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Biomedical subjects

E Heath

Publications and source records attributed to E Heath.

At least 37 records · Page 2Linked to original sources
PubMed1981

Specific cleavage of bovine parathyroid hormone catalyzed by an endopeptidase from bovine kidney.

Cleavage of parathyroid hormone (PTH) is catalyzed by an endopeptidase associated with a partially purified membrane preparation from bovine kidney cortex. This enzyme was found to have an acid pH optimum and to be easily extracted from the membranes by a single freeze-thaw cycle. The cleavage is remarkable in that it appears to be restricted to a small region of the PTH peptide chain, generating fragments which are not further degraded. The dominant products are a large fragment, COOH-terminal in origin, and a small fragment from the NH2 terminus. The small fragment is biologically active and its activity establishes that it contains at least the first 29 amino acids in PTH. The large fragment has no biological activity. The cleavage of PTH was demonstrated both with iodinated PTH and with unlabeled hormone by immunoassay and by labeling the large fragment after its production. Microsequencing of the large fragment showed that, in fact, two products are produced: one with its NH2 terminus at position 38 of PTH and one with its NH2 terminus at position 35. These fragments are remarkably similar to those generated in both the liver and the kidney in vivo.

Animals↗
PubMed1981

Plasma cortisol in Bos taurus and Bos indicus heifers in seasonal tropical climate.

Cortisol concentration in blood plasma was measured in Brown Swiss, Holstein-Friesian, and White Fulani heifers over two consecutive estrous cycles during each of the four quarters of the hot humid seasonal tropical climate of Ibadan, Southern Nigeria. Basal cortisol in plasma was low (between 1 and 10 ng/ml). Differences between breeds were not significant. Averages and increases with proestrus and estrus were higher in the wet season (March to August) than in the dry season (November to January). Packed cells volume was higher in the dry season than in the wet season. Seasonal changes in rhythmicity of the estrous cycle were not significant. The low cortisol in plasma probably reflects adaptation to the hot climate and a mechanism to prevent high metabolic heat.

Adrenal Cortex↗
PubMed1980

Blockade by l-lysine of non-narcotic analgesics.

The antinociceptive effects of the non-narcotic analgesics clonixin, flunixin, acetylsalicylic acid, aminopyrine and phenylbutazone in the yeast paw test were blocked by l-lysine. Blockade occurred at doses of l-lysine which did not affect pain threshold. The site(s) or mechanism of action of blockade could not be determined with certainty. It appears unlikely that l-lysine prevented the analgesics from getting to active sites since plasma or brain levels of flunixin were not altered for up to 2 hr after drug administration and binding of flunixin to plasma protein was not affected. Blockade by l-lysine appears to occur at least in part at a peripheral site since it was not blockade by l-arginine or l-ornithine which compete for a common transport system with l-lysine and, hence, would have reduced the effect of l-lysine if its actions were central. However, blockade within the central nervous system cannot be ruled out. Antagonism by l-lysine does not seem to involve endogenous serotonin since it was not reversed by the serotonin precursor, 5-hydroxytryptophan, or by fluoxetine, a specific blocker of serotonin uptake. In contrast to the block of non-narcotic analgesics, l-lysine potentiated the antinociceptive effects of morphine.

Aminopyrine↗
PubMed1979

Persistent truncus arteriosus communis in a two-year-old steer.

A two-year-old Hereford-cross steer was presented for routine embalming. Dissection of the heart and great vessels revealed a persistent truncus arteriosus type A2. Despite complete mixing of pulmonary and aortic blood, this animal had survived to adulthood.

Animals↗
PubMed1979

Relationships of musculus rhomboideus, ligamentum nuchae and vertebrae thoracis and lumbales in Bos indicus.

The funicular part of the ligamentum nuchae of Bos indicus attaches to the dorsolateral edges of the bifid spines of the seventh thoracic through third lumbar vertebrate. Cranially, the funiculus nuchae runs freely medial to the deep thoracic fascia which separates it from the overlying rhomboideus thoracis. The zebu hump is composed primarily of rhomboideus cervicis which in the thoracic position is clearly separable from underlying structures.

Animals↗
PubMed1977

Ultrastructural effects of perfomic acid oxidation of epoxy-embedded tissue with and without early uranyl staining.

Epoxy-embedded blocks of glutaradehyde and OSO4 fixed adenohypophyseal tissue were immersed in performic acid, rinsed and sectioned for electron microscopy. Sections exhibited a general image intensity equivalent to control sections but their stainability with both uranyl acetate and lead citrate stains was increased. It was concluded that osmium was not removed by performic acid oxidation. Membranes of endoplasmic reticulum and the Golgi apparatus were not visible, and other components (acidophil granules, in particular) were markedly distored in shape after oxidation. Such changes, however, were not evident in specimens previously exposed to uranyl acetate during ethanol dehydration.

Animals↗
PubMed1977

Granulomas of the testis and excurrent ducts following vasoligation in the fowl (Gallus domesticus).

Ten adult 18-month-old male fowls were vasoligated unilaterally just anterior to the cloaca via an incision made between the base of the tail and the cloacal orifice. Two birds each were killed at two weeks and four weeks post-ligation, and the remaining six birds at 12 weeks post-ligation. Forty per cent of the birds showed severe reactions in the ipsilateral testis, epididymal region and ductus deferens. The remaining birds showed slight gross changes and near normal histology. The contralateral organs in all birds were normal both macroscopically and microscopically.

Animals↗
PubMed1975

Biomedical applications of vapor phase analysis.

Recent investigations of physiological systems have demonstrated that even minor chemical constituents can play a major role in mediating physiological responses. Recognizing this, the investigator seeks to isolate or selectively quantify the concentration of a specific drug or hormone that is available to target tissue. Today, as drugs are developed which are more and more potent, greater demands are made on analytical methodology for ever more sensitive yet specific techniques of quanification. The techniques of vapor phase analysis such as gas chromatography, especially in combination with mass spectrometry, offer great promise to investigative pharmacology/toxicology. Some of the potentials and limitations of vapor phase analysis will be described. More detailed descriptions of basic gas chromatography (McNair and Bonelli, 1969; Ettre and McFadden, 1969) and combined gas chromatography-mass spectrometry (McFadden, 1973) can be found elsewhere.

Blood Chemical Analysis↗