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E DeHarven

Publications and source records attributed to E DeHarven.

5 recordsLinked to original sources
PubMed1991

Translocation (14;19) in acute biphenotypic leukemia.

Translocation (14;19)(q32;q13.1) is an acquired chromosomal rearrangement that has been associated with chronic lymphocytic leukemia of B-cell phenotype frequently progressing to lymphoma. Molecular analysis suggests that the translocation involves the immunoglobulin heavy chain gene on chromosome 14 and the BCL3 oncogene on chromosome 19. We present the first case of t(14;19) in a patient with acute leukemia. Correlation of detailed cytogenetic and molecular genetic studies, cell surface marker analysis, cytochemistry, and electron microscopy indicated that the leukemic cells were biophenotypic, with characteristics consistent with both myeloid and B-lineage lymphoid differentiation.

Adult↗
PubMed1985

Acute inclusion-body leukemia. Case report and review of literature.

An unusual case of acute leukemia with basophilic cytoplasmic inclusion bodies in a child is described. Certain aspects of the case suggested a lymphoblastic phenotype and others a myeloid origin. The inclusions stained positively for acid phosphatase and toluidine blue. Too few patients have been reported to determine the prognostic significance of acute leukemia with inclusion bodies.

Basophils↗
PubMed1972

Theta particles: a structure found in hamster sarcoma virus.

Hamster sarcoma virus (HaSV), a ribonucleic acid tumor virus, pelleted from tissue culture fluid manifests type C morphology by electron microscopy. However, if virus is first concentrated by polyethylene glycol or ammonium sulfate followed by density gradient banding, the virus shows a dramatically atypical barred core structure, termed "theta particles." This structure suggests a condensation of the ribonucleoprotein into a flat disc. Atypical particles are found with HaSV and not in similarly treated feline leukemia virus or Rauscher-murine leukemia virus. Differences in the composition of HaSV as compared with these other viruses may be responsible for the production of such particles.

Ammonium Sulfate↗
PubMedDate not supplied

Distribution of a novel nuclear protein in normal and regenerating liver cells.

Monoclonal antibodies to a rat liver nuclear protein (B2, 68 kda, pI: 6.5-8.2) have been established and characterized to localize the distribution of this antigen in nuclear organization. It was demonstrated that this nuclear protein B2 is associated with actively transcribed nucleosomes and the nuclear matrix as revealed by immunogold labelling. In the regenerating liver cell, the immunogold particles are predominantly in the euchromatin as compared to the resting liver cells which are mainly in nuclear matrix and heterochromatin. It was also demonstrated that the incorporation of 32P into the nuclear protein B2 is increased 6-fold in the actively transcribed nucleosomes as compared to the nuclear matrix, as analyzed by two-dimensional polyacrylamide gel electrophoresis. We hypothesize that this nuclear protein may act as an anchorage point either for actively transcribed RNA or for DNA replication. Once this protein is highly phosphorylated, it dissociates from the nuclear envelope and can then dynamically interact with active nucleosomes within the nucleus.

Animals↗