[Action of gamma rays and fast neutrons on monolayer human bone marrow cultures].
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Biomedical subjects
Publications and source records attributed to E A Zherbin.
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gamma-globulin from rabbit sera containing antibodies to denatured DNA or cytidine suppressed the efficiency of colony formation in agar cultures of human bone marrow cells. The antibodies to DNA isolated from the immune sera by the immunosorbent produced an analogous effect. gamma-globulin from the sera of intact animals and immune sera after the removal of antibodies to denatured DNA from them produced a different effect. gamma-globulin in a concentration of 0.28 and 5 mg failed to influence the colony-formation of 2.10(5) explanted nucleus-containing cells, by stimulated the colony growth when added in a concentration of 15 mg.
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A method of exogenous splenic colonies was applied to the study of the dynamics of the content of the colony-forming units (CFU) in the bone marrow of CBA mice to which thermal burn of the III degree of 15% of the body surface was inflicted. On the 4th and 16th days after the burn the CFU content in the bone marrow of mice decreased 1.7-2.1 times. The thymus cells of the intact mice administered simultaneously with the bone marrow of the burned mice increased, the amount of the splenic exogenous colonies formed in the recipients. The data obtained permitted to make a suggestion that not only the CFU count diminished in the bone marrow in the burned animals, but also the thymus-dependent cells necessary for normal colony formation.
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