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Biomedical subjects

E A Tkachenko

Publications and source records attributed to E A Tkachenko.

At least 55 records · Page 3Linked to original sources
PubMed1981

Enzyme-linked immunosorbent assay for detection of arenaviruses.

Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture fluids. It shows higher sensitivity and allows an earlier detection of virus-specific antigens as compared with the CF test.

Animals↗
PubMedDate not supplied

[An epidemiological analysis of hemorrhagic fever with renal syndrome morbidity in the Republic of Bashkortostan in 1997].

The outbreak of hemorrhagic fever with renal syndrome (HFRS) in the Republic of Bashkortostan, resulting in 10,057 registered cases of the disease (287 cases per 100,000 of the population), was analyzed. HFRS cases among the population were registered in 52 out of 54 regions of Bashkortostan. 31% of the total number of patients were the inhabitants of rural regions (170 cases per 100,000) and 69% were urban dwellers (295 cases per 100,000), mainly in Ufa (512 cases per 100,000). HFRS morbidity among males was fourfold higher than among females. In 70% of cases persons aged 20-49 years were affected. 5% of the total number of patients were children aged up to 14 years. In 34 cases (0.4%) the severe clinical course of the disease had a fatal outcome. Cases of HFRS were registered from April 1997 till March 1998 with the highest morbidity rate observed during the period of August-December. In most cases (46.8%) both urban and rural dwellers contacted infection during a short-term stay in the forest. As the result of the serological examination of the patients, all HFRS cases were etiologically attributed to hantavirus, serotype Puumala. The main natural reservoir of this virus and the source of human infection in Bashkortostan were bank voles (Clethrionomys glareolus), the domination species among small mammals in this region.

Adolescent↗
PubMedDate not supplied

[Outbreak of hemorrhagic fever with kidney syndrome in Egor'ev district of Moscow region].

Epizootological, serological, and molecular virological analysis of an outbreak of hemorrhagic fever with renal syndrome (HFRS) in the Egoryevsk district of Moscow region (September 1995-January 1996) has been carried out. Hantavirus (Puumala) antigen and virus-specific antibodies were isolated from bank voles captured in the endemic focus. Anti-Puumala antibodies were detected in the sera of all HFRS patients and in 2% healthy residents of the endemic focus. Analysis of nucleotide sequence (RNA from hantavirus-positive lung of a bank vole) showed that the studied hantavirus is a distinct genotype of Puumala virus. Hence, a new highly active natural focus of HFRS associated with Puumala virus, dangerous for the population, has been revealed in Moscow region.

Animals↗
PubMedDate not supplied

[The spread of hantaviruses in western Siberia].

Studies made in west Siberia established the existence of at least 4 hantavirus types: Puumala, Tula, Topografov, and Dobrava/Belgrade. The authors detected Puumala virus genovariants in voles, Tula virus in sagebrush and narrow-skulled voles, and Topografov virus in Siberian lemmings. The etiological role of the hantavirus Dobrada/Belgrade was defined in the structure of morbidity of hemorrhagic fever with the renal syndrome. In Russia, hantaviruses were first detected in mites: a hantavirus antigen was found in one of the enzyme immunoassayed pools of the mole mites (Haemogamasus ambulans) collected from the Siberian lemming brooding burrow in the near-floodplain habitats of the transitional Yenisei tundras in southwest Taimyr.

Animals↗
PubMedDate not supplied

[Adaptive property of hantaviruses during their isolation from cultured VERO-E6 cells in relation to the type of the ecological host].

As a result of virological studies, 185 lung tissue specimens from 4 rodent species caught near Khabarovsk were isolated and fixed in the passages of cultured Vero-6 cells of 68 hantavirus strains. The capacity of the strains to adapt to the cells was assessed by using the adaptive index involving the mean rates of successful isolation, its duration, hantavirus antigen titer in the material used for infection. The strains of hantavirus serotypes were noted for the highest adaptive properties, which are ecologically associated with rodents of the family Mus, such as field and East-Asiatic mice. Lower adaptive capacities were established for the strains of hantavirus serotypes, which are ecologically related to rodents of the family Cricetidae, such as large and large-toothed redback voles. The differences found in the adaptive capacities of hantavirus strains cultured in Vero-E6 cells reflect the degree of specialization of some hantavirus serotypes to particular host rodent species during their long-term coevolution.

Adaptation, Biological↗
PubMedDate not supplied

[Hantaviruses and hantavirus infections].

Hemorrhagic fever with renal syndrome (HVRS) has been for decades a topical problem for healthcare systems of many countries in the Eurasian continent. Viruses triggering HVRS alongside with other related viruses (but not pathogenic to man) were discovered in 70-80-ies and formed a new genus Hantavirus of the Bunyaviridae family. The study results of a severe outbreak of the respiratory disease with the mortality rate of 60% (South-West of the USA, 1933) showed that hantaviruses were also among the causative agents. Later, the disease was designated as hantavirus cardio-pulmonary syndrome. By now, it has been established that hantaviruses are wide spread with different rodents being their carriers. The discussed viruses cause, in rodents, a chronic asymptomatic infection and are transferred, later, to man by the aerogenic path through excretions of infected animals. Studies of hantaviruses have been restricted for a long time due to their high pathogenicity (protection equipment not below than the P-3 level is needed), because of a lack of a laboratory model of infected animals and because of a low growth in cell cultures. With the rapid development and application of molecular biological techniques of the recent years, substantial progress has been made in studies of hantaviruses. Different aspects of hantavirus ecology, molecular biology, morphology, pathogenesis and diagnostics are discussed in the offered survey.

