Biomedical subjects
Publications and source records attributed to E Berger.
Two independently derived embryonic Drosophila cell culture lines were examined for 19 gene-enzyme systems. At two loci, alpha-glycerophosphate dehydrogenase on chromosome 2, and isocitrate dehydrogenase on chromosome 3, allelic variation was detected. These can now serve as genetic markers to identify hybrid cell clones. Quantitative differences between cell lines were found for five enzymes.
Chloroquine resistance has arisen in both human and murine forms of malaria. CR Plasmodium berghei in mice does not produce the malaria pigment which is characteristic of the CS form. Determinations of carbon monoxide production (i.e., host heme catabolism) by individual mice revealed that those infected with CS P. berghei produce only one fourth as much carbon monoxide as do CR infected mice at all levels of infection. These observations confirm the idea that malaria pigment is composed of precipitated host cell hemoglobin and suggest that drug resistance is accompanied by a basic alteration in parasite-mediated hemoglobin catabolism.
Explore the source record for details and available documents.
The changing emphasis in psychiatric care demands the use of new strategies and the development of sociopsychiatric institutions. The term 'crisis intervention' is suitable in this respect insofar as it also includes the aspects of prevention and follow-up care. An attempt at the development of a model for multiprofessional teamwork in juvenile social work is outlined under the aspect of sectorised sociopsychiatric care. The exhaustion of the basically available possibilities is at the moment limited due to structural difficulties.
Explore the source record for details and available documents.
The base sequence complexity of Drosophila transfer RNA (tRNA), 5S RNA, and 18S + 28S ribosomal RNA was determined by analyzing the kinetics of RNA-DNA hybridization on membrane filters. We find that Drosophila tRNA is made up from about 59 basic nucleotide sequences distinguishable by hybridization, suggesting that many of the 99 tRNA species resolved by reverse phase chromatography (RPC-5) are homogenic. In contrast 5S RNA was found to contain a single family of sequences. Either 18S ribosomal RNA (rRNA) alone, or 18S + 28S rRNA together, behaved kinetically as two sequence families, and the possible basis for this unexpected result is discussed.
One- and two-dimensional gel electrophoresis was employed to characterize and compare ribosomal subunit proteins from wild-type D. melanogaster and several mutants, including suppressor-of-forked and four X-linked cold sensitive lethals. The sibling species D. simulans was also studied. We found the electropherogram patterns indistinguishable in all cases.
The extent of interspecific homology between D. melanogaster and D. virilis for ribosomal RNA and ribosomal protein was examined using the techniques of two-dimensional gel electrophoresis, and RNA-DNA filter hybridization. Only 2 of the 71 ribosomal proteins resolved were found to be species specific, while comparisons of soluble larval hemolymph protein patterns showed little similarity. Depending on the technique employed, the sequence homology for 18S + 28S ribosomal RNA was found to be between 83-94%, and sequence homology for 5S rRNA was judged to be complete.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The general principles in the treatment of the disordered, is the change from segregation to social integration. The allocation of financial resources is necessary for the needed conditions, that means, that instead of the traditional institutions but mostly the ambulatory institutions, an early apprehension therapy should be financed. The seriousness in the treatment next to the specific facilities for the disordered, is the individual psychic help needed in regard to the secondary psychic problems, as a result of his disorder, also the socialization of the disordered should be advanced. Social organizations for disordered, as well as the parents of disordered children have a very important task, but the organizations can only fullfill the work in cooperation with a municiple care system.
A biochemical comparison was made between alpha-glycerophosphate dehydrogenase allozymes from Drosophila melanogaster. Enzymes extracted from the three major genotypes were indistinguishable in terms of their pH optima and thermal stabilities. Distinctive differences were observed for three parameters; temperature dependence of specific activity, temperature dependence of K-m, and reaction rate constancy over a physiological temperature range. These results are discussed in terms of a model of balancing selection and the existence of spatial and temporal allele frequency clines in natural populations.
Explore the source record for details and available documents.
Arteriovenous fistulas constructed from preserved (frozen) allograft veins provided vascular access for maintenance hemodialysis in 14 patients with inadequate vasculature for conventional autogenous fistulas. Electromagnetic flows ranged from 200 to 250 cc/min. Dialysis flow of 200 cc/min were accomplished consistently. The allografts were useable for dialysis much earlier than autogenous A-V fistulas. Arteriography demonstrated a wide vasculature channel very suitable for dialysis needles. Allografts seemed superior to bovine heterografts in both patency and susceptibility to infection. Scanning electron microscopy revealed smoother initimal surfaces if the donor received small amounts of heparin systemically while the veins were being removed. Allograft veins appear to be a useful alternative means of vascular access for hemodialysis.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.