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Biomedical subjects

E Friedrich

Publications and source records attributed to E Friedrich.

At least 55 records · Page 3Linked to original sources
PubMed1981

Lectin mediates homing of sialidase-treated erythrocytes of the liver as revealed by scintigraphy.

Mammalian erythrocytes loose their normal circulatory pattern following desialylation by sialidase and are trapped in the liver. The mechanism responsible for this phenomenon has been studied by a new scintigraphic method. We report here that the retention of asialo-erythrocytes in the liver is due to the interaction between a lectin-like receptor on Kupffer cells and terminal D-galactosyl residues exposed on erythrocytes after sialidase treatment. The major findings supporting the conclusion are: First, kinetics of asialo-erythrocyte accumulation in the liver are identical in conventional and germfree animals, demonstrating that the presence of serum antibody is not essential. Second, trapping of asialo-erythrocytes can be substantially inhibited by intravenous injection of N-acetyl-D-galactosamine or galactosylated bovine serum albumin, other saccharides or glycoproteins are less or not at all effective. This specificity pattern is characteristic for the D-galactose-specific lectin on Kupffer cells. It therefore appears that the retention of sialidase-treated erythrocytes in the liver is lectin- and not antibody mediated.

Animals↗
PubMed1981

[Diagnostic possibilities of the function of the fetal thyroid gland by amniocentesis (author's transl)].

The assessment of fetal thyroid function by measurement of reverse triiodothyronine (RT3) in amniotic fluid (AF) seems to be a valuable diagnostic tool in detecting fetal hypothyroidism. The concentrations of RT3 were measured by radioimmunoassay in 346 samples of AF which were obtained by transabdominal amniocentesis. Among these, 323 were obtained from normal pregnancies between weeks 8 and 41 of gestation and the remaining 23 from various complications of pregnancy: Rh-isoimmune disease, Down's syndrome, Klinefelter's syndrome (XXY), M. Krabbe, and anencephaly. The highest concentrations of RT3 in AF were observed between weeks 15 to 20 of gestation, followed by a gradual decrease with advancing gestational age. In complicated pregnancies a similar distribution of RT3 values was found. It is suggested that RT3 in AF should be measured in order to assess fetal thyroid function whenever amniocentesis is performed between weeks 16 and 20 of gestation, in particular when hypothyroidism of the fetus is suspected.

Amniocentesis↗
PubMed1981

Galactosyl specific receptor on liver cells: binding site for tumor cells.

In this paper the recognition of various rat tumor cells by rat liver cells is demonstrated in vitro. A liver cell receptor involved in the binding process has been identified. The ultrastructure of the cell contacts was examined by transmission electron microscopy. Hepatocytes and Kupffer cells were isolated from rat liver by collagenase treatment and cell adhesion tests were performed with 4 different tumor cells types. Hepatocytes were found to bind Walker sarcoma cells, lymphoma cells and Yoshida hepatoma cells but not leukemia 5222 cells. Kupffer cells bound all tumor cell types. Normal blood cells were not bound under the same conditions. Recognition of tumor cells by hepatocytes was mediated by a galactose specific lectin on the liver cell surface as shown by hapten inhibition experiments with specific saccharides. Although Kupffer cells express a similar lectin-like receptor adhesion of tumor cells could not or only slightly be inhibited by galactose or related saccharides. It is concluded that the spontaneous adhesion of tumor cells to liver cells in vitro is a specific recognition event which in part is mediated by lectin-carbohydrate interactions.

Animals↗
PubMed1980

[The quantitative immunochemical determination of specific antibodies against Candida albicans within the scope of immunological testing in the malignant melanoma (author's transl)].

Precipitating antibodies against a polysaccharide antigen from Candida albicans were in the human serum determined quantitatively by the aid of a reversed radial immunodiffusion technique. The gel, therewith, contained the antigens, whilst into the gel's punching holes the serum specimens and the calibrated standards were filled in. Amplifying examinations of the course in patients suffering from malignant melanoma, the findings of the methods used hitherto were compared within the scope of an immunological status (in vivo-skin testing by dinitrochlorobenzene, Candida antigen, trichophytin, and tuberculin, precipitation test after Ouchterlony, immunofluorescence-optical evidence of Candida antibodies as well as determination of the Candida antibodies. In contrast to the hitherto available qualitative or semi-quantitative methods evidencing humoral antibodies, we consider the feasibility of simply workable quantitative determinations of antibodies to be an enrichment of the scale of methods. The reversed Mancini technique, simultaneously, is appropriate to determine specific antibodies against other diagnostically relevant antigens, so far as precipitating antigen/antibody complexes are formed. Therewith, commercially available testing kits could favour the standardization.

Antibodies, Fungal↗
PubMed1979

[The quantitative immunochemical determination of Candida albicans-specific antibodies (author's transl)].

A diffusion method is described for quantitative determination of precipitating antibodies against polysaccharide antigen from Candida albicans. It is a reversed single radial immunodiffusion method with the antigen in the gel layer. As one parameter within an immunological testing programme antibody levels were estimated in serum samples from a patient suffering from malignant melanoma.

Antibodies, Fungal↗
PubMed1979

[Comments and the diagnosis of vitality of early pregnancy in threatening abortion].

In a series of 79 consecutive patients examined by sonar and assays of human chorionic gonadotrophin in the first half of pregnancy 41 ultimately aborted. On analysis of the sonar and post abortion findings it was found, that the aborted pregnancies fell into 3 clearly defined groups: blighted ova, missed abortions and hydatidiform moles. Strict diagnostic sonar criteria of abnormality were established for these groups. Assays of human chorionic gonadotrophin have shown to be useful guides. But the overlap between their normal and abnormal ranges does not permit their use as sole arbitors of which pregnancies should be terminated.

Abortion, Missed↗