Animals↗
PubMedDate not supplied

[The epizootological and virological characteristics of a natural hantavirus infection focus in the subtropic zone of the Krasnodarsk Territory].

A natural focus of hantavirus infection was detected and examined during the studies conducted in 2000-2002 around the Sochi (the western spurs of the Great Caucasus Ridge, which descended to the Black Sea (the Krasnodar Territory of Russia). At least 4 rodent species, such as Microtus majori, A. (S.) ponticus, A. agrarius, A. (S.) ciscaucasicus, were shown to participate in the circulation of hantaviruses. A comparative analysis of the nucleotide sequences of genomic S- and M-segments of hantaviruses has provided evidence that 13 viral RNA isolates from the A. (S.) ciscaucasicus belong to the Dobrava/Belgrade virus clade; however the RNA isolate from the Microtus majori belong to the Tula virus clade.

Animals↗
PubMedDate not supplied

[Epizootology of hemorrhagic fever with renal syndrome in the Central Chernozem Region].

A total of 5149 small mammals belonging to 16 species were collected from the Lipetsk, Voronezh, and Belgorod regions (40 administrative districts) in 2003-2004 and examined by ELISA and IFA to detect hantavirus antigen and antibodies in the lung tissues. Hantavirus circulation was revealed in 13 species, the highest hantavirus activity being detected in field (Apodemus agrarius) and small wood (A. (S) uralensis) mice (Dobrava-Belgrad virus), bank (Clethrionomis glareolus) (Puumala virus) and common (Microtus arvalis) (Tula virus) voles. These species were frequently found to have their untypical hantaviruses, which was most commonly observed in small wood mice. It is suggested that the small wood mouse is likely to take a certain part in maintaining the circulation of Dobrava-Belgrad virus.

Animals↗
PubMedDate not supplied

[Characteristics of endemic foci of hemorrhagic fever with renal syndrome in various regions of the USSR].

Clinical, epizootological, and laboratory data for 9 endemic foci of hemorrhagic fever with renal syndrome (HFRS) were compared. The analysis covered 5120 cases of HFRS. It was established that in the foci, where the leading role is played by field mouse and Japanese field mouse, HFRS is caused by the 1st serotype of HFRS virus, and the clinical course of the disease is quite severe. In the natural foci with the predominance of redbacked vole, the 2nd serotype of the virus circulates and the disease is of moderate severity or mild. In each individual case, however, both serotypes of the virus may produce both mild and severe diseases.

Animals↗
PubMedDate not supplied

[Hantaan virus serotypes circulating in foci of the Far Eastern region of the USSR].

Studies on serodifferentiation by indirect immunofluorescence antibody method and neutralization test of Hantaan virus strains isolated from human patients and rodents revealed circulation on the Maritime Territory of 5 virus serotypes and demonstrated that rodents of one species could be virus carriers of different virus serotypes. On the basis of the antigenic similarity of strains isolated from human patients, field mice, and Clethrionomys rufocanus it is concluded that the virus serotypes circulating among rodents of these species may play a role in the etiology of cases of hemorrhagic fever with renal syndrome.

Animals↗
PubMedDate not supplied

[The role of Hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the Far East of the USSR].

The employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of Hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (HFRS). Proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and Cl. rufocanus were obtained. No data on any association of human HFRS cases with strains of serotype 4 isolated from reed voles could be demonstrated.

Animals↗
PubMedDate not supplied

[Virus-specific proteins and RNA of the virus of hemorrhagic fever with renal syndrome].

Virus-specific proteins G1, G2, and N with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in VERO E-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (HFRS) isolated in the European USSR from a patient with HFRS, a fatal human case of HFRS (the strains K-27 and P-360) and from a bank vole (strain CG-1820). The sera from human convalescents after HFRS in the European USSR and rat sera prepared with the CG-1820 strain precipitated proteins possessing similar electrophoretic characteristics from a lysate of cells infected with the CG-1820, K-27 and P-360 strains. The sera from human HFRS convalescents in the Far East did not precipitate protein Gl. The viral RNA derived by immunosorption method from intracellular nucleocapsids of CG-1820 strain and strain 4590 (isolated from Ap. Peninsulae in the Far East) contained 3 classes of molecules: L, M, and S. L- and M-RNA of these strains had the same molecular weight (1.62 and 1.38 megadaltons). The molecular weight of S-RNA of the strain 4590 was 0.76 megadalton and that of the CG-1820 strain 0.83 megadalton. It is assumed that there are species differences among the viruses, causative agents of HFRS, circulating in the European and Far East regions of the USSR.

Animals↗