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Biomedical subjects

E Eylan

Publications and source records attributed to E Eylan.

At least 73 records · Page 4Linked to original sources
PubMed1975

Experimental and clinical studies of 5-fluorocytosine activity in candida ocular infections, II. The effect of 5-fluorocytosine in treating experimental candida infection in rabbits' eyes.

Mild and severe experimental ocular infections in rabbits' eyes were induced by inoculation of Candida albicans with or without addition of dexamycin drops. These rabbits were treated with 5-Fluorocytosine, topically, orally and in a combined topical and oral manner. The topical treatment was effective only in mild infections. Severe infections responded to oral and especially to combined oral-tropical treatment.

Administration, Oral↗
PubMed1975

Mast cell sensitizing antibodies in experimental candidiasis.

Inoculation into foot-pads of guinea-pigs of a living suspension of Candida albicans together with Complete Freund's Adjuvant, provoked a hypersensitive state, characterized by: 1) positive skin tests; 2) direct degranulation of peritoneal mast cells when exposed in vitro to the antigen; and 3) homologous "mast cell sensitizing antibodies" detected by the "indirect mast cell degranulation test". The mast cell sensitizing antibodies were heat labile, and their activity could not be restored by complement.

Animals↗
PubMed1975

Mast cell sensitizing antibody (MCSAb) response in experimental candidiasis: chromatographic studies.

A single dose of the yeast-like fungus Candida albicans (CA), mixed with Complete Freund's Adjuvant (CFA), and inoculated into guinea-pig foot-pads, provoked the productions of homologous mast cell sensitizing antibodies (MCSAb) present in the psiI-globulin fraction only, and of agglutinins localized in the same fraction. Heterologous MCSAb and skin-sensitizing antibodies detectable by passive cutaneous anaphylaxis (PCA) were constantly absent. These facts are in contradiction to results of previous experiments performed with other antigens; which after similar inoculation elicited production of heterologous MCSAb attaching themselves on rat mast cells (MC) and present in the psi2 fast moving globulin, homologous MCSAb localized in the psiI-globulin and in the psi2 fast moving globulin, and PCA antibodies. The immunogenicity of CA is discussed.

Animals↗
PubMed1975

Antigenic relationships between Candida albicans and various yeasts as reflected by immunoglobulin-class specificity.

A group of guinea pigs was inoculated into the foot pads with a single dose of Candida albicans in complete Freund's adjuvant, while another group was similarly inoculated once in the foot pads but also several times intramuscularly, with Candida alone. All guinea pigs were bled at different intervals after immunization and sera were separated chromatographically into IgG and IgM fractions. In order to study the antigenic relationships as reflected by immunoglobulin-class specificity, IgG and IgM fractions and whole sera obtained from guinea pigs differently immunized, were tested for the presence of agglutinins against C. albicans, six other species of Candida, and species of the ascosporogenous genera Saccharomyces, Kluyveromyces and Schizosaccharomyces. The results show that (1) only IgG fractions of the different sera prepared contained the specific anti-C. albicans antibodies; (2) IgG and IgM fractions of the sera obtained from a single inoculation did not reveal a specific pattern expressing antigenic relationships of the yeast studied, and (3) the IgM fractions of the sera obtained from several inoculations had a more homogenous pattern of reactivity, since mainly these contained the agglutinins against the ascosporogenous yeast species.

Agglutination Tests↗
PubMed1975

Experimental and clinical studies of 5-fluorocytosine activity in Candida ocular infections. I. In vitro activity of 5-fluorocytosine on Candida species isolated from ocular infections.

In vitro sensitivity to 5-fluorocytosine (5-FC) of 35 Candida strains (14 C. albicans, 10 C. tropicalis, 5 C. parapsilosis, 2 C. guilliermondii, 2 C. krusei, 2 C. pseudotropicalis), isolated from the eyes of patients suffering from various external ocular inflammations who had not been treated with 5-FC, was tested and compared to that of standard Candida species. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of 5-FC for the strains isolated from ocular infections ranged from 0.06 to 1.95 mug/ml and from 0.24 to 7.81 mug/ml, respectively, indicating that no primary resistant strains to 5-FC were found in this series.

Animals↗
PubMed1975

Yeasts in banal external ocular inflammations.

In 53 out of 313 patients suffering from longstanding inflammations of the outer eye or dacryocanaliculitis, not yielding to the obligate treatment with antibiotics and steroids, yeasts were isolated and regarded as the causative agents. Among the yeasts, different Candida species were prevalent, with C. albicans and C. guillermondii at the top. An obstinate, superificial epithelial and subepithelial punctate keratitis appeared to represent an entity pathognomonic of a yeast infection. Eradication of the yeasts and cure of the inflammation was achieved only by intensive prolonged treatment with Mycostatin or amphotericin B.

Acute Disease↗
PubMed1975

Confirmation of the protective role of proteins from S. typhimurium in infection of mice with their natural pathogen.

