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Biomedical subjects

E Behm

Publications and source records attributed to E Behm.

At least 19 recordsLinked to original sources
PubMed1992

DNA-coated carbon adsorbents experimental assessment and results of severe psoriasis treatment.

Newly developed combined adsorbents, containing from 1 to 8 mg of thymic DNA per 1 g of the granulated or the fibrous carbonic matrix, demonstrated good biocompatibility and selectivity for DNA- and DNP-binding substances. In a group of 14 patients with severe psoriasis (uncontrol trial) a single hemoperfusion procedure through DNA-coated granulated synthetic carbons (perfusion volume was 2.5-3.1 L, sorbent quantity was 30 g) resulted in complete remission in 6 patients and in substantial improvement of clinical status in 6 other patients. A positive effect was observed in 4 patients during 7-11 months; in 8 patients it is being observed for more than 29-33 months. The double-blind tests in the group of patients subjected to hemoperfusion through the DNA-coated charcoal (27 people) and uncoated charcoal (9 people) showed the full-scale remission in 55.5% and 11.2% respectively. The authors believe that the DNA-coated activated carbons can be an effective therapeutic procedure for treatment of numerous immuno-dependent diseases and states associated with disorders in the kinetics of cell division.

Antibodies, Antinuclear↗
PubMed1991

Immunoadsorption and plasma exchange in multiple sclerosis: complement and plasma protein behaviour.

Three groups of patients suffering from acute attacks or progressive multiple sclerosis (MS) are under investigation. First results revealed remarkable clinical improvements of patients with acute attacks in the groups treated by therapeutic plasma exchange (TPE) and immunoadsorption (IA). Only slight or no improvements were seen in the patients of the control group treated only with steroids. Plasma protein levels (IgG, IgM, IgA, fibrinogen) were considerably reduced in patients of the TPE group after each treatment procedure as expected. The same holds true concerning the total hemolytic capacities (THC) of the complement of the classic (CP) and the alternative (AP) pathway. On the other hand in the IA group only slight decreases of plasma proteins (about 20%) were observed, but the behaviour of THC's were quite similar than those seen in the patients of the TPE group. The THC decreases in both groups can be explained by removal of all complement factors (TPE group) or by the adsorption of single factors (IA group) of both complement pathways according to earlier in vitro investigations. The THC decreases in patients of both groups suffering from acute MS attacks could mean an "antiinflammatory" effect and could--at least partially--contribute to the clinical improvements of these patients.

Blood Proteins↗
PubMed1990

A newly developed LDL-binding material.

Results of in vitro and ex vivo experiments with a newly developed LDL-binding material are presented. This material consists of macroporous bead cellulose which is capable to bind selectively LDL. LDL-cholesterol is considerably decreased after contacts of plasma or serum samples with this bead cellulose. On the other hand high density lipoproteins (HDL) and other plasma components (proteins, enzymes, electrolytes, and metabolic substances) remained high or unchanged. Triglycerides (TG)--transported by very low density lipoproteins--are also bound up to a certain degree. 1 g of the adsorbent wet mass binds at least 20 mg cholesterol. The capacity suffices to decrease two- to threefold increased cholesterol and LDL plasma levels to the low normal range following passage of the sevenfold plasma volume' through two the three LDL adsorbent columns (results of perfusion experiments). In vitro and dog experiments revealed only slight drops of the hemolytic capacities of the classic complement pathway and moderate decreases of the alternative one. But no detectable side effects were noticed in the dog experiments.

Adsorption↗
PubMed1989

Low density lipoprotein binding by macroporous bead cellulose.

Cellulose of a certain macroporous structure is capable of binding selectively low density lipoproteins (LDL) whilst maintaining high density lipoprotein (HDL) levels. It was ascertained that the porous structure of the cellulose causes binding of LDL and that also diffusion processes play a role. To a certain extent special bindings such as hydrogen bonds between cellulose and LDL and/or hydrophobic interactions may furthermore be of importance for the LDL fixation.

