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Biomedical subjects

E Berger

Publications and source records attributed to E Berger.

At least 19 recordsLinked to original sources
PubMed1979

A precursor of cytoplasmic actin in cultured Drosophila cells.

In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.

Actins↗
PubMed1979

Ten years of national health service in Canada.

Ten years ago the medical profession in Canada was conscripted into a compulsory national health insurance scheme without any prior consultation. Predictions that an open-ended, so-called "free" medical insurance scheme would lead to cost overruns and deterioration of medical services as well as inflationary trends have come true. Federal and provincial governments now attempt to contain costs by closing active treatment beds or filling them up with chronic patients and by attrition of existing facilities. Compulsory national health insurance has not led to lower morbidity nor greater longevity. It has only provoked an insatiable demand from the public for more "free" services, with the result that the system has become a quagmire of cost overruns and unfulfilled and unrealizable promises.

Canada↗
PubMed1979

Field dependence and short-term memory.

This study examined the relationship between field dependence and short-term memory. It was predicted that relatively field-independent individuals would perform better on short-term memory tests in which a large amount of interference is assumed to be present than would individuals who are more field-dependent. Subjects' appraisals of their own attentional facility, as well as their thoughts and feelings about the experimental tasks, were also elicited, and were looked at in terms of their relationship to field dependence and short-term memory.

Adolescent↗
PubMed1979

[Plexus paresis and smallpox vaccination (author's transl)].

Paresis of the left side upper plexus brachialis is diagnosed to a boy of 2;8 years. The trouble appeared after a smallpox vaccination, which had been carried out successfully. There are no symptoms of CNS-disturbance. serumneuritis and virogenic radiculatis are discussed as pathogenic variants. Although postvaccinal peripheral nervous lesions very seldom occur, it does seem possible that the ailment in question could be a consequence of the smallpox vaccination.

Brachial Plexus↗
PubMed1978

Frequency of minor nervous dysfunction in school children.

A study was made on 88 third grade school children to determine the frequency of minor nervous dysfunction (MND) Touwen and Prechtl). The study consisted of a neurological and an motoscopic examination as well as a visuomotor performance test (Gleiss). The following results were obtained: 1. Suspected MND was found in 15.9% of the population. 2. Only two children had optimal performance according to Prechtl's optimality concept. 3. If the diagnosis MBD is made excluding the behavioral description as a diagnostic criterion, no significant statistical correlation was found with school marks or social class. 4. The specific character of Lempp's "frühkindliches exogenes Psychosyndrom" and the behavioral disturbances described seems to us to be questionable. We found such disturbances with our methods as frequently in children with disturbed organic brain function as in children from a low social class.

Attention Deficit Disorder with Hyperactivity↗
PubMed1978

Adaptation of Drosophila enzymes to temperature--I. Acetylcholinesterase and NADP-dependent isocitrate-dehydrogenase.

1. The relationship between Km and assay temperature was examined in three tropical and two temperate Drosophila species, and in the cosmopolitan species, D. melanogaster, for isocitrate dehydrogenase and acetylcholinesterase. 2. For both enzymes Km patterns were similar among species from the same habitat, and different between habitats. No such parallelism was seen with respect to thermal inactivation. 3. The Q10 values in general reflected temperature dependent changes in Km, but exceptions were noted.

Acetylcholinesterase↗
PubMed1978

Erythrocyte calcium abnormalities and the clinical severity of sickling disorders.

We studied erythrocyte calcium levels and uptake in a group of patients with sickle haemoglobinopathies of different clinical severity in an attempt to relate these measurements to the production of irreversibly sickled cells and disease severity. Erythrocyte calcium levels were measured by atomic absorption spectroscopy and calcium uptake by isotopic means. In sickle cell anaemia, erythrocyte calcium content was elevated and the uptake of isotopic calcium increased under both oxygenated and deoxygenated conditions. There was a direct correlation between the numbers of irreversibly sickled cells and calcium uptake and an inverse relationship between calcium uptake and red cell potassium level. The clinical course of disease was milder in patients with high fetal haemoglobin levels, but there was no relationship between clinical course and calcium levels, calcium flux or irreversibly sickled cells. Our observations suggest that calcium accumulation and irreversibly sickled cell formation are related processes. The absence of good correlation between various biochemical and clinical parameters emphasizes the complexity of factors which modify the clinical course of this disorder.