The protective role of proteins from S. typhi-murium in infection of mice with their natural pathogens was confirmed. Hyperimmunization with either toxic or detoxified proteins, from the same batch, induced the synthesis of strongly precipitating antiprotein antibodies, and the mice were protected against infections with 15-75 LD100 of S. typhimurium. Reinfection of the same animals with 300-500 LD100 of S. typhi-murium induced, in the vaccinated mice, the synthesis of only anti-protein antibodies precipitating in agar-gel.

Animals↗
PubMed1975

Nucleic acid homology studies among Candida albicans, Syringospora albicans and Leucosporidium species.

Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization technique was used to investigate nucleic acid homologies among acid Candida albicans, Syringospora albicans and the Leucosporidium species (L. nivalis, L. capsuligenum, L. frigidum, L. gelidum, L. antarcticum). The nucleic acid homology between C. albicans, S. albicans and the Leucosporidium species was very low (ranging from about 7-11% for C. albicans and 8-12-5% for S. albicans), indicating the unrelatedness of C. albicans to the basidiomycetous yeast genus Leucosporidium.

Basidiomycota↗
PubMed1975

A spectrophotometric method for quantitative determination of lysozyme in human tears: description and evaluation of the method and screening of 60 healthy subjects.

A spectrophotometric micromethod for lysozyme determination in tears is described, which enables the estimation of very low concentrations of lysozyme in individual tear samples, and which can be performed rapidly. The method is based on collecting the tears by a microcapillary tube and diluting them in a special manner which increases the volume of the tear sample, thus making possible analysis of other tear constituents in addition to lysozyme. Lysozyme activity in tears is determined by reduction of optical density (OD) of Micrococcus lysodeikticus suspension. The sensitivity of the method was determined on the basis of repetitive readings. The level of lysozyme in tears of 60 healthy people was determined by this method and found to be 6.1 mg. per milliliter hen egg lysozyme (HEL) with standard deviation of 1.57 mg. per milliliter HEL or 1.5 mg. per milliliter human tear lysozyme (HTL) with standard deviation of 0.39 HTL. Lysozyme level in tears of both eyes of each individual is equal, and any difference observed between the two eyes is due to the variability of the method.

Adolescent↗
PubMed1975

Antigenic, antibiotic and phage receptor activity of proteins from Sh. boydii 15.

The analyzed strain of Sh. boydii 15 induced antibodies against the somatic antigen conjugate, as in most Shigella. Except for the proteinic carrier of the somatic antigen, only a few of the homologous proteinic determinants induced corresponding antibodies; these were illustrated with the help of a serum to proteins from Sh. sonnei. The proteins of Sh. boydii 15 displayed a wide range of antibiotic activity against Shigella, E. coli and Salmonella. Another aspect concerning the biological activities of the proteins isolated from Sh. boydii 15 was its temperate phage receptor activity. This activity was found as well in the free proteins as in the proteinic moiety of the somatic antigen.

Animals↗
PubMed1975

Heterologous protections in experimental salmonellosis.

Immunizations of mice with proteins from S. paratyphi B protected the animals against infection with a concentration of S. paratyphi C which killed the controls and against an infection with 50 LD100 of the homologous S. paratyphi B. The sera of the infected mice showed common precipitation lines of proteins from the two species belonging to different serogroups. Consecutive inoculations with S. typhimurium of both groups of vaccinated mice protected the animals against the infection with their natural pathogen. Immunizations with proteins from S. cholerae-suis protected about 70% of the mice infected with S. paratyphi B and with S. paratyphi C; a higher protection was not, however, induced against infection with the homologous strain. Consecutive infections with S. typhi-murium of the mice resulted in total protection of the animals previously inoculated with S. paratyphi B and S. paratyphi C; the group of mice infected with S. cholerae-suis was less protected against the subsequent inoculation with S. typhi-murium (about 50%). In the infections of mice, S. paratyphi B and S. paratyphi C, seem related to S. typhi-murium by common proteins; the proportion of common protective determinants is not yet known.

Animals↗
PubMed1975

Unrelatedness between agglutinations of bacteria and the presence of anti-polysaccharides in sera of Salmonellae from groups B and D.

Rabbits hyperimmunized with two strains of S. paratyphi B provided sera with high agglutinating titers, although the sera were deprived of antipolysaccharide antibodies. However, the polysaccharides of both strains of S. paratyphi B precipitated against anti S. typhimurium sera in which antipolysaccharide antibodies were induced. Antisera obtained with three S. enteritidis strains showed a comparable unrelatedness between the agglutinating titers and the presence of antipolysaccharide antibodies in the corresponding sera. One of the strains, repeatedly used for immunization, never induced antipolysaccharide antibodies, while the agglutinating titers of the sera were high; the polysaccharide of this strain precipitated strongly against anti-Salmonella typhi O 901 sera and against the antisera of the other S. enteritidis strains in which antipolysaccharide antibodies were induced. According to these results, there is no apparent relationship between agglutinations of bacteria and the presence of antipolysaccharide antibodies in the corresponding sera. Heated proteins, isolated from all the strains after previous exhaustive extraction at 37 degrees C, showed strong serological precipitations.

Agglutination Tests↗