Adsorption↗
PubMed1987

Adsorption of immunologically relevant molecules--its present and future.

The development of numerous adsorbents of various types oblige us to define the used terms. Adsorbents functioning on the same principle as that of the binding between antigen and antibody should be designated as specific adsorbents or immunoadsorbents. All other kinds of adsorbing materials act more or less selectively. A review is given concerning investigations about selective adsorbents. The removal of IgG antibodies is possible with adsorbents of the protein A type. Synthetic materials with IgE and IgM binding properties are presented and are under development. The blocking of the complement system by binding certain components and breakdown products can be a worthwhile feature in view of the biocompatibility of adsorbing materials.

Animals↗
PubMed1987

In vitro investigations with selective adsorbents for IgE and IgM.

In vitro studies were carried out with IM-T. This adsorbent consists of polyvinyl alcohol joined with tryptophan side chains and was delivered by ASAHI Medical Co., Ltd., Tokyo/Japan. It was found that IM-T binds immunoglobulins G and M and also immune complexes only moderately but IgE was adsorbed in remarkable amounts. A clear dose-dependent was adsorbed in remarkable amounts. A clear dose-dependent manner of the IgE adsorption could be stated. Kinetic studies revealed that the binding was only slow and gradual.

Animals↗
PubMed1984

Electrophoretic mobilities and levels of T and B lymphocytes in human pregnancy.

Automatic cell electrophoresis has been used to investigate electrokinetic properties and the levels of T and B lymphocytes in peripheral blood of 124 pregnant women at different stages of gestation, and in 44 healthy controls. The electrophoretic mobilities (EPM) of T and B lymphocytes in maternal peripheral blood vary only slightly throughout pregnancy. In the pregnant subjects, there is a small but significant reduction in the percentage of circulating T lymphocytes. It is concluded that an increased electrostatic repulsion between maternal lymphocytes and trophoblast cells by alterations in the surface charge of lymphocytes plays an unimportant role in protecting the allogenic fetus from maternal immune attack.

B-Lymphocytes↗
PubMed1983

[Immunoadsorption using Staphylococcus aureus, COWAN I strain].

Immunoadsorbents bind immunoglobulins, immunocomplexes or immunocytes. Their clinical use could become a new therapeutic principle for diseases in the pathogenesis of which antibodies or immunocomplexes play an important part. Immunoadsorbents are also being tested in the hope that they can at least partially replace the plasmapheresis-plasma exchange therapy at present practised. A report is given on in-vitro studies using protein-A-bearing staphylococci, strain COWAN I. The bacteria bind IgG selectively, IgM to a small extent, IgA, IgD and IgE hardly. The majority of the other plasma proteins tested (e.g. albumin, transferrin, ceruloplasmin, alpha-2-macroglobulin, beta-lipoprotein, alpha-2-glycoprotein, alpha-1-antitrypsin) are bound only non-specifically and in small quantities. The staphylococci can react with acid solutions (glycin-HCl buffer, acetic acid) in several ways. The activation of the complement system by IgG binding to protein A is one of the problems to be solved before immunoadsorbents an be used clinically.

Antigen-Antibody Complex↗
PubMed1980

[Circulating immune complexes in kidney diseases: pathogenic significance, methods of proof, problems, tendencies].

The tendency to recognize immune complexes already before their deposition in the tissue led in the seventies to the development of numerous methods of estimation for immune complexes in the serum and to the proof of these complexes in many diseases. The author enters these methods and their problems, the results got up to now for renal diseases, i. e. above all for glomerulonephritides, are cited in form of theses. Among others belong to this the establishments that the proof of circulating immune complexes cannot contribute to the diagnosis, but to the control of the activity of the diseases and of the therapeutic effect. Recently, research concerning immune complexes yielded remarkable results as to their role as regulating factors of the unspecific as well as of the specific defense mechanisms of the organism. This promises that further clarifications on etiology and genesis particularly of diseases of the immune complex type are to be expected, among them also for glomerulonephritides.

Antigen-Antibody Complex↗