Adolescent↗
PubMed1978

Calcium-induced damage of haemoglobin SS and normal erythrocytes.

An ionophore specific for divalent cations has been used to load normal erythrocytes and erythrocytes from patients with sickle cell anaemia (Hb SS disease), with small amounts of calcium. Such calcium accumulation leads to decreased cellular water, potassium, adenosine triphosphate (ATP), and osmotic fragility, all characteristics of irreversibly sickled cells (ISCs). In addition, calcium loading of Hb SS, but not normal, erythrocytes causes a marked decrease of haemoglobin oxygen affinity; another, and specific, hallmark of ISCs. Ionophore-induced accumulation of calcium by deoxygenated Hb SS erythrocytes also leads to temporary retention of sickled shape following reoxygenation, despite the absence of detectable intracellular haemoglobin S fibres. All these effects require calcium in the incubation medium and support the idea that increased intracellular calcium is important in the formation of ISCs in patients with Hb SS disease.

Anemia, Sickle Cell↗
PubMed1977

The ribosomes of Drosophila. Normal and defective ribosome biosynthesis in Drosophila cell cultures.

The assembly of proteins and RNA into mature ribosomal subunits has been studied in Drosophila cell cultures by pulse-chase experiments. Pulse labeled rRNA has a transit time of 3 h, while the transfer of ribosomal protein occurs completely within 30 min. Inhibition of protein synthesis by cycloheximide results in an almost immediate cessation of ribosome assembly, a result which indicates that no large pool of free ribosomal proteins exists in the cell. Substituting pre-ribosomal RNA with the analogue 5-fluorouridine (5-FU) results in a cessation of ribosome muturation. Under these conditions at least three large subunit proteins continue to accumulate on pre-existing cytoplasmic subunits, indicating an exchange. A portion of ribosomal subunit proteins synthesized in the presence of 5-FU can be recovered in cytoplasmic subunits once the effect of 5-FU has been reversed. This is most easily interpreted in terms of their stabilization on substituted pre-rRNA within the nucleolus, and subsequent utilization on unsubstituted RNA.

Adenosine↗
PubMed1977

Isozyme and allozyme patterns in embryonic Drosophila cell culture lines.

Two independently derived embryonic Drosophila cell culture lines were examined for 19 gene-enzyme systems. At two loci, alpha-glycerophosphate dehydrogenase on chromosome 2, and isocitrate dehydrogenase on chromosome 3, allelic variation was detected. These can now serve as genetic markers to identify hybrid cell clones. Quantitative differences between cell lines were found for five enzymes.

Alleles↗
PubMed1977

Host heme catabolism in drug-sensitive and drug-resistant malaria.

Chloroquine resistance has arisen in both human and murine forms of malaria. CR Plasmodium berghei in mice does not produce the malaria pigment which is characteristic of the CS form. Determinations of carbon monoxide production (i.e., host heme catabolism) by individual mice revealed that those infected with CS P. berghei produce only one fourth as much carbon monoxide as do CR infected mice at all levels of infection. These observations confirm the idea that malaria pigment is composed of precipitated host cell hemoglobin and suggest that drug resistance is accompanied by a basic alteration in parasite-mediated hemoglobin catabolism.

Animals↗
PubMed1977

[Teamwork in juvenile social work as an instrument in crisis intervention].

The changing emphasis in psychiatric care demands the use of new strategies and the development of sociopsychiatric institutions. The term 'crisis intervention' is suitable in this respect insofar as it also includes the aspects of prevention and follow-up care. An attempt at the development of a model for multiprofessional teamwork in juvenile social work is outlined under the aspect of sectorised sociopsychiatric care. The exhaustion of the basically available possibilities is at the moment limited due to structural difficulties.

Adolescent↗