haemorrhagic fevers among populations living in the Madina-Ula district of Guinea. Clinico-epidemiological and serological studies (experimental studies) reveal into presumption of Ebola and Lassa fever viruses significance in the etiology of the disease outbreaks. Antibodies to Ebola virus were recognized in 19% from total number of sweating reconvalescent patients with the same clinical features, in order to 8% in healthy local populations. Antibodies to Lassa virus were detected in 3 cases, in 4 cases was revealed Lassa virus antigen in small rodents.

Antibodies, Viral↗

PubMed1986

[Time periods of antibody preservation in patients convalescing from hemorrhagic fever with renal syndrome in European foci of the infection].

The radioimmunoassay of serum samples from 76 convalescents after hemorrhagic fever with the renal syndrome (HFRS), that took place in 1964 in Ufa, revealed the presence of specific antibodies in 75% of the convalescents. The absence of antibodies may be attributed both to their loss in some of the convalescents and to mistakes in the clinical diagnosis. The study of serum samples from 19 convalescents who had the infection in 1960 during the laboratory outbreak of HFRS at the Gamaleya Institute of Epidemiology and Microbiology (Moscow) showed the presence of antibodies in all convalescents. In both groups the infection was linked with common red-backed voles (the Western serological variant of the virus).

Adult↗

PubMed1986

Hemorrhagic fever with renal syndrome in Czechoslovakia: detection of antigen in small terrestrial mammals and specific serum antibodies in man.

A direct ELISA technique was used for demonstration of HFRS virus antigen in suspensions of the lungs obtained from 774 wild small mammals belonging to 13 zoological species trapped in 21 localities in Czechoslovakia between 1983 and 1984. Western serotype of HFRS virus was demonstrated in the lungs of 8 Microtus arvalis voles caught in the areas of central Bohemia, southern Moravia and western Slovakia, and in one Clethrionomys glareolus vole from the area of eastern Slovakia. The eastern serotype antigen was found in one Apodemus agrarius field mouse captured in the area of eastern Slovakia. The antigen titres as determined in the lungs of these rodents by the ELISA and RIA techniques were relatively high. Human sera examined for the presence of HFRS-specific antibody by the indirect fluorescence and RIA techniques were 76 serum specimens from chronic kidney patients treated by hemodialysis and 349 sera of adult males over 35 years of age coming from various districts of Bohemia and Moravia. Antibodies against HFRS virus antigen were found in 4 sera obtained from northern Bohemian men. The results provide the evidence which suggests that the natural foci of HFRS are likely to occur across much of the Czechoslovak territory, and confirms the focality of this infection in at least some of the localities characteristic in the 1950s and 1960s of the incidence of severe sporadic cases of HFRS.

Adult↗

PubMed1986

[The use of direct immunoenzyme analysis with genus-specific peroxidase conjugate in leptospirosis].

For the first time anti-Patoc I peroxidase conjugate was obtained from the serum of a hyperimmunized guinea pig by the method of Nakane and Kawaoi (1974) in the modification of Mathiesen et al. (1978). In the experimental study of 37 Leptospira strains belonging to 19 serological groups, carried out with the use of direct EIA techniques, anti-Patoc I peroxidase conjugate was found to be genus-specific and highly active. The possibility of detecting Leptospira antigens of 7 serological groups in the blood and in suspensions of the liver and kidneys of golden hamsters by direct EIA with the use of the above-mentioned conjugate was established. The use of direct EIA with anti-Patoc I peroxidase conjugate for the diagnosis of leptospirosis is recommended.

Animals↗

PubMed1986

[Incidence and features of neuroinfections induced by lymphocytic choriomeningitis virus].

A follow-up examination of blood sera and cerebrospinal fluid was carried out in 413 patients with various neuroinfections and related diseases. The modern immunological methods were employed: the complement fixation test, the fluorescent antibody test as well as immuno-enzymic and radioimmunoassays. It was established that 8.5% of serous meningitides and 12% of encephalitides were induced by lymphocytic choriomeningitis (LCM) virus. The verified diseases were subjected to a clinical analysis. It is emphasized that the immunological examination of the cerebrospinal fluid in patients with LCM infection contributes to a more detailed study of the pathogenesis of the disease.

Adolescent↗

PubMed1985

Appearance of antibodies to two viruses in cerebrospinal fluid of patients with aseptic meningitis.

Two cases of aseptic meningitis with simultaneous presence of antibodies to lymphocytic choriomeningitis (LCM) and mumps viruses were observed. In the 1st patient antibodies to both viruses appeared in sera and cerebrospinal fluid (CSF). The 2nd patient displayed rising serum antibodies to both viruses and CSF antibodies to mumps virus. Immunological findings and clinical features of the patients are discussed.

Adolescent↗

PubMed1984

Antibodies to the HFRS virus in the human population of European RSFSR as detected by radioimmunoassay.

Natural immunity to the causative agent of hemorrhagic fever with renal syndrome (HFRS) has first been studied using radioimmunoassay (RIA) in the human population of the Bashkir ASSR with the highest incidence of this infection and of five other regions of the RSFSR with lower incidence of HFRS. The antigen was prepared as a suspension of lungs of rodent from natural HFRS foci and contained a high concentration of virus protein. 12,000 sera from the population of 6 areas of the RSFSR were examined. In the Bashkir ASSR antibodies were detected in 13.7 per cent of the subjects examined, this figure varying in different districts from 4.0 to 41.5 per cent. In the other areas the portion of immune subjects varied from 6.7 per cent in Kuybyshev region to 1.6 per cent in Vladimir region. No correlation between the size of the immune portion of the population and average incidence rates for 5 years was observed. In Bashkiriya, immunity was found in 14.9 per cent of men and 11.8 per cent of women. In other regions, the per cent of women with antibodies to HFRS virus was also lower. In the age-group under 40 the percentage of immunity was lower (11.4 per cent) than in older age-groups (17.4 per cent). The portion of immune subjects varied in different occupation groups. In HFRS convalescents the antibody was found to persist in high titre for 20 years (the observation period).

Adolescent↗

PubMed1984

Indirect solid-phase immunosorbent assay for detection of arenavirus antigens and antibodies.

Indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) using either anti-human or anti-mouse IgG labelled with horseradish peroxidase and 125I, respectively, were developed for the detection of Junin, Machupo, Tacaribe, Amapari, Tamiami, Lassa and LCM arena-viruses. Both methods allow high sensitivity detection of arena-virus antigens and antibodies.

Animals↗

PubMed1984